Presence of an enamel matrix protein derivative on human teeth following periodontal surgery

Abstract

The aim of the study was to investigate immunohistochemically the expression of matrix molecules associated with periodontal tissues reformed after regenerative periodontal treatment. Chronic intrabony defects were treated with guided tissue regeneration, enamel matrix proteins, the combination of both, or access flap surgery. Five months after healing, the animals were killed, and the healed periodontal tissues were evaluated immunohistochemically by means of polyclonal antibodies against osteopontin, collagen I, and collagen III. The intact (nontreated) parts of the periodontium served as controls. As a general observation, the staining for all investigated matrix molecules appeared to be stronger within the regenerated tissues than in the intact ones. The results failed to reveal any differences in terms of staining intensity or distribution pattern of investigated matrix molecules between the four different treatments. Osteopontin expression was most intense at the border near the newly formed cementum and bone. In the regenerated periodontium, collagens I and III were localized throughout the entire periodontal ligament connective tissue. In the regenerated periodontal ligament, collagen III displayed more intense staining than collagen I. The present results suggest that: (1) even after a 5-month period following surgical periodontal therapy, extracellular matrix molecules associated with wound healing and/or remodelling are more strongly expressed in regenerated than in intact tissues and (2) once an environment for periodontal regeneration has been created, the expression of extracellular matrix molecules associated with the healing process seems to display the same pattern, irrespective of treatment modality.

Antibacterial effect of an enamel matrix protein derivative on in vivo dental biofilm vitality

“…Furthermore, recent data provide evidence that, once precipitated, EMD can be detected on treated root surfaces for at least 4 weeks [27]. Moreover, in vitro data have shown that, once precipitated onto a hard surface, EMD may inhibit the growth of certain gram-negative bacteria [31].…”

The Use of Enamel Matrix Derivative in the Treatment of Periodontal Defects: a Literature Review and Meta-analysis

“…Immunohistochemical analysis demonstrated that EMD was still present for 4 wks after its application on extracted rat molars that were transplanted to the abdominal wall (Hamamoto et al, 2002). In humans, it was demonstrated, by histological and immunochemical methods, that EMD is present on treated root surfaces for up to 4 wks following application during periodontal surgery (Sculean et al, 2002a).…”

“…Crit Rev Oral Biol Med 15(6): 382-402 (2004) demonstrated, by histological and immunochemical methods, that EMD is present on treated root surfaces for up to 4 wks following application during periodontal surgery (Sculean et al, 2002a).…”

The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization

“…EMP has been shown to facilitate regeneration of the periodontium although its mechanism of action is not fully elucidated [1][2][3]. Recent clinical studies have demonstrated that the EMP matrix supports recolonization with mesenchymal cells [4]. In vitro, periodontal ligament cells derived from the mesenchyme, when cultured on EMP, increased their attachment rate, growth rate and metabolism, and subsequently released several growth factors and extracellular matrix (ECM) molecules including fibronectin at elevated levels into the medium [5][6][7][8][9][10][11].…”