Preparative Isolation and Purification of Seven Isoflavones from <i>Belamcanda chinensis</i>

Lee, Yeon Sil; Kim, Seon Ha; Kim, Jin Kyu; Lee, Sang‐Hyun; Jung, Sang Hoon; Lim, Soon Sung

doi:10.1002/pca.1306

Cited by 35 publications

(17 citation statements)

References 26 publications

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“…The most important step in the design of an HSCCC separation system is the selection of the solvent system. Generally, two-phase solvent systems need to satisfy the following requirements: (1) the settling time of the solvent system should ideally be shorter than 30 s to ensure satisfactory retention of the stationary phase; (2) the partition coefficient (K) of the target compounds should be 0.5 ≤ K ≤ 2.0 for efficient separation; (3) the separation factor between the two components (α = K2/K1, K2 > K1) should be greater than 1.5 (Lee et al, 2011); and (4) the sample should not decompose or be denatured. In the current study, the K values of the four stilbenes were determined via HPLC, as described in the experimental section (Table 1).…”

Section: Results and Discussion Hplc Analysis And Selection Of The Twmentioning

confidence: 99%

Separation and Purification of Four Stilbenes from Vitis vinifera L. cv. Cabernet Sauvignon Roots Through High-speed Counter-current Chromatography

Wei¹,

Pi²,

Ma³

et al. 2016

SAJEV

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A method for preparative separation and purification of trans-resveratrol, δ-viniferin, ε-viniferin and trans-vitisin B from the roots of Vitis vinifera L. cv. Cabernet Sauvignon was successfully established and is reported on in this paper. The four important stilbenes were purified by high-speed counter-current chromatography (HSCCC) with a suitable quaternary solvent system composed of chloroform-methanoln-butanol-water (4:3:0.05:2, v/v). A total of 7.1 mg ± 0.2 mg of trans-resveratrol, 1.1 mg ± 0.1 mg of δ-viniferin, 18.7 mg ± 0.5 mg of ε-viniferin, and 12.2 mg ± 0.2 mg of trans-vitisin B, with purities of 97.89%, 90.61%, 94.37% and 78.38% respectively, were obtained from 241 mg of crude sample in a one-step HSCCC separation. The chemical structures of trans-resveratrol and δ-viniferin were further confirmed with the retention time using the method of standard addition, while the structural identification of ε-viniferin and trans-vitisin B was performed with LC-ESI/MS, 1 H-NMR, and 13 C-NMR.

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Section: Results and Discussion Hplc Analysis And Selection Of The Twmentioning

confidence: 99%

Separation and Purification of Four Stilbenes from Vitis vinifera L. cv. Cabernet Sauvignon Roots Through High-speed Counter-current Chromatography

Wei¹,

Pi²,

Ma³

et al. 2016

SAJEV

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“…To date, successful applications of RP-FC and HSCCC have been reported for the purification of a number of active components in natural products (Blunt et al, 1987;Raman et al, 2005;Lee et al, 2011;Mudge et al, 2013). Most of the previous studies reported the separation process using either method, however, to the best of our knowledge, little attention has been paid to the comparison of the two techniques.…”

Section: Introductionmentioning

confidence: 99%

One-step Separation and Purification of Two Chromones and One Pyrone from Aloe barbadensis Miller: a Comparison Between Reversed-phase Flash Chromatography and High-speed Counter Current Chromatography

et al. 2014

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“…HSCCC has great advantages of 100% sample recovery, simplicity to scale up and high yield of sample. Therefore, this technique has been extensively applied for separation and purification of natural products (Ito et al, 2011;Lee et al, 2011;Shi et al, 2011;Hu et al, 2008), especially for flavonoids (Liu et al, 2005;Yang et al, 2009;Wu et al, 2009;Wei et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation of chemical compounds from Sorbus tianschanica Rupr by high-speed counter-current chromatography

Yu¹,

Zheng²,

Le³

et al. 2012

J. Med. Plants Res.

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Sorbus tianschanica Rupr has been used as a pharmacologically valuable plant in Xinjiang to treat tuberculosis, asthma, cough and gastritis for a long time. Four compounds including three flavonoids and chlorogenic acid were isolated and purified by high-speed counter-current chromatography (HSCCC) from S. tianschanica Rupr. The two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (2:6:1:8, v/v). Consequently, chlorogenic acid (1), Quercetin-3-O-(6''-Omalonyl)-β-D-glucoside (2), Kaempferol-3-O-(6''-O-malonyl)-β-D-glucopyranoside (3) and Kaempferol-3-O-β-D-glucopyranoside (4) were obtained from 250 mg crude extracts with purities of 93, 91, 89 and 96%, respectively in a single run. The chemical structures were confirmed by mass spectrometer (MS), 1 H nuclear magnetic resonance (NMR) and 13 C NMR. To our knowledge, compounds (2) and (3) were obtained from S. tianschanica Rupr for the first time.

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Preparative Isolation and Purification of Seven Isoflavones from Belamcanda chinensis

Abstract: Two isoflavone glycosides and five isoflavone derivatives were successfully isolated and purified from the crude methanol extract of dried rhizomes of the B. chinensis by HSCCC.

Cited by 35 publications

References 26 publications

Separation and Purification of Four Stilbenes from Vitis vinifera L. cv. Cabernet Sauvignon Roots Through High-speed Counter-current Chromatography

Separation and Purification of Four Stilbenes from Vitis vinifera L. cv. Cabernet Sauvignon Roots Through High-speed Counter-current Chromatography

One-step Separation and Purification of Two Chromones and One Pyrone from Aloe barbadensis Miller: a Comparison Between Reversed-phase Flash Chromatography and High-speed Counter Current Chromatography

Isolation of chemical compounds from Sorbus tianschanica Rupr by high-speed counter-current chromatography

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