Preparation of pullulan-g-poly(L-lysine) and it’s evaluation as a gene carrier

Pullulan and pullulan derivatives as promising biomolecules for drug and gene targeting

“…8). These modified nanoparticles show negligible cytotoxicity and are safe for gene delivery (Park et al, 2012).…”

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

“…were used as vector backbones to create polymeric vectors (Sherly, Rekha, & Harikrishnan, 2020). Due to the definitive effect of PUL-g-poly(L-lysine) (J. S. Park et al, 2012a), PUL-protamine (Priya et al, 2014) and succinylated PUL (Hyemin Kim & Na, 2010) as PUL derivatives on decreasing cytotoxicity. Table 7 summarizes the studies that applied PUL and its derivatives as the carrier for gene delivery applications.…”

Fabrication of a Controlled in Situ Forming Polypeptide Hydrogel with a Good Biological Compatibility and Shapeable Property

“…As can be seen from Figure 6a, the PLL-g-HPA copolymer did not display an obvious biotoxicity on the cells, and the cell viabilities were kept above 80% when the concentration of copolymer changes from 0.015 to 0.12 mg/mL, whereas PLL exhibited a moderate to high cytotoxicity with approximately 60% of cell viability. 42 Besides, the in vitro cytocompatibility of PEI as a positive control was also evaluated and compared with that of PLL-g-HPA; PEI showed a higher cytotoxicity. Additionally, the in vitro cytocompatibility of leaching solutions of the PLL-g-HPA hydrogels against L929 cells was shown in Figure 6b (3.4% gel, 5.1% gel, and 6.8% gel).…”