Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Couvreur, Bernard; Letellier, Carine; Olivier, Fabrice; Dehan, Pierre; Elouahabi, Abdelatif; Vandenbranden, Michel; Ruysschaert, Jean Marie; Hamers, Claude; Pastoret, Paul-Pierre; Kerkhofs, Pierre

doi:10.1051/vetres:2007037

Cited by 7 publications

(2 citation statements)

References 60 publications

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“…The selection of E2 and NS3 proteins as antigen targets for a BVDV vaccine has been established previously. Our own studies and that of others tested the efficacy of E2-based vaccines and showed an encouraging level of partial protection from BVDV challenge [6,20,[24][25][26][27][28][29]. Vaccination of cattle with the DNA vaccine expressing the highly immunogenic E2 induces protective immunity [6].…”

Section: Discussionmentioning

confidence: 72%

Enhanced neutralising antibody response to bovine viral diarrhoea virus (BVDV) induced by DNA vaccination in calves

El-Attar

Thomas

Luke

et al. 2015

Vaccine

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Section: Discussionmentioning

confidence: 72%

Enhanced neutralising antibody response to bovine viral diarrhoea virus (BVDV) induced by DNA vaccination in calves

El-Attar

Thomas

Luke

et al. 2015

Vaccine

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“…The E2 protein is the most abundant surface protein of BVDV and contains the major BVDV-neutralizing antigenic site. It has been extensively utilized in the development of BVDV vaccines to induce BVDV-neutralizing antibodies ( 151 , 152 ). The novel MHC-II-targeted BVDV subunit vaccine has shown a rapid and sustained neutralizing antibody response in cattle compared to conventional vaccines ( 153 ).…”

Section: The Current Status Of Research On Major Bovine Vaccinesmentioning

confidence: 99%

The combination of vaccines and adjuvants to prevent the occurrence of high incidence of infectious diseases in bovine

Yao,

Zhang,

Yang

2023

Front. Vet. Sci.

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As the global population grows, the demand for beef and dairy products is also increasing. The cattle industry is facing tremendous pressures and challenges. The expanding cattle industry has led to an increased risk of disease in cattle. These diseases not only cause economic losses but also pose threats to public health and safety. Hence, ensuring the health of cattle is crucial. Vaccination is one of the most economical and effective methods of preventing bovine infectious diseases. However, there are fewer comprehensive reviews of bovine vaccines available. In addition, the variable nature of bovine infectious diseases will result in weakened or even ineffective immune protection from existing vaccines. This shows that it is crucial to improve overall awareness of bovine vaccines. Adjuvants, which are crucial constituents of vaccines, have a significant role in enhancing vaccine response. This review aims to present the latest advances in bovine vaccines mainly including types of bovine vaccines, current status of development of commonly used vaccines, and vaccine adjuvants. In addition, this review highlights the main challenges and outstanding problems of bovine vaccines and adjuvants in the field of research and applications. This review provides a theoretical and practical basis for the eradication of global bovine infectious diseases.

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Roles of bta-miR-29b promoter regions DNA methylation in regulating miR-29b expression and bovine viral diarrhea virus NADL replication in MDBK cells

Shi

Chen

2016

Arch Virol

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MicroRNAs (miRNAs) are an important class of small, non-coding RNAs that control target genes expression by degradation of target mRNAs or by inhibiting protein translation in many biological processes and cellular pathways. In a previous study, we found that miR-29b interfered with bovine viral diarrhea virus (BVDV) replication. However, the mechanisms of regulation of miR-29b expression are not well known. DNA methylation is an important epigenetic mechanism for silencing gene transcription, and plays an important role in promoter choice, protein expression, and regulation of miRNAs expression. In this study, we focused on the roles of DNA methylation of miR-29b promoter in regulating miR-29b expression and investigated the effects of DNA (cytosine-5) methyltransferase 1 (DNMT1) knockdown on miR-29b expression and BVDV (strain NADL) replication. Our results showed that methylation levels of miR-29b promoter were significantly decreased in BVDV NADL-infected MDBK cells. Furthermore, DNMT1 silencing significantly decreased the methylation levels of miR-29b promoter, up-regulated miR-29b expression and inhibited BVDV NADL replication, which supports the important roles of DNA methylation in regulating miRNA expression and further proves an evidence for our previous views.

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Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Cited by 7 publications

References 60 publications

Enhanced neutralising antibody response to bovine viral diarrhoea virus (BVDV) induced by DNA vaccination in calves

Enhanced neutralising antibody response to bovine viral diarrhoea virus (BVDV) induced by DNA vaccination in calves

The combination of vaccines and adjuvants to prevent the occurrence of high incidence of infectious diseases in bovine

Roles of bta-miR-29b promoter regions DNA methylation in regulating miR-29b expression and bovine viral diarrhea virus NADL replication in MDBK cells

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