Preparation of Polyclonal Antibodies with Application for an Organophosphorus Pesticide Immunoassay

Yaneva, Marina; Ivanov, Yavor; Godjevargova, Tzonka

doi:10.1080/00032719.2016.1221417

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…Activation of the modified MNPs-NH2 is carried out with glutaraldehyde (GA) from Merck. Different antibodies were usedmonoclonal antitetracycline, anti-progesterone, anti-aflatoxin M1, anti-aflatoxin B1 and anti-enterotoxin A from Sigma-Aldrich, Germany, USA and polyclonal antipenicillin, anti-sulphonamide, anti-dichlorvos, antiparaoxon prepared in our laboratory (Yaneva et al 2017;Usleber et al 1998). For the preparation of fluorescent conjugates were used FITC, ATTO 488, ATTO 630, ATTO 590, and different analytes (Sigma-Aldrich, USA).…”

Section: Methodsmentioning

confidence: 99%

Magnetic nanoparticles based fluorescence immunoassay for food contaminants

Godjevargova

Ivanov

Atanasova

et al. 2019

FSAB

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Nanotechnology provides exciting new possibilities for advanced development of new analytical tools and instruments for bioanalytical applications. Magnetic nanoparticles (MNPs) have attracted much research interest in the past decade because they have good biocompatibility and can be readily separated from reaction mixtures with the aid of an external magnetic field. Heterogenic fluorescent immunoassays for determination of different analytes (antibiotics, pesticides, progesterone, aflatoxins, enterotoxins) using MNPs were developed. MNPs were prepared by thermal co-precipitation of Fe2+ and Fe3+ in aqueous solution. MNPs were functionalized with (3-aminopropyl) triethoxysilane. Two different analyzes were made for proving the successful modification of MNPs – FTIR and TEM. The corresponding antibodies were immobilized on modified MNPs. Then corresponding antigen - fluorescent dye conjugates were synthesized. The next step was purification of the resulting conjugates by gel filtration chromatography and proving them by UV-Vis and Fluorescence analyses. The 9 different separate competitive MNPs based immunoassays were developed – for penicillin, sulfonamide, tetracycline, progesterone, aflatoxin M1 and B1, enterotoxin A, paraoxon and dichlorvos. Then multiplex fluorescent immunoassay device based on magnetic nanoparticles for determination of penicillin, sulfonamide and tetracycline in milk sample was developed. The device ensured fast, selective and cheap antibiotic analysis. Very promising analytical characteristics for all three antibiotics were received - very low LODs and wide linear range. Practical applications: The multiplex fluorescent immunoassay device based on magnetic nanoparticles for determination of penicillin, sulfonamide and tetracycline in milk sample was developed. Device ensured fast, selective and cheap antibiotic analysis. Very promising analytical characteristics for all three antibiotics were received - very low LODs and wide linear range.

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Section: Methodsmentioning

confidence: 99%

Magnetic nanoparticles based fluorescence immunoassay for food contaminants

Godjevargova

Ivanov

Atanasova

et al. 2019

FSAB

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“…Broad-spectrum antibodies have also been used to develop immunoassays for high-throughput screening of pesticide and veterinary drug residues. An icELISA method was developed with polyclonal antibodies against dichlorvos and paraoxon [42]. The LODs and recovery values in real samples were determined with desirable results.…”

Section: Preparation and Application Of Broad-spectrum Antibodiesmentioning

confidence: 99%

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

et al. 2020

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The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.

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“…However, molecular tests have broader applications including use in epidemiological investigations, therapeutic strategies decisions and predicting the development of diseases. polymerase chain reaction (PCR): Several studies have shown that PCR is a specific and more sensitive method in the diagnosis of trypanosomosis in experimental as well as natural infections [22][23][24][25][26][27]. In actual fact, two periods of the trypanosome infection process must be distinguished; early in an infection, parasitological and PCR techniques show a very similar sensitivity (80%), but during the chronic phase of infection, parasitological examination exhibits a very low sensitivity and PCR is much more sensitive than the parasitological methods in such situation [22].…”

Section: Molecular Diagnosismentioning

confidence: 99%