Preponderance of mixed infection of Cucumber mosaic virus and Candidatus Phytoplasma australasia on brinjal in India

Abirami, R.; Manoranjitham, S. K.; Mohankumar, S.; Karthikeyan, G.

doi:10.1016/j.micpath.2022.105596

“…There have been many reports on the use of TEM for virus observation. For instance, cucumber mosaic virus, tobacco rattle virus, ranunculus mild mosaic virus, zucchini tigre mosaic virus, and staphylococcus aureus were observed in different host [23][24][25][26][27][28]. In this study, the Sphular virus particles were observed under TEM by negative staining for the samples and the diameter ranged from 80 to 120nm.…”

Section: Discussionmentioning

confidence: 60%

Untitled

2023

MCDA

0

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Tomato Spotted Wilt orthotospovirus (TSWV) is a member of the Orthotospovirus genus. It has extensive host and causes serious disease in many crops. In May 2021, virus-like symptoms such as chlorosis, mottling, leaf yellowing and necrosis were observed on Polygonum multiflorum (hereinafter as P. multiflorum) leaves in Kunming, Yunnan Province, China. Spherical viral particles with a diameter of 80-120nm were observed by TEM.RT-PCR and DNA sequencing analysis were performed, and the results showed that the sequence of N gene for this virus shared 99.20% identity with TSWV isolate from Shandong isolate (acc. no. MN861982.1).The obtained 777-bp consensus sequence named TSWN-N was deposited in GenBank (acc. no. OP800910).Phylogenetic tree analyses suggested that TSWV obtained from P. multiflorum plant samples clustered together into a large clade from Korea. Koch's postulate was performed, and the virus was re-amplied from the inoculated plants. This is the first report of TSWV on P. multiflorum in China and provides early warning of virus disease on medicinal materials.

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“…There have been many reports on the use of TEM for virus observation. For instance, cucumber mosaic virus, tobacco rattle virus, ranunculus mild mosaic virus, zucchini tigre mosaic virus, and staphylococcus aureus were observed in different host [23][24][25][26][27][28]. In this study, the Sphular virus particles were observed under TEM by negative staining for the samples and the diameter ranged from 80 to 120nm.…”

Section: Discussionmentioning

confidence: 60%

First Report of Tomato Spotted Wilt Orthotospovirus Infecting Polygonum Multiflorum in China

Zhao

2023

MCDA

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Tomato Spotted Wilt orthotospovirus (TSWV) is a member of the Orthotospovirus genus. It has extensive host and causes serious disease in many crops. In May 2021, virus-like symptoms such as chlorosis, mottling, leaf yellowing and necrosis were observed on Polygonum multiflorum (hereinafter as P. multiflorum) leaves in Kunming, Yunnan Province, China. Spherical viral particles with a diameter of 80-120nm were observed by TEM.RT-PCR and DNA sequencing analysis were performed, and the results showed that the sequence of N gene for this virus shared 99.20% identity with TSWV isolate from Shandong isolate (acc. no. MN861982.1).The obtained 777-bp consensus sequence named TSWN-N was deposited in GenBank (acc. no. OP800910).Phylogenetic tree analyses suggested that TSWV obtained from P. multiflorum plant samples clustered together into a large clade from Korea. Koch's postulate was performed, and the virus was re-amplied from the inoculated plants. This is the first report of TSWV on P. multiflorum in China and provides early warning of virus disease on medicinal materials.

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“…Even though the production of global Chilies has significantly increased in the past 5 years (2018–2022), the crops still impacted by pests and pathogens, including whiteflies ( Hussain et al, 2022 ), thrips ( Panyasiri et al, 2022 ), broad mites ( Latha and Hunumanthraya, 2018 ), aphids ( Xiao et al, 2020 ), fruit flies ( Syamsudin et al, 2022 ), Colletotrichum ( Mongkolporn and Taylor, 2018 ), Phytophthora ( Mohammadbagheri et al, 2022 ), Fusarium ( Bashir et al, 2018 ), Xanthomonas ( Utami et al, 2022 ), and viruses ( Abirami et al, 2022 ). To address these issues, chemical pesticides have been widely used, leading to the development of pesticide resistance among pests and pathogens ( Hawkins et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%