Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide

Seely, G. R.; Duncan, Mary Jo; Vidaver, William

doi:10.1007/bf00350754

Cited by 211 publications

(117 citation statements)

References 13 publications

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“…The stipe tissues used weighed about 0.5 g and the holdfast tissue 0.3 g. Pigments were always subjected to analyses within l h of harvesting. Chlorophyll a , chlorophyll c and fucoxanthin contents were obtained following the DMSOextraction procedure, described by Seely and coworkers (Seely et al 1972) and modified by Wheeler (1980). Tissues were generally extracted until they were bleached.…”

Section: Methodsmentioning

confidence: 99%

Aspects of carbon metabolism in relation to autumnal blade abscission in the kelp Pleurophycus gardneri (Phaeophyceae, Laminariales

Germann¹

1989

Mar. Ecol. Prog. Ser.

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Pre-abscission changes in the deciduous kelp Pleurophycus gardneri involve a gradual decrease in dry weight, mannitol and laminaran content. These carbon compounds decreased from about 18% (dry weight) to 4 % prior to blade abscission, while the perennial structures, stipe and holdfast, showed a concomitant increase in mannitol and dry weight towards autumn. In November, at the time of new blade outgrowth, mannitol content peaked in stipe and holdfast with 12 and 1 8 % , respectively. Laminaran accounted for less than 2 % in all thallus parts and showed no measurable seasonal variation. Pigment levels of the blade increased with progressing senescence and were at maximum just prior to blade abscission. The photosynthetic performance of the blade revealed saturating irradiances of about 50pE m-2 S-' and a compensation irradiance of 2.5 to 3~t E m-2 S-' measured in April. h g h t saturation of the stipe was at 38 pE m-2 S-' and of the haptera at 23 pE m-2 S-' while compensation irradiances were at roughly 8 FE m-2 S-' for both perennial parts. It is suggested that new blade outgrowth requires 11ght-carbon fixation. No acropetal translocation of the abundant carbon store of the perennial structures was observed The deciduous habit of P. gardneri reflects a shade-adapted growth strategy. The perennial parts are involved In the overall physiology of the plant and have similar seasonal fluctuations in their constituents to those of the blade. The described growth strategy is novel among perennial kelp species.

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Section: Methodsmentioning

confidence: 99%

Aspects of carbon metabolism in relation to autumnal blade abscission in the kelp Pleurophycus gardneri (Phaeophyceae, Laminariales

Germann¹

1989

Mar. Ecol. Prog. Ser.

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Section: Methodsmentioning

confidence: 99%

“…Chlorophyll (a + c) and fucoxanthin were extracted from fresh tissue samples using a 2-step DMSO (dimethylsulfoxide), methanol-acetone extraction procedure. Pigments were measured spectrophotometrically, and pigment concentrations were calculated following Seely et al (1972).Statistical analysis. Monthly gross P versus E curves were constructed for each tissue type using the exponential function of Webb et al (1974).…”

mentioning

confidence: 99%

Dynamics of carbon allocation in a deep-water population of the deciduous kelp Pleurophycus gardneri (Laminariales)

Dominik¹,

Zimmerman²

2006

Mar. Ecol. Prog. Ser.

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Pleurophycus gardneri (Laminariales) is common in the low intertidal of the Northeast Pacific, but dominates many deep (30 to 40 m) rocky reefs in central California. Seasonal dynamics of productivity and resource allocation of a deep-water population of this deciduous, stipitate kelp were studied to understand how blade abscission affects the annual carbon budget. Patterns of growth, metabolism, and carbon storage and mobilization were measured monthly for 1 yr relative to in situ light and temperature, and used to model the annual carbon budget. The resulting carbon budget was used to determine if blade abscission effectively reduced respiratory demand during the winter period of low light availability. Metabolic properties (photosynthesis, photoacclimation, and respiration) were seasonally constant and showed evidence of photoacclimation to this deep, low-light environment. Blades grew between February and July, followed by senescence and sloughing from August to December. Concentrations of laminaran and mannitol increased in the blades from the onset of sloughing in August until just prior to blade abscission in mid-December, suggesting translocation of these carbohydrates may have occurred from the blade to the stipe and holdfast. Carbon budget estimates revealed that scalar irradiance measures overestimated the light available for photosynthesis of these paddle-shaped kelp blades by 50 to 75%. The calculations also revealed that blade retention allowed for the maintenance of positive carbon balance throughout the year. Thus, conservation of the internal carbon reserve for metabolic survival during the low-light period does not appear to be a viable explanation for the deciduous life history of P. gardneri. Abscission may reduce hydrodynamic drag, thus minimizing the probability of dislodgment of entire plants during winter storm events, or promote spore dispersal as abscised blades and sori drift away from the parent holdfast. KEY WORDS: Carbon budget · Photosynthesis · Abscission · Light acclimation Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 309: [143][144][145][146][147][148][149][150][151][152][153][154][155][156][157] 2006 excluded by light limitation (Edwards 1998). However, there is no clear evidence that the newly settled algae undergo a seasonally adjusted metabolic response to light availability. In northern latitudes where light limitation is seasonally predictable, perennial kelps have evolved a variety of physiological mechanisms that allow them to survive through summer periods of nutrient limitation and winter periods of light limitation (Gagne et al. 1982). Laminaria solidungula, for example, uses stored carbohydrates accumulated during summer to complete 90% of its annual linear blade growth during the virtual darkness of the Arctic winter (Mann 1973, Chapman & Craigie 1977, 1978, Dunton & Schell 1986. Another mechanism may involve cellular photoacclimation to increased photosynthetic efficiency in low-light environments, thr...

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“…Total chlorophyll and total carotenoids were measured using spectrophotometer (Jenway, 6405 UV/vis) at (468 and 666 nm, respectively) according to Seely et al, (1972). The total chlorophyll and total carotenoids concentrations were calculated with the following equations:…”

Section: Pigments Contentsmentioning

confidence: 99%

Human Anticancers and Antidiabetic Activities of the Cyanobacterium Fischerella sp. BS1-EG Isolated from River Nile, Egypt

Ahmed¹,

Badawi²,

Mostafa³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide

Cited by 211 publications

References 13 publications

Aspects of carbon metabolism in relation to autumnal blade abscission in the kelp Pleurophycus gardneri (Phaeophyceae, Laminariales

Aspects of carbon metabolism in relation to autumnal blade abscission in the kelp Pleurophycus gardneri (Phaeophyceae, Laminariales

Dynamics of carbon allocation in a deep-water population of the deciduous kelp Pleurophycus gardneri (Laminariales)

Human Anticancers and Antidiabetic Activities of the Cyanobacterium Fischerella sp. BS1-EG Isolated from River Nile, Egypt

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