Preparative isolation and purification of B-type fumonisins by using macroporous resin column and high-speed countercurrent chromatography

Hu, Jin; Lv, Hui; Hou, Mingxuan; Wang, Gang; Lee, Yin-Won; Shi, Jianrong; Gu, Zhenxin; Xu, Jianhong

doi:10.1080/19440049.2019.1678768

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…In this study, we reported that the modified strain ΔFaFlbA was prolific in NIV production (2.07 g kg −1 ) and 4-AcNIV (2.85 g kg −1 ). Preparative methods have been developed in our laboratory for other major Fusarium mycotoxins, including B-type fumonisin FB1, estrogenic zearalenone and DON (Hu et al, 2020;Wang et al, 2018Wang et al, , 2019. Brown long-grain rice culture of ΔFaFlbA served as a good starting resource for mass production and the development of preparation and purification methods for NIV and 4AcNIV.…”

Section: Discussionmentioning

confidence: 99%

“…Access to a liberal supply of pure NIV is of significant importance for toxicological studies, quantitative determination and screening for effective degrading micro-organisms. We have previously reported preparative purification of major Fusarium mycotoxins from fungal cultures (Hu et al, 2020;Wang et al, 2018Wang et al, , 2019. Inoculation with a mycotoxinproducing strain with high toxin productivity is the crucial first step to achieving a high yield.…”

Section: Introductionmentioning

confidence: 99%

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The FaFlbA mutant of Fusarium asiaticum is significantly increased in nivalenol production

Fang

Dong

Wang

et al. 2022

Journal of Applied Microbiology

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Aims: Cereals contaminated with type B trichothecene nivalenol (NIV) and its acetylated derivative 4-acetyl-nivalenol (4-AcNIV) are a global mycotoxicological problem threatening the health of humans and livestock. Toxicological studies, quantitative determinations and screening for biodegrading micro-organisms require massive amounts of pure toxins. However, the low yield from fungal cultures and high prices of NIV and 4-AcNIV limit research progress in these areas. This work aimed to select Fusarium asiaticum mutant strains with enhanced production of NIV and 4-AcNIV. Methods and Results:A total of 62 NIV-producing F. asiaticum strains were isolated and compared regarding their ability to produce NIV. Strain RR108 had the highest yield of NIV among 62 field isolates surveyed and was then genetically modified for higher production. Targeted deletion of the FaFlbA gene, encoding a regulator of G protein signalling protein, resulted in a significant increase in NIV and 4-AcNIV production in the FaFlbA deletion mutant ΔFaFlbA. The expression of three TRI genes involved in the trichothecene biosynthetic pathway was upregulated in ΔFaFlbA. ΔFaFlbA produced the highest amount of NIV and 4-AcNIV when cultured in brown long-grain rice for 21 days, and the yields were 2.07 and 2.84 g kg −1 , respectively. The mutant showed reduced fitness, including reduced conidiation, loss of perithecial development and decreased virulence on wheat heads, which makes it biologically safe for large-scale preparation and purification of NIV and 4-AcNIV.Conclusions: The F. asiaticum mutant strain ΔFaFlbA presented improved production of NIV and 4-AcNIV with reduced fitness and virulence in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The FaFlbA mutant of Fusarium asiaticum is significantly increased in nivalenol production

Fang

Dong

Wang

et al. 2022

Journal of Applied Microbiology

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“…For instance, LLC was used to explore the presence of pesticides (namely methomyl, fenobucarb, and carbaryl pesticides) in vegetable and fruit samples (Ito et al, 2008). LLC was also successfully applied in the isolation of mycotoxins from foods, for example, Alternaria mycotoxins from wine and juice (Fan et al, 2016), Fusaria B‐type fumonisins (Hu et al, 2020) and trichothecene mycotoxins from Fusarium (Liu et al, 2018). In these studies, LLC has shown to be more advantageous over hitherto used techniques, such as column chromatography on macroporous resins, anion‐exchange resins, or octadecylsilyl silica.…”

Section: Toxinsmentioning

confidence: 99%