Production of antibody against ochratoxin A

Abstract

Antibody against ochratoxin A was obtained after repeated injection of different protein-ochratoxin A conjugates to rabbits. Among many protein-ochratoxin conjugates tested, bovine serum albumin-ochratoxin A was found to be the best antigen. The antibody is specific for ochratoxins A, C, and T, but is not specific for ochratoxins B, a, and other coumarin derivatives. The sensitivity for ochratoxin A detection using a binding assay is in the range of 0.5 to 10 ng/0.5ml sample. Detailed methods for the preparation of protein-ochratoxin conjugates, preparation of immune serum, and methods for antibody titers are described.

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

“…Several authors adopt the similar strategy for their conjugation reactions. [20][21][22] Hoechst-modied nanoparticles were obtained and washed three times with deionized water to remove the unreacted dye, separated using a magnetic separation system (Millipore), and stored at 4 C. The pH conditions (3, 5, 7, 9, and 11) were obtained using acetic acid and sodium bicarbonate. The uorescence of Hoechst was measured by an Epoch Spectrophotometer (Biotek, Winooski, VT) using ex 340 nm and em 455 nm.…”

A SIMPLE SOLID‐PHASE RADIOIMMUNOASSAY FOR AFLATOXIN B1

“…With the availability of antibodies having high affinity to aflatoxin B, (afla B , ) and ochratoxin A and the high specific activity of the tritiated mycotoxins, investigations in our and other laboratories have shown that radioimmunoassay (RIA) could be used for the analysis of mycotoxins in foods, feeds and in biological fluids (Chu and Ueno 1977;Chu et al 1976;Langone and Van Vunakis 1976). In the previous studies, both equilibrium dialysis and double-antibody methods have been used for the determination of antibody titers and RIA of afla B1.…”

Directed type IV collagen self‐assembly on hydroxylated PTFE

“…The choice of CNIV was due to its central role in human basal lamina where it serves as a scaffold for the attachment of various, tissue-specific proteins, proteoglycans, and glycosaminoglycans. Coupling of CNIV was achieved by a protocol first reported by Chu et al [21][22][23] A mixture of 30 mg/mL EDC (Sigma) in dry acetone was added to hydroxylated PTFE material in a custom reaction vessel. The coupling reaction proceeded for 1 h at room temperature.…”