A Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extract

Abstract

Recently there is a great social expectation that scientists should produce more sustainable and environmentally friendly chemical processes. Within this necessity, biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials. In this work, we propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root. The protocol described includes (1) the preparation of the enzymatic extract, (2) the procedure for the assessment of the more favorable working parameters (temperature, pH) and (3) the methodology for the application of the extract as the catalyst for biotransformation reactions. We anticipate that the protocol in this research will provide a simple way for obtaining an enzymatic extract which can operate efficiently under mild conditions and can effectively catalyze the biotransformation of simple phenols.

Extraction, Purification and Characterization of Peroxidase from Okra (Abelmoschus esculentus)

“…It was mentioned [7,20], that the optimum pH for the activity of peroxidase extracted from ginger (Zingiber officinale) and medicinal plant Artocarpus lakoocha was 6. Whereas, [21], mentioned that the optimal pH for the turnip roots (Brassica rapa var. rapa Barkant) peroxidase was 4.…”

“…The enzymatic activity decreases due to a change in the nature of the enzyme or the substrate or both, which in turn leads to a decrease in enzyme activity and reaction rate. reaction decreases, and the heat may affect the base material itself and become not subject to the enzyme [20][21][22].…”

Bioremoval and Detoxification of the Anticancer Drug Mitoxantrone Using Immobilized Crude Versatile Peroxidase (icVP/Ba) Bjerkandera adusta CCBAS 930

“…Crude or partially purified ligninolytic enzymes can be used as low-cost alternatives in environmental applications [ 30 ]. Moreover, crude enzymes are often more stable than purified enzymes due to the presence of a mixture of components that confer protection against excessive oxidation and denaturation [ 31 ]. Immobilization methods are applied to improve the stability and recyclability of cells or biocatalysts compared to free cells or enzymes.…”