Preparative Separation of Alkaloids from Green Tea by pH‐Zone‐Refining Countercurrent Chromatography

Yuan, Li; Chen, X. X.; Ai, Ping; Qi, Shihua; Li, B. F.; Wang, D.; Miao, Li; Liu, Z. F.

doi:10.1081/jlc-120027106

Cited by 8 publications

(1 citation statement)

References 6 publications

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“…A broad variety of anthocyanins have been isolated with LLC from red wine, elderberry and blood orange juice, blackberries, purple corn, black rice, grape seeds, pine bark, cinnamon bark, cocoa seeds, and eggplant (Degenhardt et al, 2001; Fan, Li, et al, 2020; Hillebrand et al, 2004; Jeon et al, 2015; Phansalkar et al, 2018; Schwarz et al, 2003). The polyphenolic profile (together with other classes of compounds) in tea or coffee extracts has also been explored with the use of LLC (Fan et al, 2022; Si et al, 2006; Stodt et al, 2015; Yanagida et al, 2006; Yuan et al, 2004). LLC has also been applied in separating polyphenols from red wine and rum aged in oak barrels (Fan et al, 2015; Regalado et al, 2011).…”

Section: Phenolic Compoundsmentioning

confidence: 99%

Liquid–liquid chromatography as a promising technology in the separation of food compounds

Kulinowski

Luca

Skalicka‐Woźniak

2023

eFood

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Section: Phenolic Compoundsmentioning

confidence: 99%

Liquid–liquid chromatography as a promising technology in the separation of food compounds

Kulinowski

Luca

Skalicka‐Woźniak

2023

eFood

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Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pH‐zone‐refining counter‐current chromatography

Wang

Liu

Geng

et al. 2010

J of Separation Science

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Preparative separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn has been successfully performed for the first time using pH-zone-refining counter-current chromatography. Two kinds of two-phase solvent systems were applied to preparative CCC isolation. The first was the system composed of MtBE-water (1:1, v/v), 10 mM triethylamine in organic stationary phase and 5 mM HCl in aqueous mobile phase, which could only separate neferine partly from the crude extract. The second was the system composed of n-hexane-ethyl acetate-methanol-water (5:5:2:8, v/v), 10 mM triethylamine in organic stationary phase and 5 mM HCl in aqueous mobile phase, which was the optimum for large-scale CCC isolation. 2.5 g of the crude alkaloid was purified in one-step separation of 7 h, yielding 151 mg of liensinine, 118 mg of isoliensinine and 572 mg of neferine with the purities of 93.0, 95.1 and 97.0%, respectively.

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Separation of alkaloids from herbs using high‐speed counter‐current chromatography

Fang¹,

Liu²,

Yang³

et al. 2011

J of Separation Science

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Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatography with a support-free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH-zone-refining counter-current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH-zone-refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH-zone-refining CCC are also summarized.

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Preparative Separation of Alkaloids from Green Tea by pH‐Zone‐Refining Countercurrent Chromatography

Cited by 8 publications

References 6 publications

Liquid–liquid chromatography as a promising technology in the separation of food compounds

Liquid–liquid chromatography as a promising technology in the separation of food compounds

Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pH‐zone‐refining counter‐current chromatography

Separation of alkaloids from herbs using high‐speed counter‐current chromatography

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