Preparation of <sup>3</sup>H‐labeled ochratoxins

Chang, F.C.; Chu, Fun Sun

doi:10.1002/jlcr.2580120210

Cited by 8 publications

(1 citation statement)

References 10 publications

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“…Tritiated OA (:1Hlabeled OA) was prepared by New England Nuclear Corp. (Boston, Mass.) using the method of Chang and Chu (2). Water-soluble carbodiimide 1-ethyl-3,3-dimethylamino-propyl-carbodiimide (EDPC) was obtained from Story Chemical Corp., Otta Chemical Division (Muskegon, Mich.).…”

Section: Methodsmentioning

confidence: 99%

Production of antibody against ochratoxin A

Chu¹,

Chang²,

Hinsdill³

1976

Appl Environ Microbiol

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Antibody against ochratoxin A was obtained after repeated injection of different protein-ochratoxin A conjugates to rabbits. Among many protein-ochratoxin conjugates tested, bovine serum albumin-ochratoxin A was found to be the best antigen. The antibody is specific for ochratoxins A, C, and T, but is not specific for ochratoxins B, a, and other coumarin derivatives. The sensitivity for ochratoxin A detection using a binding assay is in the range of 0.5 to 10 ng/0.5ml sample. Detailed methods for the preparation of protein-ochratoxin conjugates, preparation of immune serum, and methods for antibody titers are described.

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Section: Methodsmentioning

confidence: 99%

Production of antibody against ochratoxin A

Chu¹,

Chang²,

Hinsdill³

1976

Appl Environ Microbiol

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Ochratoxin A labelled with ¹⁴C or ³H in the phenylalanine moiety

Hult

1986

Labelled Comp Radiopharmac

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SUMMARYOchratoxin A is a mycotoxin produced by Asperqillus and Penici1l.w moulds. The toxin is an amide formed between phenylalanine and the isocoumarin acid ochratoxin a .Ochratoxin A with high specific radioactivity can be obtained, by sub titution of the natural phenylalanine for I4C or %-labelled phenylalanine.Labelled ochratoxin A was formed by condensation of labelled phenylalanine ethylester with an anhydride derivative of ochratoxin a. The intermediate 0-acetyl ochratoxin C was hydrolysed with pig liver esterase and chymotrypsin to afford ochratoxin A. The overall yield of radioactivity was 50%. Specifi activi ies up to 500 Ci/mol and obtained.

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Labelling of ochratoxins with ³H or ¹²⁵I

Schmiedová¹,

Vereš²,

Černý³

et al. 1989

Labelled Comp Radiopharmac

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LABELLING OF OCHRATOXINS WITH 3H OR1 2 5 1 D. Schmiedovd, K . V e r e S , B.Cern9 I n s t i t u t e of Nuclear Biology and Radiochemistry, Czechoslovak Academy of S c i e n c e s , Prague J. Ruprich, I n s t i t u t e of Hygiene and Epidemiology, Prague J. N6meEek , I n s t i t u t e of Microbiology , Czechoslovak Academy of S c i e n c e s , Prague SUMMARY C a t a l y t . i c hydrogenation of o c h r a t o x i n A by c a r r i e r -f r e e 5 t r i t i u m was used t o p r e p a r e H-ochratoxin B w i t h a h i g h s p e c i f i c a c t i v i t y . I o d i n a t i o n of o c h r a t o x i n B by c a r r i e r -f r e e N a ' 251 u s i n g t h e c h l o r a m i n e method y i e l d e d ' 251-ochratoxin w i t h a h i g h s p e c i f i c a c t i v i t y . Another 1 2 5 1 -d e r i v a t i v e of o c h r a t o x i n A was p r e p a r e d by i o d i n a t i o n of an o c h r a t o x i n A -L--t y r o s i n e -m e t h y l e s t e r c o n j u g a t e . A l l t h r e e r a d i o a c t i v e p r ep a r a t i o n s w e r e found t o b e u s e f u l f o r radioimmunoassay. K e y words: Ochratoxin A and B , l a b e l l i n g , t r i t i a t i o n , r a d i o i o d i n a t i o n , t y r o s i n e -m e t h y l e s t e r c o n j u g a t e

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Preparation of ³H‐labeled ochratoxins

Abstract: SUMMARY 3H-Ochratoxins

Cited by 8 publications

References 10 publications

Production of antibody against ochratoxin A

Production of antibody against ochratoxin A

Ochratoxin A labelled with ¹⁴C or ³H in the phenylalanine moiety

Labelling of ochratoxins with ³H or ¹²⁵I

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Preparation of 3H‐labeled ochratoxins

Abstract: SUMMARY 3H-Ochratoxins

Cited by 8 publications

References 10 publications

Production of antibody against ochratoxin A

Production of antibody against ochratoxin A

Ochratoxin A labelled with 14C or 3H in the phenylalanine moiety

Labelling of ochratoxins with 3H or 125I

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Preparation of ³H‐labeled ochratoxins

Ochratoxin A labelled with ¹⁴C or ³H in the phenylalanine moiety

Labelling of ochratoxins with ³H or ¹²⁵I