Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Mbeunkui, Flaubert; Grace, Mary H.; Lila, Mary Ann

doi:10.1016/j.jchromb.2011.12.018

Cited by 10 publications

(11 citation statements)

References 34 publications

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“…The mass spectrometry analysis of purified compounds was performed as described previously [] with some modifications. The mass spectrometry conditions were as follows: the heat block and curved desolvation line were maintained at 200°C.…”

Section: Methodsmentioning

confidence: 99%

“…HPCCC in combination with high‐performance liquid chromatography with tandem mass spectrometry (HPLC‐MS n ) is a selective and proficient method for minor bioactive compounds isolation, identification and characterization. Our laboratory has successfully applied this technology to the isolation, identification, and characterization of antimalarial compounds purified from a variety of plants [].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of flavonols from blackcurrant by high‐performance counter‐current chromatography and electrospray ionization tandem mass spectrometry

Mbeunkui¹,

Grace²,

Yousef³

et al. 2012

J of Separation Science

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Blackcurrant is considered as a natural high-value food raw material and possesses a variety of therapeutic properties. The health benefits of blackcurrant have generally been credited to its high anthocyanin content; however, the therapeutic properties of other minor flavonoids constituents have not yet been investigated due the difficulties related to their isolation. Multiple steps of high-performance counter-current chromatography in combination with ESI tandem mass spectrometry (MS(n)) were successfully used for the preparative isolation of flavonols from blackcurrant extract, to study their electrospray ionization mass spectrometry fragmentation behavior. Seven flavonols, namely myricetin-3-O-rutinoside (145.5 mg), myricetin-3-O-hexoside (79.7 mg), myricetin-3-O-(6″-malonyl)-glucoside (17.4 mg), kaempferol-3-O-glucoside (20.5 mg), quercetin-3-O-rutinoside (55.1 mg), quercetin-3-O-hexoside (25.8 mg), and myricetin (129.1 mg) have been successfully isolated and their multistage MS(n) data were used for detailed structure characterization. The results of these experiments demonstrated that high-performance counter-current chromatography along with ESI-MS(n) is a sensitive, selective, and effective technology for isolation and characterization of minor constituents from a complex mixture.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of flavonols from blackcurrant by high‐performance counter‐current chromatography and electrospray ionization tandem mass spectrometry

Mbeunkui¹,

Grace²,

Yousef³

et al. 2012

J of Separation Science

Self Cite

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show abstract

“…According to the described experimental procedure in the experimental section, 5 major performed to confirm the geissospermine structure by comparison with available reported data [72]. The NMR ¹H and ¹³C chemicals shifts are shown in Table 7.…”

Section: Geissospermine Isolation By Cpc and Characterization By Nmr mentioning

confidence: 99%

Corrosion inhibition of C38 steel by alkaloids extract of Geissospermum laeve in 1M hydrochloric acid: Electrochemical and phytochemical studies

et al. 2015

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“…There are few studies on the separation and isolation of TIAs using CCC. They report on the separation of alkaloids from C. roseus (Renault et al, 1999), Strychnos guaianensis (Quetin-Leclercq et al, 1995), S. nux-vomica (Miao Cai, Xiang, An, & Ito, 1998), Hortia oreadica (Severino et al, 2009), Geissospermum vellosi (Mbeunkui, Grace, & Lila, 2012) and Tabernaemontana (van der Heijden et al, 1987), and T. catharinensis (Gonçalves, Curcino, Oliveira, & Braz-Filho, 2011).…”

Section: High-speed Counter-current Chromatographymentioning

confidence: 99%

New Methods of Analysis and Investigation of Terpenoid Indole Alkaloids

Goldhaber-Pasillas

Choi

Verpoorte

2013

New Light on Alkaloid Biosynthesis and Future Prospects

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Introduction 235 2. Extraction and Purification Methods 236 2.1 Solvent extraction 237 2.2 Ionic liquids 239 2.3 Molecular imprinted polymers 239 2.4 Supercritical CO 2 240 3. Analytical Methods 242 3.1 HPLC and hyphenated techniques 242 3.2 Ultra high-pressure LC-MS 243 3.3 Gas chromatography coupled to mass spectroscopy 248 3.4 High-speed counter-current chromatography 251 3.5 Capillary electrophoresis 253 3.6 Quantitative nuclear magnetic resonance 259 4. Applications in Fingerprint Analysis 260 4.1 NMR and LC-MS-based metabolic fingerprinting on TIAs 260 5. Conclusions 262 References 263

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Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

Cited by 10 publications

References 34 publications

Isolation and characterization of flavonols from blackcurrant by high‐performance counter‐current chromatography and electrospray ionization tandem mass spectrometry

Isolation and characterization of flavonols from blackcurrant by high‐performance counter‐current chromatography and electrospray ionization tandem mass spectrometry

Corrosion inhibition of C38 steel by alkaloids extract of Geissospermum laeve in 1M hydrochloric acid: Electrochemical and phytochemical studies

New Methods of Analysis and Investigation of Terpenoid Indole Alkaloids

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