Isolation of chemical compounds from Sorbus tianschanica Rupr by high-speed counter-current chromatography

Abstract

Sorbus tianschanica Rupr has been used as a pharmacologically valuable plant in Xinjiang to treat tuberculosis, asthma, cough and gastritis for a long time. Four compounds including three flavonoids and chlorogenic acid were isolated and purified by high-speed counter-current chromatography (HSCCC) from S. tianschanica Rupr. The two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (2:6:1:8, v/v). Consequently, chlorogenic acid (1), Quercetin-3-O-(6''-Omalonyl)-β-D-glucoside (2), Kaempferol-3-O-(6''-O-malonyl)-β-D-glucopyranoside (3) and Kaempferol-3-O-β-D-glucopyranoside (4) were obtained from 250 mg crude extracts with purities of 93, 91, 89 and 96%, respectively in a single run. The chemical structures were confirmed by mass spectrometer (MS), 1 H nuclear magnetic resonance (NMR) and 13 C NMR. To our knowledge, compounds (2) and (3) were obtained from S. tianschanica Rupr for the first time.

Phytoprofiling of Sorbus L. Inflorescences: A Valuable and Promising Resource for Phenolics

“…Furthermore, Sorbus' extraordinary apomixis, cross-breeding, and intergeneric hybridization abilities have led to a great expansion of Sorbus genotypes, cultivars, hybrids, and varieties [8,9]. Almost all parts of the Sorbus plants have a historical record of usage in various traditional medicinal systems, as well as in food, fodder, beauty, health, and wellness-promoting products [10][11][12][13][14]. The fruits have been included in pharmacopoeias as polyvitaminic substances and in daily nutrition, but, due to their tart and specific taste, they are neglected or only used occasionally, although the ascorbic acid content exceeds the amount in oranges [4,15,16].…”