Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Abstract

The effective treatment of cervical intraepithelial neoplasia (CIN) can prevent cervical cancer. Salvia miltiorrhiza is a medicinal and health-promoting plant. To identify a potential treatment for CIN, the effect of S. miltiorrhiza extract and its active components on immortalized cervical epithelial cells was studied in vitro. The H8 cell was used as a CIN model. We found that S. miltiorrhiza extract effectively inhibited H8 cells through the CCK8 method. An HPLC–MS analysis revealed that S. miltiorrhiza extract contained salvianolic acid H, salvianolic acid A, salvianolic acid B, monomethyl lithospermate, 9′′′-methyl lithospermate B, and 9′′′-methyl lithospermate B/isomer. Salvianolic acid A had the best inhibitory effect on H8 cells with an IC50 value of 5.74 ± 0.63 μM. We also found that the combination of salvianolic acid A and oxysophoridine had a synergistic inhibitory effect on H8 cells at molar ratios of 4:1, 2:1, 1:1, 1:2, and 1:4, with salvianolic acid A/oxysophoridine = 1:2 having the best synergistic effect. Using Hoechst33342, flow cytometry, and Western blotting analysis, we found that the combination of salvianolic acid A and oxysophoridine can induce programmed apoptosis of H8 cells and block the cell cycle in the G2/M phase, which was correlated with decreased cyclinB1 and CDK1 protein levels. In conclusion, S. miltiorrhiza extract can inhibit the growth of H8 cells, and the combination of salvianolic acid A (its active component) and oxysophoridine has a synergistic inhibitory effect on H8 cells and may be a potential treatment for cervical intraepithelial neoplasia.

Research progress on the mechanism of action of Salvia miltiorrhiza and its components in the treatment of endometrial carcinoma

“…In addition, salvianolic acid A can inhibit thymidine and uridine transport of ascites cancer cells, enhance the cytotoxicity of 5-fluorouracil, mitomycin C and methotrexate on KB cells and liver cancer BEL-7402 cells, and enhance the anti-tumor effect of chemotherapy drugs [17]. In terms of gynecological tumors, Leng et al [18] showed that salvianolic acid A combined with sophordine oxide could cooperatively regulate the cell cycle of cervical epithelial immortalized cells (H8), inhibit the expression of Bcl-2 protein, elevate the expression of Bax and Cleaved Caspase-3 protein, block H8 cells in the G2/M stage, and impede the proliferation of H8 cells. Promote their apoptosis.…”

Lignans from Mosla scabra Ameliorated Influenza A Virus-Induced Pneumonia via Inhibiting Macrophage Activation

“…e preparation and isolation of the lignans from M. scabra were employed as previous studies reported [20][21][22]. About 15 kg of aerial parts of the plant was extracted with 30 L of 95% ethanol 2 times for 24 h by using the heating reflux method.…”

Recent developments in Salvia miltiorrhiza polysaccharides: Isolation, purification, structural characteristics and biological activities

“…With more and more chemical and pharmacological researches on Salvia miltiorrhiza, researchers have found that Salvia miltiorrhiza exhibit diverse biological functions, such as anti-tumor, anti-oxidative stress, antithrombosis, anti-inflammatory, anti-liver fibrosis and antidiabetes activities (Guo et al, 2014;Zhou et al, 2014;Jiang et al, 2019;Petitjean et al, 2022). These effects are attributed to its wide range of bioactive ingredients, including tanshinone I, tanshinone IIA, tanshinone, procatechualdehyde, polysaccharide, salvianolic acids and other chemical substances (Jia et al, 2019;Kapalavavi et al, 2021;Leng et al, 2022).…”