Preparation of the sodium salt of high acyl gellan and characterization of its structure, thermal and rheological behaviors

Murillo-Martínez, María M.; Tecante, Alberto

doi:10.1016/j.carbpol.2014.02.056

Cited by 12 publications

(11 citation statements)

References 35 publications

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“…In its native state, these tetramers also contain glycerate and acetate functionalities, however the hot alkaline conditions employed in GG extraction leads to their reduction to hydroxyl residues ( Figure 2). Works exploring the functional impacts of deacylation may be found in [30][31][32][33] , however TE with gellan gum is most commonly performed using the deacylated form due to the relative ease of isolation and processing. In hot aqueous dispersion, both high and low-acyl gellan gum chains are randomly distributed in solution, but self-assemble into helix pairs upon cooling through a sol-gel transition temperature (~50 ˚C).…”

Section: Structurementioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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Section: Structurementioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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“…44 The position of the peaks varied during the melting and the crystallization events and may be explained by the thermal hysteresis. 45 The enthalpy ( H m and H c ) and entropy ( S m and S c ) of the melting and the crystallization events were calculated from the amount of heat absorbed and released during the events, respectively. Both enthalpy and entropy during the events were higher in the formulations containing higher concentrations of SMS and water.…”

Section: Thermal Analysismentioning

confidence: 99%

Ultrasonication-Assisted Preparation and Characterization of Emulsions and Emulsion Gels for Topical Drug Delivery

Singh

Behera

Pramanik

et al. 2015

Journal of Pharmaceutical Sciences

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“…HAG shows considerably higher transition temperatures than LAG for the same concentrations of polymer and external counterions (Mazen et al ., ). Some T m and T g values for HAG are available in the literature (Morris et al ., ; Mazen et al ., ; Huang et al ., ; Flores‐Huicochea et al ., ; Murillo‐Martínez & Tecante, ). In general, lack of hysteresis and increase in T m and T g with cation concentration are observed.…”

Section: Introductionmentioning

confidence: 99%

“…In general, lack of hysteresis and increase in T m and T g with cation concentration are observed. Regarding the purity of HAG, reports include a commercial form without further treatment (Huang et al ., ; Flores‐Huicochea et al ., ), modified or purified preparations (Morris et al ., ; Goh et al ., ; Murillo‐Martínez & Tecante, ) and laboratory‐produced and purified samples (Mazen et al ., ; Goh et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, chemical synthesis has been used to modify the structure of LAG (Coutinho et al ., ; Novac et al ., ; Karthika & Vishalakshi, ), but similar modifications on HAG have not been reported. In a previous investigation (Murillo‐Martínez & Tecante, ), a method to obtain a highly purified NaHAG and its characterisation and transition temperatures without addition of external counterion was reported. However, more studies about the functional properties of modified gellan, especially HAG, and its physicochemical characteristics are needed to understand the properties of particular forms and how they differ from those of commercial preparations which normally contain high concentrations of mono‐ and divalent cations.…”

Section: Introductionmentioning

confidence: 99%

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Thermal stability and transition temperatures of monovalent salts of high‐acyl gellan gels

Murillo-Martínez

Tecante

2015

Int J of Food Sci Tech

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Thermogravimetry/derivative thermogravimetry (TG/DTG), rheometry and differential scanning calorimetry (DSC) were used to study the thermal stability and determine the transition temperatures of the sodium and potassium salts of high-acyl gellan (HAG) in the presence of 0-100 mM NaCl and KCl, respectively. TG/DTG revealed the potassium gellan (KHAG) gels to be more stable than those of sodium gellan (NaHAG), regardless of external cation concentration. Rheometry and DSC showed the melting (T m ) and gelling (T g ) temperatures to increase with cation concentration. The DSC peak temperatures showed thermal hysteresis contrary to rheometry. In most cases, DSC revealed KHAG to exhibit higher T m and T g than NaHAG. Consequently, thermal characteristics of NaHAG and KHAG gels depend on the size of the external cation and its ability to coordinate water molecules. Cation salts of HAG exhibit significantly lower transition temperatures than the commercial preparation from which they were produced.

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Preparation of the sodium salt of high acyl gellan and characterization of its structure, thermal and rheological behaviors

Cited by 12 publications

References 35 publications

Tissue engineering with gellan gum

Tissue engineering with gellan gum

Ultrasonication-Assisted Preparation and Characterization of Emulsions and Emulsion Gels for Topical Drug Delivery

Thermal stability and transition temperatures of monovalent salts of high‐acyl gellan gels

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