Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties

Gong, Bolin; Ke, Cong‐Yu; Geng, Xindu

doi:10.1007/s00216-003-1805-5

Cited by 29 publications

(20 citation statements)

References 16 publications

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“…These beads have been used as chro-matographic packing in size exclusion chromatography (SEC) and have shown a good performance. This method has also been adopted for the preparation of chromatographic packings for hydrophobic interaction chromatography (HIC) and ion-exchange chromatography (IEC) [17,18].…”

Section: Introductionmentioning

confidence: 99%

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

et al. 2006

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A single-stage swelling and polymerization method was proposed for the synthesis of monodisperse porous poly(vinyl acetate-co-divinylbenzene) [poly(VAc-co-DVB)] particles with different VAc/DVB feed ratios. The particles obtained with the VAc/DVB feed ratio of 50:50 v/v had a narrow pore size distribution exhibiting a sharp peak at 30 nm. Based on this distribution the mean pore size and the specific volume were determined as 12 nm and 1.39 mL/g, respectively. The specific surface area of poly(VAc-co-DVB) particles was found to be 470 m2/g. These properties make poly(VAc-co-DVB) particles a promising support for potential HPLC applications. Poly(vinyl alcohol-co-divinylbenzene) [poly(VA-co-DVB)] particles were then obtained by the basic hydrolysis of poly(VAc-co-DVB) particles. The hydroxyl groups on poly(VA-co-DVB) particles have a suitably reactive functionality for surface grafting or derivatization protocols aiming at synthesizing various HPLC packings. The examination of poly(VA-co-DVB) particles by confocal laser scanning microscopy showed the homogeneous distribution of hydroxyl functionality in the particle interior. As a starting point, the chromatographic performance of plain material, poly(VAc-co-DVB) particles produced with VAc/DVB feed ratio of 50:50 (v/v) was tested by a commonly utilized chromatographic mode, reversed phase chromatography. Poly(VAc-co-DVB) particles were successfully used as packing material in the RP separation of alkylbenzenes with resolutions higher than 1.5. Theoretical plate numbers up to 17 500 plates/m were achieved. No significant change both in the chromatographic resolution and column efficiency was observed with increasing flow rate. The chromatography showed that poly(VAc-co-DVB) particles were a suitable starting material for the synthesis of chromatographic packings for different modes of HPLC.

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Section: Introductionmentioning

confidence: 99%

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

et al. 2006

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“…In our laboratory, we have prepared different MIPs films by the approach using methacrylic acid, acrylamide or 2-VPY as functional monomers, EDMA or trimethylolpropane trimethacrylate as crosslinkers. Because the morphology of the resulting beads is mainly determined by the P GMA-EDMA beads, and the P GMA-EDMA beads' pore sizes and particles sizes can be easily controlled by changing synthesis conditions, 26,29 which gives access to monodisperse, spherical imprinted particles with different pore sizes and particles sizes. Moreover, MIPs films prepared by surface grafting using P GMA-EDMA beads as supports can be used in a wide pH range from 1 to 14.…”

Section: Discussionmentioning

confidence: 99%

Preparation of molecularly imprinted polymer films on monodisperse macromolecular beads

Zheng

Wei-ping

Fan

2009

J of Applied Polymer Sci

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A new and facile technique for preparing homogeneous and highly selective molecularly imprinted polymers (MIPs) films on porous monodisperse poly-(glycidyl methacrylate-co-ethylene glycol dimethacrylate) beads (P GMA-EDMA beads) has been described: before polymerization, initiator was immobilized on the P GMA-EDMA beads' surface by chemical reactivity; then functional monomers and crosslinkers were initiated by the surface-bound initiator and copolymerized to form MIPs films on the P GMA-EDMA beads' surface in the presence of template molecules. The resulting beads were analyzed by FTIR spectroscopy and X-ray photoelectron spectra. The effects of the initiator amount and copolymerization time on the content of MIPs, and MIPs contents on morphologies and pore properties of the resulting beads were investigated. The results show that the content of MIPs can be adjusted by changing initiator amount or by controlling copolymerization time. The binding experiments indicate that, at lower grafting level, with the increase of MIPs content, the adsorption and recognition capabilities of the resulting beads enhance. When MIPs content increase to 16.75%, the highest adsorption and recognition capabilities are obtained.

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“…In the ion exchange chromatography, varying the charge density of the ion-exchanger, the retention time of solutes on the stationary phase was changed. 21,22 In this paper, the ion-exchanger in the polymeric form on a long chain served a large number of charge density, and more effective interaction with the solutes with respect to the ionic groups attached onto the support, so the retention times were increased.…”

Section: Chromatographic Separation Of Proteins On the Synthesized Momentioning

confidence: 99%

Preparation of High-capacity, Monodisperse Polymeric Weak Cation Exchange Packings Using Surface-initiated Atom Transfer Radical Polymerization and Its Chromatographic Properties

CHANG¹,

Wang²,

Gong³

2011

Chin. J. Chem.

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A novel stationary phase for weak cation exchange (WCX) chromatography was prepared by "grafting from" strategy. Surface initiated atom transfer radical polymerization (ATRP) of acrylic acid (AA) was conducted in toluene medium, starting from the macromolecule initiators of poly(4-vinylbenzyl chloride-co-divinylbenzene) (P CMS/DVB ) beads. The amounts of poly(acrylic acid) grafted chains with different ATRP formulations were calculated based on the elemental analyses. The poly(acrylic acid) grafted beads obtained with different ATRP formulations were tried as chromatographic packings in the separation of proteins by ion-exchange chromatography. The effect of the poly(acrylic acid) grafted chain lengths on P CMS/DVB beads for the separation of proteins was investigated in details. Simultaneously, characterization of the column was investigated as ion chromatographic stationary phase for the separation of inorganic cations. The results show that poly(acrylic acid) grafted columns had excellent performance for separation of proteins and inorganic cations. The highest of the dynamic capacity of the column was 35.55 mg/mL. The columns were provided with high column efficiency.Keywords atom transfer radical polymerization, monodisperse poly(4-vinylbenzyl chloride-co-divinylbenzene)-(P CMS/DVB ) beads, weak cation exchange chromatographic stationary phase, proteins, inorganic cations www.cjc.wiley-vch.de

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Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatographic properties

Cited by 29 publications

References 16 publications

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

Preparation of molecularly imprinted polymer films on monodisperse macromolecular beads

Preparation of High-capacity, Monodisperse Polymeric Weak Cation Exchange Packings Using Surface-initiated Atom Transfer Radical Polymerization and Its Chromatographic Properties

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