Preparation of β-CD-DPPE-Dox Nanomedicine and Its’ Application as the Anticancer and Antitumor Drug

Abstract

β-CD-DPPE molecule was synthesized through the conjugation of β-CD-NH2 and the DPPE molecule, and its’ water-solubility was more excellent than the traditional phospholipid molecule. The spherical micelles was formed by β-CD-DPPE molecule in aqueous solution, and the β-CD-DPPE-Dox nanomedicine can be prepared through loading Dox (Doxorubicin) into the micelles, and the Dox loading ratio was about 82.3 ± 7.27%. At the same time the Dox release behavior from the nanomedicine was sustained-release and pH controlled release, and the release test in vitro showed that the release rate of the Dox at the lower pH was faster than that of normal pH (pH = 7.4), which indicated that the rate of release in the tumor microenvironment is faster than in the normal tissue. Biological test showed that the micelles was low cytotoxicity, and the cytotoxicity of β-CD-DPPE-Dox nanomedicine was lower than the Dox under the same Dox concentration, and the β-CD-DPPE-Dox nanomedicine could effectively induce cancer cell apoptosis and inhibit the tumor growth.

Construction of a Drug Delivery System and Photodynamic Therapy Reagent Based on the Biotin-HSA-DDA-TCPP Molecules and the Application of Synergistic Antitumor Effect

“…Seen from Figure 4a, the relative viability of HepG2 cells and HepG2 cells was about 90% for 24 h incubation when the biotin-HSA-DDA-TCPP aggregates reached 30 μg•mL −1 , and the relative cell viability was 81.4% for 48 h incubation, which indicated that the biotin-HSA-DDA-TCPP aggregates have low toxicity, and the reason may be due to the excellent biocompatibility of aggregates, which are endowed by HSA; nontoxicity of aggregates is a prerequisite for the use as a drug carrier. 30 Figure 4a,c shows that the biotin-HSA-DDA-TCPP-Dox nanoparticles (30 μg• mL −1 ) were incubated with HepG2 cells and H22 cells for 24 h, and the cell relative viabilities were 70 and 65%, respectively. Figure 4b,d shows that the relative cell viabilities were 39 and indicated that the nanoparticles had a stronger effect on HepG2 cells than on H22 cells.…”

Delay Tumor Growth Properties of LA-PEG-G Molecule and Its' Application as Drug Carrier

“…The amount of Dox released was measured at 37 °C. Buffer solution (4 mL) was taken out from the buffer solution outside the dialysis bag for UV-visible spectrophotometer detection (detection wavelength is 485 nm) at different times (1,2,4,8,12,24,36,48,72,84,96,108, and 120 h), whereas 4 mL fresh buffer solution was added into the buffer solution outside the dialysis bag.…”

“…The Zeta potential of the LA-PEG-G aggregates was −35.80 mV in the aqueous solution, which indicated that the surface of the LA-PEG-G aggregates was negatively charged in aqueous solution. A high Zeta potential allows the LA-PEG-G aggregates to maintain good dispersion in aqueous solution [24,25] . The PEG in the LA-PEG-G molecules can help the LA-PEG-G aggregates escape from the trap of Kupffer cells and macrophages in the spleen through scavenger receptors without activating MPS, which help the blood circulation of the LA-PEG-G aggregates in the body [25] .…”