Preparation of phase 1Q fever antigen suitable for vaccine use

Spicer, D S; DeSanctis, Armand N.

doi:10.1128/aem.32.1.85-88.1976

Cited by 13 publications

(2 citation statements)

References 12 publications

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“…Antigens and immunizations. The immunizing and test antigens were a Formalin-inactivated phase I Henzerling strain vaccine prepared for human use (6), a cobalt-irradiated phase I Ohio strain, the soluble component obtained by extraction with trichloroacetic acid (TCA) of phase I Henzerling strain rickettsiae (4), the residue of such extraction (5), and the extract and residue of treatment of Ohio strain rickettsiae with chloroform-methanol (7). TCA fractions were kindly supplied by Ralph Wachter.…”

Section: Methodsmentioning

confidence: 99%

Dermal granulomatous hypersensitivity in Q fever: comparative studies of the granulomatous potential of whole cells of Coxiella burnetii phase I and subfractions

Ascher¹,

Williams²,

Berman³

1983

Infect Immun

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Dermal granulomatous reactivity to Q fever antigens in guinea pigs has been described as a model for vaccine reactions seen in previously sensitized humans. This model has now been applied to study the ability of subfractions of Coxiella burnetii to produce granulomas. Q fever organisms in phase I, trichloroacetic acid-soluble and-insoluble fractions, and the extract and residue of chloroformmethanol extraction were tested for their relative ability to elicit and immunize for dermal granulomatous reactions and specific lymphocyte proliferative responses in guinea pigs. The results suggest that a determinant(s) causing granulomas can be removed by chloroform-methanol extraction of phase I whole cells. The chloroform-methanol residue elicited strong delayed-type hypersensitivity without subsequent granuloma formation. The chloroform-methanol residue appears to possess a determinant(s) for lymphocyte stimulation equivalent to that of whole phase I organisms.

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Section: Methodsmentioning

confidence: 99%

Dermal granulomatous hypersensitivity in Q fever: comparative studies of the granulomatous potential of whole cells of Coxiella burnetii phase I and subfractions

Ascher¹,

Williams²,

Berman³

1983

Infect Immun

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“…Vaccination. The vaccine consisted of purified, Formalin-inactivated, particulate, Henzerling strain phase I C. burnetii (NDBR 105, lot 4) produced by the Merrell National Laboratories, Swiftwater, Pa. (22). Guinea pigs were vaccinated with 30,g of antigen, a dose reported by Fiset (6) to evoke humoral antibody production and to protect humans against an aerosol challenge with phase I rickettsiae 10 months later.…”

mentioning

confidence: 99%

Cell-mediated immune responses of guinea pigs to an inactivated phase I Coxiella burnetii vaccine

Kishimoto¹,

Johnson²,

Kenyon³

et al. 1978

Infect Immun

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The ability of a killed phase I Coxiella burnetii vaccine to induce cell-mediated immune responses in guinea pigs was studied. Cell-mediated immune responses were assessed by the inhibition of macrophage migration and lymphocyte transformation assays. The macrophage migration response occurred rapidly and was detected at high levels, but was relatively short-lived. In contrast, the lymphocyte transfornation response developed more slowly, and persisted for a longer period. The vaccine, given in a single dose or in two doses 1 week apart, protected guinea pigs from a subsequent virulent challenge.

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Biodefense and Special Pathogen Vaccines

Pittman¹,

Plotkin²

2018

Plotkin's Vaccines

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Preparation of phase 1Q fever antigen suitable for vaccine use

Cited by 13 publications

References 12 publications

Dermal granulomatous hypersensitivity in Q fever: comparative studies of the granulomatous potential of whole cells of Coxiella burnetii phase I and subfractions

Dermal granulomatous hypersensitivity in Q fever: comparative studies of the granulomatous potential of whole cells of Coxiella burnetii phase I and subfractions

Cell-mediated immune responses of guinea pigs to an inactivated phase I Coxiella burnetii vaccine

Biodefense and Special Pathogen Vaccines

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