Preparation of salvianolic acid A by the degradation reaction of salvianolic acid B in subcritical water integrated with pH-zone-refining counter-current chromatography

Li, Huaizhi; Cheng, Yan; Dong, Hao; Wang, Xiao; Li, Jia; Gao, Qianshan

doi:10.1016/j.chroma.2016.09.039

Cited by 14 publications

(8 citation statements)

References 34 publications

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“…Therefore, we selected SAA for further research. Due to the low content of SAA in S. miltiorrhiza , some researchers transformed salvianolic acid B to SAA by using different reaction conditions, including pH, temperature, pressure, humidity [ 43 ], or subcritical water extraction [ 44 ] to increase the yield of SAA. Oxysophoridine (OSR), an alkaloid extracted from Sophora alopecuroides L, has antioxidant, anti-inflammatory, and antiapoptotic effects on spinal cord injury [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Leng

Kan

et al. 2022

Molecules

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The effective treatment of cervical intraepithelial neoplasia (CIN) can prevent cervical cancer. Salvia miltiorrhiza is a medicinal and health-promoting plant. To identify a potential treatment for CIN, the effect of S. miltiorrhiza extract and its active components on immortalized cervical epithelial cells was studied in vitro. The H8 cell was used as a CIN model. We found that S. miltiorrhiza extract effectively inhibited H8 cells through the CCK8 method. An HPLC–MS analysis revealed that S. miltiorrhiza extract contained salvianolic acid H, salvianolic acid A, salvianolic acid B, monomethyl lithospermate, 9′′′-methyl lithospermate B, and 9′′′-methyl lithospermate B/isomer. Salvianolic acid A had the best inhibitory effect on H8 cells with an IC50 value of 5.74 ± 0.63 μM. We also found that the combination of salvianolic acid A and oxysophoridine had a synergistic inhibitory effect on H8 cells at molar ratios of 4:1, 2:1, 1:1, 1:2, and 1:4, with salvianolic acid A/oxysophoridine = 1:2 having the best synergistic effect. Using Hoechst33342, flow cytometry, and Western blotting analysis, we found that the combination of salvianolic acid A and oxysophoridine can induce programmed apoptosis of H8 cells and block the cell cycle in the G2/M phase, which was correlated with decreased cyclinB1 and CDK1 protein levels. In conclusion, S. miltiorrhiza extract can inhibit the growth of H8 cells, and the combination of salvianolic acid A (its active component) and oxysophoridine has a synergistic inhibitory effect on H8 cells and may be a potential treatment for cervical intraepithelial neoplasia.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Leng

Kan

et al. 2022

Molecules

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“…The solvent system selection is the most important step for a successful PZRCCC procedure. Ideal partition coefficient in both basic (K base ≫ 1) and acidic (K acid ≪ 1) and good solubility of the sample in the solvent system are essential for a suitable solvent system [33][34][35][36][37]. Based on the optimization of LLE solvent systems, n-hexane/ethyl acetate/methanol/water at volume ratios of 7:3:7:3 and 8:4:8:4 were tested, it was found the systems could not give small enough K acid , indicating the targets could not distribute well in aqueous phase when the pH was adjusted lower than 2.…”

Section: Selection Of Two-phase Solvent Systemmentioning

confidence: 99%

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

et al. 2020

J of Separation Science

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This study presents an efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic acids from natural products. First, an acid or base modified two‐phase solvent system with maximum or minimum partition coefficient was developed for the liquid‐liquid extraction of the crude extract. As a result, alkaloids or organic acids could be selectively enriched in the upper or lower phase. Then pH‐zone‐refining counter‐current chromatography was employed to separate and purify the selectively enriched alkaloids or organic acids efficiently. The selective enrichment and separation of five bufadienolide from toad venom of Bufo marinus was used as an example to show the advantage of this strategy. As a result, 759 mg of selectively enriched bufadienolide was obtained from 2 g of crude extract and the total content of five targets was increased from 14.64 to 83%. A total of 31 mg of marinobufagin‐3‐adipoyl‐l‐arginine, 42 mg of telocinobufagin‐3‐pimeloyl‐l‐arginine, 51 mg of telocinobufagin‐3‐suberoyl‐l‐arginine, 132 mg of marinobufagin‐3‐suberoyl‐l‐arginine, and 57 mg of bufalin‐3‐suberoyl‐l‐arginine were all simultaneously separated from 500 mg of selectively enriched sample, with the purity of 92.4, 97.5, 90.3, 92.1, and 92.8%, respectively.

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“…The interconversion of these compounds has been studied by various authors. Li et al [ 21 ] with the review of previous studies have outlined degradation processes in plant tissues and aqueous extracts where salvianolic acid A (SA-A, 22 ) is shown as the degradation product of SA-B among others ( Figure 2 ). Other studies have further shown that such transformation can readily takes place under high temperature, high pressure and high humidity environments in aqueous media [ 22 ].…”

Section: Natural Occurrence and Biosynthesis Of Rosmarinic And Salmentioning

confidence: 99%

Molecular Pharmacology of Rosmarinic and Salvianolic Acids: Potential Seeds for Alzheimer’s and Vascular Dementia Drugs

Habtemariam

2018

IJMS

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Both caffeic acid and 3,4-dihydroxyphenyllactic acid (danshensu) are synthesized through two distinct routs of the shikimic acid biosynthesis pathway. In many plants, especially the rosemary and sage family of Lamiaceae, these two compounds are joined through an ester linkage to form rosmarinic acid (RA). A further structural diversity of RA derivatives in some plants such as Salvia miltiorrhiza Bunge is a form of RA dimer, salvianolic acid-B (SA-B), that further give rise to diverse salvianolic acid derivatives. This review provides a comprehensive perspective on the chemistry and pharmacology of these compounds related to their potential therapeutic applications to dementia. The two common causes of dementia, Alzheimer’s disease (AD) and stroke, are employed to scrutinize the effects of these compounds in vitro and in animal models of dementia. Key pharmacological mechanisms beyond the common antioxidant and anti-inflammatory effects of polyphenols are highlighted with emphasis given to amyloid beta (Aβ) pathologies among others and neuronal regeneration from stem cells.

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Preparation of salvianolic acid A by the degradation reaction of salvianolic acid B in subcritical water integrated with pH-zone-refining counter-current chromatography

Cited by 14 publications

References 34 publications

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

An efficient strategy based on liquid‐liquid extraction and pH‐zone‐refining counter‐current chromatography for selective enrichment, separation, and purification of alkaloids and organic Acids from natural products

Molecular Pharmacology of Rosmarinic and Salvianolic Acids: Potential Seeds for Alzheimer’s and Vascular Dementia Drugs

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