Preparation of poly(ε-lysine) adsorbents and application to selective removal of lipopolysaccharides

Hirayama, Chuichi; Sakata, Masayo; Nakamura, Mariko; Ihara, Hirotaka; Kunitake, Masashi; Todokoro, Masami

doi:10.1016/s0378-4347(98)00479-4

Cited by 32 publications

(12 citation statements)

References 17 publications

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“…An e-PL polymer with 25-35 residues was discovered as a Dragendorff-positive substance from a strain of Streptomyces albulus No 346 (now available as S. albulus NBRC 14147) in culture filtrates Sakai 1977, 1981). e-PL is biodegradable, water-soluble and independent of oil-based resources, and has various but specific functions such as antimicrobial activities (Shima et al 1984;Hiraki 2000), an endotoxin-selective removal action (Hirayama et al 1999) or an antiobesity action by inhibiting intestinal absorption of dietary fat (Tsujita et al 2006). These functions are primarily ascribed to the cationic charge density of side-chain a-amino groups.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of nearly monodispersed poly(ε-l-lysine) in Streptomyces species

et al. 2007

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Poly(epsilon-L-lysine) (epsilon-PL) is a naturally occurring poly(amino acid) characterized by a unique structure linking epsilon-amino and carboxyl groups of L-lysine. Due to its various functions and its biodegradability and non-toxicity, the epsilon-PL polymer has attracted increasing attention in recent years. epsilon-PL is frequently found in various strains of Streptomyces sp. This review gives an up-to-date overview regarding the biosynthesis of epsilon-PL focussing mainly on results obtained from ten newly isolated producer strains, using the two-stage culture method of cell growth and epsilon-PL production cultures. The production of nearly monodispersed epsilon-PL is covered together with the development of epsilon-PL specific hydrolases and the release of synthesized epsilon-PL into the culture broth. From these results, coupled with the termination of polymerization through nucleophilic chain transfer, the biosynthetic mechanism of the polymer is discussed.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of nearly monodispersed poly(ε-l-lysine) in Streptomyces species

et al. 2007

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“…Similar phenomena have been presented by Hirayama et al They showed that the pK a of poly(⑀-lysine) resins decreased slightly with the introduction of chloromethyloxirane acting as a crosslinker. 13 From these results, it can be said that the pK a of the gel particles, which is composed of chitosan polymers, is lowered remarkably owing to gelation in the hydrophobic solvent, when compared with that of chitosan polymer.…”

Section: Effect Of the Gel Structure On The Adsorption Of The Surfactmentioning

confidence: 96%

Fundamental adsorption properties of chitosan gel particles prepared by suspension evaporation method

Igarashi

Nakano

2002

J of Applied Polymer Sci

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Chitosan gel particles were prepared by the suspension evaporation method, and their fundamental adsorption properties have been investigated. The chitosan gel particles were spherical in shape, 150 m in diameter, and had large macropores, about 2 m in diameter, formed by the polymer networks. The adsorption properties were evaluated from an interaction viewpoint using anionic surfactants having various numbers of carbon atoms, ethylbenzenesulfonate (EBS), octylbenzenesulfonate (OBS), and dodecylbenzenesulfonate (DBS). The pK a values of the amino groups within the gel particles were remarkably small compared with those of chitosan polymer. EBS, with its short hydrophobic chain, was adsorbed mainly by an electrostatic interaction in the pH range 3.0 to 6.0, where the amino groups were dissociated. On the contrary, the adsorption of OBS and DBS, with their relatively long hydrophobic chains, took place via an electrostatic interaction in the pH range 3.0 to 5.0, and then by a hydrophobic interaction in the presence of an electrostatic interaction in the pH range 5.0 to 8.0. We conclude that an electrostatic interaction is essential, even for the adsorption of surfactants having strong hydrophobic interactions.

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“…Analogous to PGA (see above), hydrogels with a high capacity for adsorption of water can be prepared from this polymer Kunioka and Choi 1995). Crosslinked PL can also be used as a cationic adsorbent in pharmaceutical applications (Hirayama et al 1999). Biotechnically produced polylysine is at present offered by the Chisso Corporation (Japan) (Hiraki and Suzuki 1999).…”

Section: Physiological Function and Commercial Significancementioning

confidence: 99%

Occurrence, functions and biosynthesis of polyamides in microorganisms and biotechnological production

Oppermann‐Sanio

Steinbüchel

2002

Naturwissenschaften

188

118

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Microorganisms are able to synthesize three different polyamides by enzymatic processes independently from ribosomal protein biosynthesis: poly(γ-Dglutamic acid), poly(ε-L-lysine) and multi-L-arginylpoly(L-aspartic acid) which is also referred to as cyanophycin. These polyamides, which occur mainly in Bacillus spp. (and only a few other eubacteria and the nematocysts of Cnidaria, in Streptomyces albulus or in cyanobacteria, respectively), have recently attracted considerable interest of the chemical industry and may be suitable for various applications. This review summarizes our current knowledge on the occurrence, biosynthesis, physiological functions, and biodegradation as well as on the properties and putative applications of these polyamides. Emphasis is placed on the enzymology of the polymerization and on the genes encoding the polymerizing enzymes, which have only recently become available for cyanophycin synthetases. Prospects for novel production processes, in particular for cyanophycin, are also presented.

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Preparation of poly(ε-lysine) adsorbents and application to selective removal of lipopolysaccharides

Cited by 32 publications

References 17 publications

Biosynthesis of nearly monodispersed poly(ε-l-lysine) in Streptomyces species

Biosynthesis of nearly monodispersed poly(ε-l-lysine) in Streptomyces species

Fundamental adsorption properties of chitosan gel particles prepared by suspension evaporation method

Occurrence, functions and biosynthesis of polyamides in microorganisms and biotechnological production

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