Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice

Grigera, Pablo R.; Marzocca, Marina Patricia; Capozzo, Alejandra Victoria; Buonocore, Linda; Donis, Ruben O.; Rose, John K.

doi:10.1016/s0168-1702(00)00164-7

Cited by 25 publications

(20 citation statements)

References 35 publications

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“…The ability of the VSV genome to tolerate foreign transcription units and genes and to accept the replacement of VSV G with a foreign transmembrane glycoprotein has been extremely helpful for studies on the structure and function of these foreign proteins in the context of virus infection (23,31). In addition, rVSVs are being investigated as promising live virus vaccine candidates for several viruses, such as influenza virus, human immunodeficiency virus, and bovine viral diarrhea virus (14,33,36,37). The potential to serve as vaccine candidates is related to the high-titer growth of VSV and rVSVs in many cell lines (20,23,25), the elucidation of a strong cellular and humoral immune response by VSV in vivo (11,24,53), and the normally asymptomatic or mild clinical course of VSV infections in humans (35,48).…”

Section: Resultsmentioning

confidence: 99%

Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses

Garbutt

Liebscher

Wahl‐Jensen

et al. 2004

J Virol

333

338

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Replication-competent recombinant vesicular stomatitis viruses (rVSVs) expressing the type I transmembrane glycoproteins and selected soluble glycoproteins of several viral hemorrhagic fever agents (Marburg virus, Ebola virus, and Lassa virus) were generated and characterized. All recombinant viruses exhibited rhabdovirus morphology and replicated cytolytically in tissue culture. Unlike the rVSVs with an additional transcription unit expressing the soluble glycoproteins, the viruses carrying the foreign transmembrane glycoproteins in replacement of the VSV glycoprotein were slightly attenuated in growth. Biosynthesis and processing of the foreign glycoproteins were authentic, and the cell tropism was defined by the transmembrane glycoprotein. None of the rVSVs displayed pathogenic potential in animals. The rVSV expressing the Zaire Ebola virus transmembrane glycoprotein mediated protection in mice against a lethal Zaire Ebola virus challenge. Our data suggest that the recombinant VSV can be used to study the role of the viral glycoproteins in virus replication, immune response, and pathogenesis.

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Section: Resultsmentioning

confidence: 99%

Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses

Garbutt

Liebscher

Wahl‐Jensen

et al. 2004

J Virol

333

338

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“…A number of recombinant negative-strand RNA viruses expressing foreign proteins have been constructed. Recombinant vesicular stomatitis viruses (VSVs) are able to express the human CD4 protein (32), the influenza virus hemagglutinin (HA) and neuraminidase (14,25,27), the respiratory syncytial virus G and F proteins (12), the human immunodeficiency virus Gag and Env proteins (10), and the bovine viral diarrhea virus E2 protein (9). Their efficacies as vaccine vectors have been studied (reviewed in reference 26).…”

mentioning

confidence: 99%

Recombinant Newcastle Disease Virus as a Vaccine Vector

Nakaya

Cros

Park

et al. 2001

J Virol

224

223

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A complete cDNA clone of the Newcastle disease virus (NDV) vaccine strain Hitchner B1 was constructed, and infectious recombinant virus expressing an influenza virus hemagglutinin was generated by reverse genetics. The rescued virus induces a strong humoral antibody response against influenza virus and provides complete protection against a lethal dose of influenza virus challenge in mice, demonstrating the potential of recombinant NDV as a vaccine vector

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“…The arginine residue is also observed in the amino-acid sequence of membrane anchors of our recombinant E2. However, surface localisation of E2 has also been observed in cells infected with recombinant baculovirus [39] or recombinant VSV [21] that express E2. As suggested by Köhl et al [34,35] it is conceivable that, in some transfected cells, the cell retention machinery becomes saturated by overexpressed E2 glycoproteins which are then transported to the cell surface.…”

Section: Discussionmentioning

confidence: 99%

“…Neutralising antibodies and a cellular response are produced in animals vaccinated with various recombinant viruses expressing E2 [3,17,21], with DNA plasmid constructs expressing E2 [26,42,45] or with E2 subunit vaccines [7,11,12,39,58]. Purified recombinant E2 from BVDV1a strain Singer lead to partial protection against acute infection by the homologous strain [7].…”

Section: Bovine Viral Diarrhoea Virus (Bvdv)mentioning

confidence: 99%

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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-We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.BVDV / DNA immunisation / E2 / neutralisation / synthetic gene

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Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice

Cited by 25 publications

References 35 publications

Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses

Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses

Recombinant Newcastle Disease Virus as a Vaccine Vector

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

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