Pullulan and pullulan derivatives as promising biomolecules for drug and gene targeting

Singh, Ram Sarup; Kaur, Navpreet; Kennedy, John F.

doi:10.1016/j.carbpol.2015.01.032

Cited by 226 publications

(85 citation statements)

References 186 publications

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“…[20][21][22] Pullulan is a nonimmunogenic, nontoxic, noncarcinogenic, and nonmutagenic material that can be derivatized by chemical reactions with the hydroxyl groups of pyranose rings; therefore, it can be used for targeting drugs and gene delivery. 23 We have studied several characteristics of EPIloaded FA-conjugated pullulan acetate (FPA/EPI) NPs. 20,21 FA was conjugated to pullulan acetate to form the conjugated ligand and thereafter the pullulan acetate nanocarrier.…”

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confidence: 99%

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

Tang¹,

Chen²,

Jia³

et al. 2017

IJN

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The regular accumulation of nanoparticles in the liver makes them hepatotoxic and decreases the circulation time, thus reducing their therapeutic effect. Resolving this problem will be significant in improving bioavailability and reducing side effects. In this study, we reduced the phagocytosis of epirubicin (EPI)-loaded folic acid-conjugated pullulan acetate (FPA/EPI) nanoparticles by Kupffer cells (KCs) through internalization and nuclear factor kappa B (NF-kB) signal pathway inhibitors, thus allowing development of FPA/EPI nanoparticles as a nanodrug delivery system (NDDS) based on our previous study. FPA/EPI nanoparticles were prepared by the dialysis method. Rat KCs were preincubated with the following individual or compound inhibitors: chlorpromazine (CPZ), nystatin (NY), colchicine (Col), amiloride (AMR), and pyrrolidine dithiocarbamate (PDTC). Dose- and time-dependent cellular uptake effects of inhibitors on FPA/EPI nanoparticles were determined through fluorometry. The cytokine levels of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and IL-6 were tested in culture supernatants by bead-based multiplex flow cytometry. The uptake study demonstrated that inhibitors had an obvious inhibitory effect ( P <0.05 or P <0.01), with NY, AMR and Col all showing time-dependent inhibitory effects. PDTC + NY had the strongest inhibitory effect, with an uptake rate of 14.62%. The levels of the three proinflammatory cytokines were changed significantly by the compound inhibitors. TNF-α was significantly inhibited ( P <0.05 or P <0.01), but IL-1β and IL-6 showed smaller decreases. These results suggested that clathrin- and caveolae-mediated endocytosis were the main routes via which nanoparticles entered KCs and that the NF-kB signal pathway was very important too. In summary, multiple mechanisms, including clathrin- and caveolae-mediated endocytosis, contribute to cytokine production in macrophages following exposure to folic acid-conjugated pullulan acetate nanoparticles. Thus, the endocytosis inhibition strategy has great potential for improving therapy and reducing toxicity of an NDDS in the treatment of cancer.

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confidence: 99%

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

Tang¹,

Chen²,

Jia³

et al. 2017

IJN

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“…Other than viruses, cationic polymers, peptides and nanoparticles, and biopolymers such as chitosan, curdlan, hyaluronic acid, or pullulan were also investigated as siRNA delivery agents [89][90][91][92]. The rod-like nature of CNCs made them attractive bionanomaterials for gene delivery since rod-like nanoparticles were shown to be more rapidly internalized than spherical ones [93] and had a significant impact on gene transfection [94].…”

Section: Cationic Cncs As Sirna Delivery Agentsmentioning

confidence: 99%

Synthetic Strategies for the Fabrication of Cationic Surface-Modified Cellulose Nanocrystals

Sunasee

Hemraz

2018

Fibers

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Cellulose nanocrystals (CNCs) are renewable nanosized materials with exceptional physicochemical properties that continue to garner a high level of attention in both industry and academia for their potential high-end material applications. These rod-shaped CNCs are appealing due to their non-toxic, carbohydrate-based chemical structure, large surface area, and the presence of ample surface hydroxyl groups for chemical surface modifications. CNCs, generally prepared from sulfuric acid-mediated hydrolysis of native cellulose, display an anionic surface that has been exploited for a number of applications. However, several recent studies showed the importance of CNCs' surface charge reversal towards the design of functional cationic CNCs. Cationization of CNCs could further open up other innovative applications, in particular, bioapplications such as gene and drug delivery, vaccine adjuvants, and tissue engineering. This mini-review focuses mainly on the recent covalent synthetic methods for the design and fabrication of cationic CNCs as well as their potential bioapplications.

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“…Biomedical applications of pullulan include drug delivery, gene delivery [21,22], tissue engineering [23], vaccination [24,25], medical imaging [26], plasma expander [27], molecular chaperones [28], and film forming [29].…”

Section: Pullulan Is a Linear And Unbranched Exopolysaccharide Consismentioning

confidence: 99%

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

2018

J App Biol Biotech

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Pullulan is a random coil glucan polymer produced by Aureobasidium pullulans. It is one of the commercially promising biopolymers frequently used in pharmaceutical, food and cosmetics industries. The aim of present study was to isolate an efficient fungal strain producing melanin-free pullulan. The fungal strains were isolated from fresh and decaying leaves of plants and screened for pullulan production. Fungal isolate PUPY-06 was the best producer of pullulan (2.12%, w/v). Morphological studies revealed the oval and cylindrical shape of its cells, budding and presence of septate as well as pseudohyphae. Colonies of the isolate on agar plate cultures were beige-to-orange colored with smooth, mucoid, and butyrous texture. Morphological and biochemical characterization established the fungal isolate as Rhodosporidium sp. 18s rRNA sequencing, and phylogenetic analysis revealed the isolate to be Rhodosporidium paludigenum. Fourier-transform infrared spectroscopy and nuclear magnetic resonance of crude pullulan confirmed its structural characteristics. Maximum production of pullulan was achieved after 7 days of cultivation in shake-flask fermentations. Agitation mode of cultivation supported the higher production of pullulan in comparison to the stationary mode. R. paludigenum PUPY-06 was found as a novel pullulan-producing strain. This is a first report on pullulan production by R. paludigenum PUPY-06.

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Pullulan and pullulan derivatives as promising biomolecules for drug and gene targeting

Cited by 226 publications

References 186 publications

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

Effect of inhibitors of endocytosis and NF-kB signal pathway on folate-conjugated nanoparticle endocytosis by rat Kupffer cells

Synthetic Strategies for the Fabrication of Cationic Surface-Modified Cellulose Nanocrystals

Biochemical and molecular characterization of a new pullulan producer Rhodosporidium paludigenum PUPY-06

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