Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography

Abstract

Three anthraquinones-emodin, chrysophanol, and physcion-were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with nhexane-ethanol-water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by 1 H-NMR and 13 C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.

Dietary-flavonoid-rich flowers ofRumex nervosusVahl: Liquid chromatography with electrospray ionization tandem mass spectrometry profiling and in vitro anti-inflammatory effects

“…Furthermore, R. nervosus could share comparable biological activities (as stated above) to other Rumex species [17,[26][27][28]. The biological effects observed can, at least partly, be attributed to flavonoids [29][30][31]. To our knowledge, until now there has been no reported characterization of flavonoids from individual tissues of R. nervosus.…”

Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

“…Thus, this method has been widely used for separation and purification of various natural products [9,11,12,13,14,15,16]. However, there is no report on the use of HSCCC for the isolation and purification of sesquiterpenoid lactones from Eupatorium lindleyanum DC, thus we report herein an efficient method for the preparative isolation and purification of three sesquiterpenoid lactones (structures shown in Figure 1) from Eupatorium lindleyanum DC.…”

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

“…Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples. Because of these advantages, HPCCC is gaining popularity as a purification tool for natural products [9][10][11]13].…”

“…Current purification methods include the use of labor intensive and time-consuming columns with a variety of stationary phases and sequential solvent extraction [5][6][7][8]. High performance countercurrent chromatography (HPCCC) is a liquid-liquid chromatographic separation technique used for the isolation of natural compounds [9][10][11][12]. Compared with traditional liquid adsorption chromatography in which a solid phase is involved, HPCCC has numerous advantages such as no irreversible adsorption and complete recovery in samples.…”