Preparation of Symmetrical and Unsymmetrical DNA-Protein Conjugates with DNA as a Molecular Scaffold

Tomkins, Justin M.; Nabbs, Brent K.; Barnes, Karen J.; Legido, Marta; Blacker, A. John; McKendry, Rachel A.; Abell, Chris

doi:10.1002/1439-7633(20010504)2:5<375::aid-cbic375>3.0.co;2-i

Cited by 10 publications

(4 citation statements)

References 15 publications

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“…[29] These complexes can also be used to bind other biotinilated products to the already established DNA-streptavidin complex, a virtue of the tetravalency of streptavidin (that could even be expanded). A few remarkable examples of this power are already available: Abell and co-workers prepared asymmetric protein dumbbells through the use of custom prepared modified oligonucleotides that could bind proteins specifically and also work as PCR primers [31] (Figure 1 d). [30] To date, nanoscientists have mainly employed commercially available modified oligonucleotides that were brought onto the market for totally different purposes (mainly for molecular biology).…”

Section: Methodsmentioning

confidence: 99%

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DNA Codes for Nanoscience

Samorı̀

Zuccheri

2005

Angew Chem Int Ed

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The nanometer scale is a special place where all sciences meet and develop a particularly strong interdisciplinarity. While biology is a source of inspiration for nanoscientists, chemistry has a central role in turning inspirations and methods from biological systems to nanotechnological use. DNA is the biological molecule by which nanoscience and nanotechnology is mostly fascinated. Nature uses DNA not only as a repository of the genetic information, but also as a controller of the expression of the genes it contains. Thus, there are codes embedded in the DNA sequence that serve to control recognition processes on the atomic scale, such as the base pairing, and others that control processes taking place on the nanoscale. From the chemical point of view, DNA is the supramolecular building block with the highest informational content. Nanoscience has therefore the opportunity of using DNA molecules to increase the level of complexity and efficiency in self-assembling and self-directing processes.

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Section: Methodsmentioning

confidence: 99%

“…The assemble signal can be, similarly to (b), an oligonucleotide with each half complementary to the particle-bound or the surface-bound oligonucleotides. [31] e) Self-replication of connectivity among oligonucleotides. [31] e) Self-replication of connectivity among oligonucleotides.…”

Section: Exploiting the Code: Dna-mediated Molecular Constructionmentioning

confidence: 99%

DNA Codes for Nanoscience

Samorı̀

Zuccheri

2005

Angew Chem Int Ed

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“…[14] There are currently several methodologies used to link proteins to DNA. Proteins have been assembled onto DNA scaffolds through intervening adapter molecules such as streptavidin [12,[15][16][17][18][19][20] or aptamers. [21,22] Alternately, direct covalent conjugation can be achieved by modification of cysteine or lysine residues [23][24][25][26] or intein modification.…”

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confidence: 99%

A Universal Method for the Preparation of Covalent Protein–DNA Conjugates for Use in Creating Protein Nanostructures

et al. 2007

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DNA has numerous attractive features as a scaffold for nanostructure assembly. Its rigidity, predictable structure, and assembly through complementary hybridization allow DNA to form nanoscale architectures such as cubes, [1] tetrahedra, [2] octahedra, [3,4] and 2D arrays. [5][6][7][8] By introducing proteins into DNA nanostructures, the recognition elements and functionalities that are inherent in proteins can be organized into nanostructured motifs. DNA-scaffolded protein assemblies have been used in immuno-PCR detection methods (PCR = polymerase chain reaction) [9][10][11] to arrange biocatalysts in a series for sequential reactions [12,13] and to organize other nanomaterials. [14] There are currently several methodologies used to link proteins to DNA. Proteins have been assembled onto DNA scaffolds through intervening adapter molecules such as streptavidin [12,[15][16][17][18][19][20] or aptamers. [21,22] Alternately, direct covalent conjugation can be achieved by modification of cysteine or lysine residues [23][24][25][26] or intein modification. [11,27,28] Niemyer and co-workers have employed these protein-DNA conjugates to form fluorescence resonant energy transfer (FRET) systems for use in nanobiotechnology. [29,30] Herein, we demonstrate a fusion-based strategy to regioselectively and covalently label proteins at the C terminus with single-stranded DNA. These protein-oligonucleotide chimeras were then spontaneously assembled into nanoarchitectures by complementary hybridization of the DNA. The covalent attachment strategy described herein yields a short and compact linkage between the protein and DNA molecule that allows for precise control over protein spacing and orientation in the final nanostructure.To achieve selective protein labeling, we use the enzyme protein farnesyltransferase (PFTase) to label a substrate protein containing a C-terminal tetrapeptide tag with an azide-modified isoprenoid diphosphate (1, Scheme 1).

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“…This potential has resulted in the use of DNA scaffolds for the selective arrangement of nanoparticles 12 and proteins. 13 In an effort to explore the use of DNA scaffolds for the organization of dendrimer-based materials through self-assembly, we describe herein a new synthetic strategy for the preparation of DNAdendron conjugates that retain their hybridization capabilities, and the subsequent construction of multicomponent dendrimers.…”

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confidence: 99%