Preparative Analog to Thin Layer Chromatography.

Abstract

acid dehydrogenase, LAD, in concentrations as low as 0.000470 unit per ml. (Table I).This reaction was also first order with respect to diaphorase in concentrations of 0.0004 to 0.080 unit per ml. of solution, and to resazurin (10^to 10_6ilf). Hence the concentrations of these materials may be easily determined by this technique. Also, the rate of formation of resorufin was proportional to the NAD+ concentrations in the region 10~w to 10-7M, thus allowing an easy, direct determination of the quantity of this eoenzyme present. By the use of ethyl alcohol as substrate, the quantity of alcohol dehydrogenase as low as 0.000303 unit per ml. may be determined.Full details on the systems described herein, as well as application to other systems, will be given in an article to follow.

Preparative Layer Chromatography

“…the solvent covers a distance of 16-5 cm., but if repeated five times the travelling distance corresponds to almost 65 cm., t h a t is a fourfold increase of the number of separating steps. Assuming t h a t after t h e first development 16 compounds are uniformly distributed on the plate, three subsequent runs will transport the upper 5 compounds (16 through 12) to the solvent front, two further runs the following three (11 through 9). 1.…”