Preparative purification of bromelain (EC 3.4.22.33) from pineapple fruit by high-speed counter-current chromatography using a reverse-micelle solvent system

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

“…Several chromatographic techniques such as ion exchange chromatography (Hung et al 2002;Arumugam and Ponnusami 2013;Iara et al 2013;Swaroop and Viswanathan 2013), high-speed counter-current chromatography (HSCCC) (Yin et al 2011), affinity chromatography, gel filtration chromatography and capillary electrochromatography (Cheng and Huang 2004;Babu et al 2008;Chen et al 2008) have been employed for the purification of bromelain. All these techniques possess different specifications thus result in different purification efficiency and recovery (Yin et al 2011). Amongst these, ion exchange chromatography (IEX) is extensivelyemployed for bromelain separation (Devi and Sowmiya, 2014).…”

“…10 fold purification of bromelain was achieved using cation exchange chromatography (Devakate et al 2009). High-speed counter-current chromatography (HSCCC) has also been used to purify bromelain which generated 3.01g bromelain from 5.00g crude extract (Yin et al 2011). Immobilized metal affinity membrane (IMAM) has been employed to purify bromelain which resulted in 15.4 fold purification factor with 94.6% recovery .…”

“…Efforts are being made by researchers to achieve highly purified bromelain in less steps and low cost. Modern strategies, such as membrane filtration (Lopes et al 2009), reverse micellar systems (Upadhyay et al 2010;Kumar et al 2011), aqueous two phase extraction (Coelho et al 2013;Novaeset al 2013) and chromatographic techniques (Yin et al 2011) have shown promise in this regard. Pineapple plant also contains minor quantities of other proteinases like ananain and comosain (Nadzirah et al 2013) but bromelain is regarded as a primary and extensively investigated component (Amini et al 2016).…”

Bromelain: an overview of industrial application and purification strategies

“…The movement of protein inside and outside the micelle can be induced by manipulating the pH, the ionic strength of aqueous phase, and the surfactant concentration (Wheelwright 1991). Several reports on the application of RME for separation and purification of bromelain from pineapple fruit (Yin et al 2011), juice (Fileti et al 2009), and wastes (Chaurasiya and Umesh Hebbar 2013;Hebbar et al 2012;Umesh Hebbar et al 2008) are available. Yin et al (2011) and Umesh Hebbar et al (2008) have demonstrated that the forward extraction efficiency, purification fold, and specific activity of bromelain are optimal at pH 7.0-8.0.…”

“…Several reports on the application of RME for separation and purification of bromelain from pineapple fruit (Yin et al 2011), juice (Fileti et al 2009), and wastes (Chaurasiya and Umesh Hebbar 2013;Hebbar et al 2012;Umesh Hebbar et al 2008) are available. Yin et al (2011) and Umesh Hebbar et al (2008) have demonstrated that the forward extraction efficiency, purification fold, and specific activity of bromelain are optimal at pH 7.0-8.0. The proteins transfer from the organic solvent to the reverse micelle if the pH value of the aqueous phase is higher than the isoelectric point of bromelain (4.6).…”