Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study

Abstract

Diabetes mellitus is one of the most common metabolic disorders. One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound. The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing. In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute. After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV−Vis spectra. The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis. The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects. According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.

Application of Electrospun Nanofiber Membrane in the Treatment of Diabetic Wounds

“…The cod liver oil loaded PLA/CS nanofiber membrane was prepared by uniaxial electrospinning, which was used to treat diabetic mice wounds. Wound experimental results of diabetic mice showed that after 14 d, the wound healing rate of the fiber group was 94.5%, which was much higher than that of the free cod liver oil group (40%) and the untreated diabetic wound group (13%) [97].…”

Sustained delivery of MMP-9 siRNA via thermosensitive hydrogel accelerates diabetic wound healing

“…With the promising siRNA uptake results described above, we then explored whether PM1(GT/siMMP-9 10 ) hydrogel can promote diabetic wound healing. The skin wound model in rats used in this study is a validated and widely used model to evaluate treatments for wound healing [ 36 , 37 ]. The quantitative analysis of the wound area over time showed that wounds of the non-diabetic group healed dramatically faster ( P < 0.05) than the diabetic group when both were treated with PBS (Fig.…”

Sustained Delivery of MMP-9 siRNA&nbsp;via Thermosensitive Hydrogel Accelerates Diabetic Wound Healing

“…PM1(GT/siMMP-9 10 ) hydrogel reduces MMP-9 expression and promotes wound healing With the promising siRNA uptake results described above, we then explored whether PM1(GT/siMMP-9 10 ) hydrogel can promote diabetic wound healing. The skin wound model in rats used in this study is a validated and widely used model to evaluate treatments for wound healing [34,35]. The quantitative analysis of the wound area over time showed that wounds of the non-diabetic group healed dramatically faster (P < 0.05) than the diabetic group when both were treated with PBS (Fig.…”