Preparation, structural characterization, and bioactivities of polysaccharides from Psidium guajava: A review

Recent trends in extraction, purification, structural characterization, and biological activities evaluation of Perilla frutescens (L.) Britton polysaccharide

“…For example, Li et al (34) eluted four types of P. frutescens leaf polysaccharides on a DAE-Cellulose 52 column (2.6 × 30 cm) at a flow rate of 0.6 mL/min using different concentrations of sodium chloride solution (0, 0.1, 0.2, 0.3, and 0.5 mol/L). Gel-column chromatography (Sephadex-G series and Sephacryl-S series) is an effective method to separate polysaccharides with different molecular weights (47). High-molecular-weight polysaccharides will be eluted first with the mobile phase, while the low-molecular-weight polysaccharides will diffuse into the pores of the gel and elute later (48).…”

“…The acid hydrolysis method is used to completely break the glycosidic bonds of polysaccharides; after hydrolysis, the polysaccharide sample is neutralized, filtered, and derivatized for analysis of monosaccharide composition (57). High-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), gas chromatography (GC), gas-liquid chromatography, and ion chromatography (IC) are common methods employed to measure the monosaccharide composition and molar ratio of polysaccharides (15,16,47,48). Using GC-MS analysis, the monosaccharide composition of a PSMP was determined to comprise rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), and galactose (Gal) at a mass ratio of 3.196%: 43.901%: 21.956%: 4.244%: 4.706%: 21.997% (18).…”

Application of ionic liquid ultrasound-assisted extraction (IL-UAE) of lycopene from guava (Psidium guajava L.) by response surface methodology and artificial neural network-genetic algorithm

“…Guava ( Psidium guajava L.) is an important crop belonging to the genus Psidium and the Myrtaceae family, originating in the tropics of South America. It was brought to many parts of the world and is now naturalized there [1] . Guava is abundant in V C , soluble sugar, soluble protein, and various polyphenolic substances [2] .…”

Optimization of Extraction Process, Preliminary Characterization and Safety Study of Crude Polysaccharides from Morindae Officinalis Radix

“…The evidence suggests that the biological activities are related to structural characteristics of polysaccharides (chemical structure, monosaccharide composition, molecular weight, etc.) affected by the selected methods of extraction [ 40 , 41 , 42 ]. The extraction methods of polysaccharides are becoming increasingly diverse, including hot water extraction, alkali solution extraction, ultrasound-assisted extraction, microwave-assisted extraction, subcritical water extraction, and so on.…”