Preprotein Translocation through the Sec Translocon in Bacteria

Maillard, Antoine P.; Chan, Kenneth Ken-Yin; Duong, Franck

doi:10.1007/0-387-30871-7_2

Cited by 2 publications

(2 citation statements)

References 105 publications

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“…In bacteria, the Sec complex is composed of three membrane proteins (SecYEG) which conduct polypeptides through or into the cytosolic membrane (for recent reviews, see Rapoport et al , 2004; Veenendaal et al , 2004). Depending on the type of substrate, the Sec complex cooperates with either the ribosome or the ATPase SecA to push the protein by co‐ or post‐translational mechanisms, respectively (Dalbey and Chen, 2004; Luirink and Sinning, 2004; Maillard et al , 2005).…”

Section: Introductionmentioning

confidence: 99%

Investigating the SecY plug movement at the SecYEG translocation channel

Tam

Maillard

Chan

et al. 2005

EMBO J

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139

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Protein translocation occurs across the energy-conserving bacterial membrane at the SecYEG channel. The crystal structure of the channel has revealed a possible mechanism for gating and opening. This study evaluates the plug hypothesis using cysteine crosslink experiments in combination with various allelic forms of the Sec complex. The results demonstrate that the SecY plug domain moves away from the center of the channel toward SecE during polypeptide translocation, and further show that the translocation-enhancing prlA3 mutation and SecG subunit change the properties of channel gating. Locking the plug in the open state preactivates the Sec complex, and a super-active translocase can be created when combined with the prlA4 mutation located in the pore of the channel. Dimerization of the Sec complex, which is essential for translocase activity, relocates the plug toward the open position. We propose that oligomerization may result in SecYEG cooperative interactions important to prime the translocon function.

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Section: Introductionmentioning

confidence: 99%

Investigating the SecY plug movement at the SecYEG translocation channel

Tam

Maillard

Chan

et al. 2005

EMBO J

Self Cite

121

139

View full text Add to dashboard Cite

show abstract

“…The effluent saw an upregulation of secG, sec-C, and secY while the chlorinated effluent saw increases in secF and secE (Tables S4 and S5). The main translocon channel is composed of proteins SecY, SecE and SecG [52]. While secY, secE and secG were not upregulated in both effluents, secG and secY gene were upregulated in the effluent and by the time A. junii transitions to the chlorination chamber, these proteins would already be in abundance.…”

Section: Discussionmentioning

confidence: 93%

An Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment

Jumat

Haroon

Aljassim

et al. 2018

Water

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A membrane bioreactor (MBR)-based wastewater treatment plant (WWTP) in Saudi Arabia is assessed over a five-month period in 2015 and once in 2017 for bacterial diversity and transcriptional activity using metagenomics, metatranscriptomics and real time quantitative polymerase chain reaction (RT-qPCR). Acinetobacter spp. are shown to be enriched in the chlorinated effluent. Members of the Acinetobacter genus are the most abundant in the effluent and chlorinated effluent. At the species level, Acinetobacter junii have higher relative abundances post MBR and chlorination. RNA-seq analysis show that, in A. junii, 288 genes and 378 genes are significantly upregulated in the effluent and chlorinated effluent, respectively, with 98 genes being upregulated in both. RT-qPCR of samples in 2015 and 2017 confirm the upregulation observed in RNA-seq. Analysis of the 98 genes show that majority of the upregulated genes are involved in cellular repair and metabolism followed by resistance, virulence, and signaling. Additionally, two different subpopulations of A. junii are observed in the effluent and chlorinated effluent. The upregulation of cellular repair and metabolism genes, and the formation of different subpopulations of A. junii in both effluents provide insights into the mechanisms employed by A. junii to persist in the conditions of a WWTP.

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Preprotein Translocation through the Sec Translocon in Bacteria

Cited by 2 publications

References 105 publications

Investigating the SecY plug movement at the SecYEG translocation channel

Investigating the SecY plug movement at the SecYEG translocation channel

An Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment

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