Preparation of peroxidase and phenolics using discarded sweet potato old stems

Liu, Yang; Xi, Yi; Luo, Xiang-Yu; Ni, He; Li, Haihang

doi:10.1038/s41598-019-40568-9

Cited by 17 publications

(12 citation statements)

References 31 publications

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“…Although chemical synthesis could allow preparation of large quantities of pure TFs for biological assays, previous research on TFs focused on using mixtures because of the low abundance and challenging purification procedure of TFs . Until now, crude POD enzymes were still purified from potato to transform tea catechins into TFs . Similar products of (−)-epicatechin and (−)-epigallocatechin have been produced through treatment with homogenates of 62 plants belonging to 49 families.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolic Profiling Unveiled Roles of Peroxidases in Theaflavin Production in Black Tea Processing and Determination of Tea Processing Suitability

Zhang

Yang

Cui

et al. 2020

J. Agric. Food Chem.

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Theaflavins (TFs) are generated by endogenous polyphenol oxidase (PPO)- and peroxidase (POD)-catalyzed catechins oxidation during black tea processing, which needs to be well-controlled to obtain a proper TFs/thearubigins (TRs) ratio for better quality. Not all leaves from any tea plant cultivars or varieties are suitable for making high-quality black teas, regardless of the processing techniques. The mechanisms underlying TFs formation and the main factors determining the tea leaf processing suitability are not fully understood. We here integrated transcriptome and metabolite profiling of tea leaves to unveil how enzymes or metabolites in leaves are changed during black tea processing. The information enabled us to identify several PPO and POD genes potentially involved in tea processing for TF production. We characterized a POD gene, whose recombinant enzyme showed TF creation activity. The capacity for POD-catalyzed TF production could be used as a molecular marker for breeding tea plant varieties suitable for high-quality black tea production.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolic Profiling Unveiled Roles of Peroxidases in Theaflavin Production in Black Tea Processing and Determination of Tea Processing Suitability

Zhang

Yang

Cui

et al. 2020

J. Agric. Food Chem.

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“…After the before and after incubation absorbance values were obtained, the enzyme activity was determined by the following formula (Yang et al, 2019):…”

Section: Analysis Of Peroxidase Levelmentioning

confidence: 99%

Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp.

Wulan

Wibowo

Joko

et al. 2022

JPTI

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Plant Growth Promoting Rhizobacteria has been known for its ability to induce plant resistance on shallot against twisted disease. Its ability as a bioprotectant agent is estimated to be comparable to the efficacy of Trichoderma which is currently widely used as a biological control agent.. This study aims to determine the content of jasmonic acid, salicylic acid, peroxidase, and disease suppression in shallot by application of Bacillus velezensis B-27, Bacillus cereus RC76, and application with combination of both rhizobacteria. The application was carried out with tuber dipping for 30 minutes in each treatment with a bacterial density of 108 CFU mL-1. Application using Trichoderma was used as the comparison treatment, and the control plot was not given any treatment. Pathogen inoculation was carried out simultaneously as planting using Fusarium acutatum with a spore density of 106 CFU mL-1. The jasmonic and salicylic acids content was measured using the High-Performance Liquid Chromatography method, and the peroxidase content was determined using the spectrophotometric method. Disease suppression was measured at 10-day intervals. The results showed that treatment with Bacillus cereus RC76 increased jasmonic and salicylic acid levels, while application with Bacillus velezensis B-27 showed the highest level of peroxidase. Treatments with Bacillus spp. were able to suppress twisted disease by 72.2% to 100%. This study demonstrated that application Bacillus spp. suppressed twisted disease on shallot and increased the jasmonic and salicylic acid content as induced resistance mechanism against pathogens.

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“…rapa Barkant), obtained from a local market, which will be ready to be applied as a catalyst in biotransformation reactions. The turnip root was chosen due to availability, but the protocol described can be used for other peroxidase sources [25][26][27][28][29].…”

Section: Experimental Designmentioning

confidence: 99%

A Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extract

Rosa

Barreto

Pinto

et al. 2020

MPs

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Recently there is a great social expectation that scientists should produce more sustainable and environmentally friendly chemical processes. Within this necessity, biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials. In this work, we propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root. The protocol described includes (1) the preparation of the enzymatic extract, (2) the procedure for the assessment of the more favorable working parameters (temperature, pH) and (3) the methodology for the application of the extract as the catalyst for biotransformation reactions. We anticipate that the protocol in this research will provide a simple way for obtaining an enzymatic extract which can operate efficiently under mild conditions and can effectively catalyze the biotransformation of simple phenols.

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Preparation of peroxidase and phenolics using discarded sweet potato old stems

Cited by 17 publications

References 31 publications

Transcriptome and Metabolic Profiling Unveiled Roles of Peroxidases in Theaflavin Production in Black Tea Processing and Determination of Tea Processing Suitability

Transcriptome and Metabolic Profiling Unveiled Roles of Peroxidases in Theaflavin Production in Black Tea Processing and Determination of Tea Processing Suitability

Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp.

A Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extract

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