Preparation of Polyethylenimine-Hydroxyapatite and its Chromatographic Use

Abstract

Hydroxyapatite (HAp-CHT TypeII 40 mm) was coated with a stable layer of polyethylenimine (PEI). The resulting material was useful in column chromatography of acidic materials. A mixture of AMP, ADP, ATP, and adenosine could be resolved readily. The convenient separation of ovalbumin (one of the phosphoproteins) from other standard protein mixtures was observed.

Improved Preparation Method of Polyethylenimine-Hydroxyapatite and Its Chromatographic Properties

“…The PEI-HAp retained the ovalbumin (thick arrow) and a clear shoulder was observed, although the ovalbumin was not retained on the HAp. The result showed the same tendency as did the PEI-HAp synthesized by the previous method (11). Figure 5 shows the correlation of the retention time on HAp and on PEI-HAp.…”

“…Figure 8 shows the correlation between retention time on HAp and on PEI-HAp. These data show that nucleotide elution clearly depends on the number of the phosphate groups; chromatographic properties showed the same tendency as did the PEI-HAp synthesized by the previous method (11). The dotted lines in Fig.…”

“…In a previous article, we described a basic preparation method for PEIcoated HAp (PEI-HAp) and discussed its characteristics (11). In that method, however, the decantation process was required, for the removal of excess PEI, like a coating method for other materials (4)(5)(6)(7)(8)(9)11). The process removed part of the coating layer.…”