Preparation of porous silica spheres for column liquid

Unger, Klaus K.; Schick-Kalb, J.; Krebs, K.-F.

doi:10.1016/s0021-9673(00)97020-1

Cited by 64 publications

(8 citation statements)

References 2 publications

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“…The synthesis and/or size classification of silica particles is easily done to provide monodisperse particles of the desired pore size [22][23][24]. In addition, a large variety of silanes are available to modify silica's surface through silanization and make different bonded phases [25,26].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Luo

Carr

2008

Journal of Chromatography A

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A novel type of silica-based sulfonate-modified reversed phase ( − SO 3 -HC-C 8 ) has been synthesized; it is based on a newly developed acid stable "hyper-crosslinked" C 8 derivatized reversed phase, denoted HC-C 8 . The − SO 3 -HC-C 8 phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C 8 phase at different temperatures. The − SO 3 -HC-C 8 phases are only slightly less hydrophobic than the parent HC-C 8 phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown by Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the extraordinary high values of Snyder's column comparison function F s . In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity of these − SO 3 -HC-C 8 phases is greatly enhanced by its high hydrophobicity through a "hydrophobically assisted" ion-exchange retention process.

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Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Luo

Carr

2008

Journal of Chromatography A

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“…Reproducibility -Methylen selectivity -References Snyder et al, 1988;Sander and Wise, 1987;Buszewski, 1992Buszewski et al, 1998Buszewski, 1992Buszewski, 1992Bereznitski et al, 1996Unger, 1979Nawrocki, 1991;Buszewski, 1992Buszewski, 1992Murthy et al, 1985Bayer et al, 1986;Walters, 1987;Engelhardt and Jungheim, 1990;Buszewski, 1992;Galushko, 1993;Engelhardt et al, 1997;Albert, 2003;McCalley, 2003;Neue et al, 2003 Berendsen andde Galan, 1980;Sander and Wise, 1987;Buszewski, 1992;Kasturi et al, 1994Pines et al, 1973Murthy et al, 1985;Bayer et al, 1986;Sander and Wise, 1987;Buszewski, 1992;Albert, 2003Kimata et al, 1989Engelhardt and Jungheim, 1990;Galushko, 1991;Sander and Wise, 1995;Engelhardt et al, 1997;McCalley, 2003Kimata et al, 1989Engelhardt and Jungheim, 1990;…”

Section: Physico-chemical Methods For Stationary Phase Surface Characmentioning

confidence: 99%

Some remarks on characterization and application of stationary phases for RP-HPLC determination of biologically important compounds

Kowalska

Krupczyńska

Buszewski

2005

Biomed. Chromatogr.

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Biologically active compounds such as vitamins, steroids, nucleosides, peptides and proteins play a very important role in coordinating living organism functions. Determination of those substances is indispensable in pathogenesis. Their complex structure and physico-chemical properties cause many analytical problems. Chromatography is the most common technique used in pharmaceutical and biomedical analysis. The interaction between analyte and stationary phase plays a major role in the separation process. The structure of the packing has a significant influence on the results of the separation process. Various types of spectroscopic techniques, such as nuclear magnetic resonance spectroscopy, infrared spectroscopy, fluorescence spectroscopy and photoacoustic spectroscopy can be useful tools for the characterization of packings. Surface area measurements, elemental analysis, thermal analysis and microcalorimetric measurements are also helpful in this field. Part of the paper contains a description of chromatographic tests used for the determination of column properties. The description of the possibilities of surface characterization is not complete, but is focused on the most popular techniques and practical chromatographic tests. All the presented methods made possible the design and quality control of a new generation stationary phases, which are the future of high-performance liquid chromatography.

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“…For example, the solubility of racemates such as N-(1-naphthyl)ethyl-4-nitrobenzamide can be increased by a factor of 300-500 with chloroform as the mobile phase. 19 Based on the pioneering work of Stöber 20 and Unger 21 for the synthesis of silica particles with control of porosity and morphology, sol-gel chemistry provides a convenient route to chemically homogeneous organic/inorganic hybrid network materials with a uniformity of physical properties and high surface areas. [20][21][22] However, due to the immiscibility between hydrophobic cellulose derivatives and the hydrophilic silica framework, the synthesis of cellulose derived hybrid particles with a high surface area and controlled organic/inorganic ratio via sol-gel chemistry is challenging.…”

Section: Introductionmentioning

confidence: 99%

Preparation of porous cellulose 3,5-dimethylphenylcarbamate hybrid organosilica particles for chromatographic applications

et al. 2015

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Preparation of porous silica spheres for column liquid

Cited by 64 publications

References 2 publications

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases

Some remarks on characterization and application of stationary phases for RP-HPLC determination of biologically important compounds

Preparation of porous cellulose 3,5-dimethylphenylcarbamate hybrid organosilica particles for chromatographic applications

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