Preparative Pressure Liquid Chromatography

Among catechin compounds abundant mainly in green tea, (2)epigallocatechin gallate (EGCG) has antioxidative and anticarcinogenic properties. Korean green tea (Bosung, Chonnam) was used as a feed sample. The separation was done by a C 18 reversedphase preparative column (22 £ 250 mm) with mobile phases of 0.1% acetic acid in water, acetonitrile, methanol, and ethyl acetate considered. The injection volume and the flow rate of mobile phase were fixed at 400 m L and 16 mL/min, respectively. To purify EGCG preparatively, the effluent was collected from the column outlet and concentrated, and analyzed by analytical column (3.9 £ 300 mm). From the experimental results, the optimum mobile phase condition for separating EGCG from Korean green tea was 0.1% acetic acid in water/acetonitrile/methanol/ethyl acetate (80/16/1/3 vol.%). In the experimental condition, 0.6 mg of EGCG was obtained purely. 1631

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Preparative Pressure Liquid Chromatography

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HPLC quantification of two isomeric triterpenic acids isolated from Mitracarpus scaber and antimicrobial activity on Dermatophilus congolensis

Preparative separation of EGCG from Korean green tea by high performance liquid chromatography

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