Presence of bile acids in human follicular fluid and their relation with embryo development in modified natural cycle IVF

Nagy, Roland; Montfoort, van; Dikkers, Arne; Echten-Arends, Jannie van; Homminga, Irene; Land, Jolande; Hoek, Annemieke; Tietge, Uwe J. F.

doi:10.1093/humrep/dev034

Cited by 30 publications

(35 citation statements)

References 21 publications

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“…Smith et al () detected higher concentrations of BAs in human FF than in serum collected 30 min after the FF collection. In addition, Nagy et al () reported higher values of BAs in FF than in serum. In the present study concentrations in blood either collected during the physiological oestrus cycle or the OPU sessions compared to those in the FF were similar or slightly higher (Figure ).…”

Section: Discussionmentioning

confidence: 96%

“…BAs are usually known to be involved in physiological processes like solubilization of dietary lipids. But recently published data suggested a relation between BAs and reproduction in humans (Nagy et al, ; Smith et al, ) and cattle (Sanchez et al, ; Sanchez‐Guijo, Blaschka, et al, ), which opened a new field of research.…”

Section: Discussionmentioning

confidence: 99%

“…They detected a significant higher concentration of BAs in FF compared to serum and postulated BA production via follicular cells. In humans, Nagy et al () also determined a significant higher concentration of BAs in the fluid of follicles than in serum. Moreover, they associated individual follicular BA subspecies with embryo quality.…”

Section: Introductionmentioning

confidence: 97%

“…BAs are present in several body fluids like bile, blood and urine. Recently, BAs were also detected in follicular fluid (FF) of humans (Nagy et al, ; Smith et al, ) and cattle (Sanchez et al, ; Sanchez‐Guijo, Blaschka, Hartmann, Wrenzycki, & Wudy, ). Smith et al () determined the total BA concentration in human FF depending on an hCG (human chorionic gonadotropin) treatment.…”

Section: Introductionmentioning

confidence: 99%

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Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Blaschka

Sánchez‐Guijo

Wudy

et al. 2019

Veterinary Medicine & Sci

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The composition of follicular fluid (FF) has an impact on the developmental capacity of the oocyte and the resulting embryo. FF is composed of blood plasma constituents which cross the blood follicular barrier and the secretory components of granulosa and theca cells. Moreover, it has been shown recently that follicular cells have the ability to synthesize bile acids (BAs). BAs are present in several fluids of mammals especially in bile, blood and urine. FF is an essential impacting factor on the oocyte quality and therefore resulting embryos. To achieve a better understanding of this subject, the presence and concentration of BAs were measured in fluid collected from bovine follicles, categorized according to their size, throughout two entire oestrus cycles and compared to those in blood and urine. The body fluids were collected during the same examination procedure and in total samples from four heifers were obtained. A broad spectrum of 11 BA derivatives was measured applying liquid chromatography–tandem mass spectrometry (LC‐MS/MS). The simultaneous and direct quantification of BAs in different body fluids of cattle are reported. Within the follicular fluid, blood and urine, cholic acid and glycocholic acid are the dominant BA subspecies irrespective of the oestrus cycle stage. Moreover, BA concentrations in blood compared to those in the FF were similar. For the first time these results clearly highlight the presence of different BA subspecies in FF, blood and urine during the oestrus cycle in cattle.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Blaschka

Sánchez‐Guijo

Wudy

et al. 2019

Veterinary Medicine & Sci

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“…Plasma bile acid species were quantified by liquid chromatography-mass spectrometry [12]. To 25 μl of plasma, we added a mixture of internal standards (isotopically labeled bile acids).…”

Section: Methodsmentioning

confidence: 99%

Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet

Ronda

Heijning

Bruin

et al. 2020

Preprint

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Mouse models are frequently used to study mechanisms of human diseases. Recently, we observed a spontaneous bimodal variation in liver weight in C57BL/6JOlaHsd mice fed a semisynthetic diet. We now characterized the spontaneous variation in liver weight and its relationship with parameters of hepatic lipid and bile acid (BA) metabolism. In male C57BL/6JOlaHsd mice fed AIN-93G from birth to postnatal day (PN)70, we measured plasma BA, lipids, Very low-density lipoprotein (VLDL)-triglyceride (TG) secretion, and hepatic mRNA expression patterns. Mice were sacrificed at PN21, PN42, PN63 and PN70. Liver weight distribution was bimodal at PN70. Mice could be subdivided into two nonoverlapping groups based on liver weight: 0.6 SD 0.1 g (approximately one-third of mice, small liver; SL), and 1.0 SD 0.1 g (normal liver; NL; p<0.05). Liver histology showed a higher steatosis grade, inflammation score, more mitotic figures and more fibrosis in the SL versus the NL group. Plasma BA concentration was 14-fold higher in SL (p<0.001). VLDL-TG secretion rate was lower in SL mice, both absolutely (−66%, p<0.001) and upon correction for liver weight (−44%, p<0.001). Mice that would later have the SL-phenotype showed lower food efficiency ratios during PN21-28, suggesting the cause of the SL phenotype is present at weaning (PN21). Our data show that approximately one-third of C57BL/6JOlaHsd mice fed semisynthetic diet develop spontaneous liver disease with aberrant histology and parameters of hepatic lipid, bile acid and lipoprotein metabolism. Study designs involving this mouse strain on semisynthetic diets need to take the SL phenotype into account. Plasma lipids may serve as markers for the identification of the SL phenotype.

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Metabolomics for improving pregnancy outcomes in women undergoing assisted reproductive technologies

Siristatidis

Sertedaki

Vaidakis³

et al. 2017

Cochrane Database of Systematic Reviews

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According to current trials in women undergoing ART, there is insufficient evidence to show that metabolomic assessment of embryos before implantation has any meaningful effect on rates of live birth, ongoing pregnancy, or miscarriage rates. The existing evidence varied from very low to low-quality. Data on adverse events were sparse, so we could not reach conclusions on these. At the moment, there is no evidence to support or refute the use of this technique for subfertile women undergoing ART. Robust evidence is needed from further RCTs, which study the effects on live birth and miscarriage rates for the metabolomic assessment of embryo viability. Well designed and executed trials are also needed to study the effects on oocyte quality and endometrial receptivity, since none are currently available.

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Presence of bile acids in human follicular fluid and their relation with embryo development in modified natural cycle IVF

Abstract: This work was supported by a grant from the Netherlands Organisation for Scientific Research (VIDI Grant 917-56-358 to U.J.F.T.). No competing interests are reported.

Cited by 30 publications

References 21 publications

Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet

Metabolomics for improving pregnancy outcomes in women undergoing assisted reproductive technologies

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