Presence and Transfer of Antimicrobial Resistance Determinants in Escherichia coli in Pigs, Pork, and Humans in Thailand and Lao PDR Border Provinces

Pungpian, Chanika; Sinwat, Nuananong; Prathan, Rangsiya; Chuanchuen, Rungtip

doi:10.1089/mdr.2019.0438

Cited by 24 publications

(29 citation statements)

References 40 publications

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“…Similarly, a high incidence of multidrug-resistant foodborne pathogens in fresh vegetables retailed has been recently reported in South Africa (29). In Thailand, high rates of MDR in E. coli including ESBL types have been observed in humans, livestock, and environmental sources (6)(7)(8)(9). This work therefore highlights the occurrence of MDR-ESBL-producing E. coli in vegetables.…”

Section: Accepted Manuscriptmentioning

confidence: 62%

“…ESBL-producing E. coli are among the most common Gram-negative bacteria isolated from the clinical samples in Thailand (5). There are several studies on ESBL-producing E. coli from livestock, food, and water samples in Thailand (6)(7)(8)(9)(10). However, the epidemiology and characterization of ESBL-producing E. coli from vegetables is limited (11).…”

Section: Introductionmentioning

confidence: 99%

“…The CTX-M ESBL family is now comprised of over 50 different types which can be divided into 5 groups based on their amino acid identities: CTX-M1, CTX-M2, CTX-M8, CTX-M9, and CTX-M25 (12). The CTX-M-55 belong to CTX-M-1 group and CTX-M-14, a member of CTX-M-9 group, are predominant findings in Thailand among clinical isolates (7,13) including Thai and swine carriers (6,9). Most of the ESBL-producing E. coli can be found in food along the food production chain in selected areas of Thailand (10).…”

Section: Introductionmentioning

confidence: 99%

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The Emergence of CTX-M-55 in Extended-Sectrum β-Lactamase-Producing Escherichia coli from Vegetables Sold in Local Markets of Northern Thailand

2022

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Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are spreading worldwide and pose a public health issue. An assessment of their presence in the environment and in food chain products would clarify the pathway of this foodborne transmission. Here, we investigated the prevalence of ESBL-producing E. coli in fresh vegetables purchased from fresh markets in Chiang Rai, Thailand. Overall, 8.8% of the samples collected contained ESBL-producing E. coli, of which 81.3% were multidrug-resistant. All isolates carried the blaCTX-M-55 gene, and 10 isolates contained the ISEcp1 gene. One E. coli strain carried blaCTX-M-55 coexisting with blaTEM-1. Thirteen different sequence types (ST48,

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Section: Accepted Manuscriptmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Emergence of CTX-M-55 in Extended-Sectrum β-Lactamase-Producing Escherichia coli from Vegetables Sold in Local Markets of Northern Thailand

2022

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“…Integrase genes were present in 13 of the livestock isolates as well as in the one from the magpie. Only class 1 integrons were detected, which are known to be the most common in enteric bacteria and are highly prevalent among isolates of pigs, cattle and wild birds [ 21 , 74 , 75 ]. In our study, intI1 positive E. coli strains co-harbored bla CTX-M-1/15 and different combinations of resistance determinants for at least three classes of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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The prevalence of multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The present study aimed to provide an overview of the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli (E. coli) isolates of livestock and wild bird origin in Greece. Nineteen phenotypically confirmed ESBL-producing E. coli strains isolated from fecal samples of cattle (n = 7), pigs (n = 11) and a Eurasian magpie that presented resistance to at least one class of non β-lactam antibiotics, were selected and genotypically characterized. A DNA-microarray based assay was used, which allows the detection of various genes associated with antimicrobial resistance. All isolates harbored blaCTX-M-1/15, while blaTEM was co-detected in 13 of them. The AmpC gene blaMIR was additionally detected in one strain. Resistance genes were also reported for aminoglycosides in all 19 isolates, for quinolones in 6, for sulfonamides in 17, for trimethoprim in 14, and for macrolides in 8. The intI1 and/or tnpISEcp1 genes, associated with mobile genetic elements, were identified in all but two isolates. This report describes the first detection of multidrug resistance genes among ESBL-producing E. coli strains retrieved from feces of cattle, pigs, and a wild bird in Greece, underlining their dissemination in diverse ecosystems and emphasizing the need for a One-Health approach when addressing the issue of antimicrobial resistance.

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“…A total of 988 E. coli and 631 Salmonella isolates from pigs (n = 377 and 139), pig carcasses (n = 328 and 174), and pork (n = 283 and 318) were included. They were isolated previously as part of AMR epidemiological studies in food animals and products in Southeast Asia between 2014 and 2018 [ 12 13 14 15 ]. The E. coli and Salmonella isolates were obtained from Thailand (n = 508 and 276), Lao PDR (n = 351 and 237), and Cambodia (n = 129 and 118) ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Colistin resistance and plasmid-mediatedmcrgenes inEscherichia coliandSalmonellaisolated from pigs, pig carcass and pork in Thailand, Lao PDR and Cambodia border provinces

et al. 2021

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Background Colistin and carbapenem-resistant bacteria have emerged and become a serious public health concern, but their epidemiological data is still limited. Objectives This study examined colistin and carbapenem resistance in Escherichia coli and Salmonella from pigs, pig carcasses, and pork in Thailand, Lao PDR, and Cambodia border provinces. Methods The phenotypic and genotypic resistance to colistin and meropenem was determined in E. coli and Salmonella obtained from pigs, pig carcasses, and pork (n = 1,619). A conjugative experiment was performed in all isolates carrying the mcr gene (s) (n = 68). The plasmid replicon type was determined in the isolates carrying a conjugative plasmid with mcr by PCR-based replicon typing (n = 7). The genetic relatedness of mcr -positive Salmonella (n = 11) was investigated by multi-locus sequence typing. Results Colistin resistance was more common in E. coli (8%) than Salmonella (1%). The highest resistance rate was found in E. coli (17.8%) and Salmonella (1.7%) from Cambodia. Colistin-resistance genes, mcr-1 , mcr-3 , and mcr-5 , were identified, of which mcr-1 and mcr-3 were predominant in E. coli (5.8%) and Salmonella (1.7%), respectively. The mcr-5 gene was observed in E. coli from pork in Cambodia. Two colistin-susceptible pig isolates from Thailand carried both mcr-1 and mcr-3 . Seven E. coli and Salmonella isolates contained mcr-1 or mcr-3 associated with the IncF and IncI plasmids. The mcr -positive Salmonella from Thailand and Cambodia were categorized into two clusters with 94%–97% similarity. None of these clusters was meropenem resistant. Conclusions Colistin-resistant E. coli and Salmonella were distributed in pigs, pig carcasses, and pork in the border areas. Undivided-One Health collaboration is needed to address the issue.

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Presence and Transfer of Antimicrobial Resistance Determinants in Escherichia coli in Pigs, Pork, and Humans in Thailand and Lao PDR Border Provinces

Cited by 24 publications

References 40 publications

The Emergence of CTX-M-55 in Extended-Sectrum β-Lactamase-Producing <i>Escherichia coli</i> from Vegetables Sold in Local Markets of Northern Thailand

The Emergence of CTX-M-55 in Extended-Sectrum β-Lactamase-Producing <i>Escherichia coli</i> from Vegetables Sold in Local Markets of Northern Thailand

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Colistin resistance and plasmid-mediatedmcrgenes inEscherichia coliandSalmonellaisolated from pigs, pig carcass and pork in Thailand, Lao PDR and Cambodia border provinces

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