DC von Steroiden und verwandten Verbindungen

Neher, R.

doi:10.1007/978-3-642-49187-0_13

Cited by 2 publications

(3 citation statements)

References 160 publications

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“…The Rf‐values were calculated individually for all spots on each TLC‐plate according to the standards 11 . The corresponding spots were excised from the TLC‐plate and the radioactivity eluted with 2 × 5 mL of acetone.…”

Section: Methodsmentioning

confidence: 99%

“…After autoradiography the left and right lanes of the TLC-plates were cut as 3 cm stripes and sprayed (in a fume hood) with an equal mixture of concentrated sulphuric acid and methanol. 11 The stripes were heated on an oven until the spots of the steroid standards appeared in their individual colour. For documentation these stripes were sealed with a plastic sheet (to avoid oxidation) and stored at room temperature.…”

Section: Metabolism Of Dehydroepiandrosterone and Testosteronementioning

confidence: 99%

“…The Rf-values were calculated individually for all spots on each TLC-plate according to the standards. 11 The corresponding spots were excised from the TLC-plate and the radioactivity eluted with 2 × 5 mL of acetone. After evaporation of the acetone, 6 mL scintillation fluid was added, the counts per min (cpm) were recorded and the decays per min (dpm) calculated.…”

Section: Metabolism Of Dehydroepiandrosterone and Testosteronementioning

confidence: 99%

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In vitrometabolism of dehydroepiandrosterone and testosterone by canine hair follicle cells

Bamberg

Aichinger

Mitteregger

2004

Veterinary Dermatology

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The metabolism of radioactive dehydroepiandrosterone (DHEA) and testosterone was studied in dermal papilla cells (DPC) and dermal fibroblasts (DFB) derived from hair follicles from two different body sites (head, flank) of four male, castrated beagle dogs. Thin layer chromatography was used for separation, and autoradiography for identification of the radioactive metabolites. DHEA was metabolized mainly to 11 alpha-OH-testosterone and only to a minor extent to 11 alpha-OH-androstenedione and another unidentified metabolite. The highest percentage of metabolization of DHEA was found in DFB of the head. Testosterone was metabolized only to a minor extent (less than 10%) to 5 alpha-dihydrotestosterone and epiandrosterone and there was no significant difference between either the two cell types or the two locations. These results clearly show that the metabolization of androgens in canine DPC and DFB is different from that observed in cells from the human hair follicle.

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Section: Methodsmentioning

confidence: 99%

Section: Metabolism Of Dehydroepiandrosterone and Testosteronementioning

confidence: 99%

Section: Metabolism Of Dehydroepiandrosterone and Testosteronementioning

confidence: 99%

See 1 more Smart Citation

In vitrometabolism of dehydroepiandrosterone and testosterone by canine hair follicle cells

Bamberg

Aichinger

Mitteregger

2004

Veterinary Dermatology

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Biotransformation of 5?H-pregnan-3?ol-20-one and cardenolides in cell suspension cultures of Nerium oleander L.

Paper

Franz

1990

Plant Cell Reports

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In order to demonstrate enzyme activities playing a role in the biosynthesis of cardenolides and 2,6-dideoxysugars, 5βH-pregnan-3βol-20-one and cardenolides (digitoxigenin, oleandrigenin/L-oleandrose, oleandrin, neriifolin, digitoxigeninmonodigitoxoside and strospeside) were fed to cell suspension cultures of Nerium oleander L.. It could be shown that cell suspension cultures of Nerium oleander L. are able to oxidize, isomerize and glucosylate 5βH-steroidaglycones at C-3. The respective glucosides of the 5βH-steroid-aglycones are the main biotransformation products. These cell cultures are an appropriate tool for the production of labelled 5βH-steroidglucosides.

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DC von Steroiden und verwandten Verbindungen

Cited by 2 publications

References 160 publications

In vitrometabolism of dehydroepiandrosterone and testosterone by canine hair follicle cells

In vitrometabolism of dehydroepiandrosterone and testosterone by canine hair follicle cells

Biotransformation of 5?H-pregnan-3?ol-20-one and cardenolides in cell suspension cultures of Nerium oleander L.

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