Preparative medium pressure liquid chromatographic (MPLC) and semipreparative HPLC separation of furocoumarin isomers

Zogg, G.; Nyiredy, Sz.; Sticher, Otto

doi:10.1007/bf02258984

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…The results in the study indicated that several phenolic compounds are potent inhibitors of high K + -evoked 45 Ca 2+ entry, showing effects comparable to the clinically used compound, verapamil. In addition, they showed an inhibitory activity on calcium entry similar to that of other classes of natural calcium channel antagonists, e.g., furanocoumarins [110].…”

Section: *Hl-cells Pre-incubated For 24 H With the Investigated Compoundmentioning

confidence: 77%

“…The goal for laboratory scale preparative separation is to separate and isolate all interesting compounds with the highest purity possible. In most cases the column is then only lightly overloaded [45][46][47].…”

Section: Scale Upmentioning

confidence: 99%

“…The calcium antagonistic activity of 51 extracts was investigated by assaying the inhibition of depolarizationinduced 45 Ca 2+ uptake in the rat pituitary, GH 4 C 1 , cells that possess L-type voltage-operated calcium channels (VOCC's) [108,109]. The most effective plants were Humulus lupulus, Epilobium angustifolium, Lythrum salicaria, and Lysimachia vulgaris.…”

Section: *Hl-cells Pre-incubated For 24 H With the Investigated Compoundmentioning

confidence: 99%

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Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

279

187

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Natural derived or originated compounds still play a major role as drugs, and as lead structures for the development of synthetic molecules. About 50% of the drugs introduced to the market during the last 20 years are derived directly or indirectly from small biogenic molecules. In the future, natural products will continue to play a major role as active substances, model molecules for the discovery and validation of drug targets. A multidisciplinary approach to drug discovery involving the generation of truly novel molecular diversity from natural product sources, combined with total and combinatorial synthetic methodologies provides the best solution to increase the productivity in drug discovery and development. Screening for new drugs in plants implies the screening of extracts for the presence of novel compounds and an investigation of their biological activities. It is currently estimated that approximately 420,000 plant species exist in nature. For the purpose of lead discovery, or for the scientific validation of a traditional medicinal plant or a phytopharmaceutical, active principals in complex matrices need to be identified. Therefore, the interfacing of biological and chemical assessment becomes the critical issue. Drug discovery from plants can be guided by epidemiologic studies facilitated with computer assisted HPLC microfractionation and microplate technology. Epidemiologic studies have shown that high dietary flavonoid intake may be associated with decreased risk for cardiovascular disease. Chlamydia pneumoniae is a common human pathogen and epidemiological and clinical studies have shown a connection between chronic C. pneumoniae infection, atherosclerosis and the risk of myocardial infarction. We will present here the detection of natural compounds active against C. pneumoniae as an example.

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Section: *Hl-cells Pre-incubated For 24 H With the Investigated Compoundmentioning

confidence: 77%

Section: Scale Upmentioning

confidence: 99%

Section: *Hl-cells Pre-incubated For 24 H With the Investigated Compoundmentioning

confidence: 99%

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Natural Products in the Process of Finding New Drug Candidates

Vuorela¹,

Leinonen²,

Saikku³

et al. 2004

CMC

279

187

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“…Zogg et al (1989a) have described the dry filling ofLabomatic glass columns and the transposition of analytical OPLC conditions to MPLC for the separation of furanocoumarins. In the preparation of these phases, attention is paid to careful end-capping of the treated silica gel, to give a fully hydrophobic surface.…”

Section: Labomatic Installationsmentioning

confidence: 99%

Preparative Pressure Liquid Chromatography

Hostettmann

Marston

Hostettmann

1998

Preparative Chromatography Techniques

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“…This phenomenon, sometimes very troublesome for the OPLC user, has been studied by and possibilities for the elimination of the disturbing zone have been proposed. Zogg et al (1989b) evaluated the potential use of analytical on-line OPLC as a pilot technique for medium pressure liquid chromatography (MPLC), and found a linear correlation between the k' data of analytical on-line OPLC and preparative MPLC. Thus, it should be possible to predict retention data for MPLC from an analytical OPLC separation.…”

Section: Planar Chromatograpfiymentioning

confidence: 99%

New planar chromatographic techniques in natural product analysis

Erdelmeier

Körig

1991

Phytochemical Analysis

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Planar chromatography (PLC) is gaining importance in the analysis of natural products. Sophisticated instrumentation has become available in this field. Major progress in planar chromatography has been achieved through the development of forced-flow techniques. Progress has also been made in the capillary-controlled flow techniques with the use of modified development chambers. A large variety of stationary phases is available and strategies to optimize mobile phase compositions in PLC have been developed. The detection and quantitation in PLC has improved through direct coupling of PLC with spectroscopic methods with and the use of modern, computerized scanning densitometers.

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Preparative medium pressure liquid chromatographic (MPLC) and semipreparative HPLC separation of furocoumarin isomers

Cited by 14 publications

References 14 publications

Natural Products in the Process of Finding New Drug Candidates

Natural Products in the Process of Finding New Drug Candidates

Preparative Pressure Liquid Chromatography

New planar chromatographic techniques in natural product analysis

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