Preparation of performed films with predeterminable hole size distributions

Baumeister, Wolfgang; Seredynski, J.

doi:10.1016/0047-7206(76)90017-0

Cited by 12 publications

(6 citation statements)

References 6 publications

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“…The principal difficulty which both I and a coworker had encountered independently, and on separate occasions, dealt with achieving thorough emulsification of the glycerol in the nonpolar solvent. This had presumably been accomplished (Baumeister et al 1976) by ultrasonication but in our hands the step of ultrasonication merely caused persistent aggregation of the glycerol into buoyant, macroscopic white spheres rather than complete dispersal as claimed in the literature.…”

Section: Microgrid Mediods Developed For This Worksupporting

confidence: 60%

Improved methods for high resolution electron microscopy

Taylor¹

1987

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Existing methods of making support films for high resolution transmission electron microscopy are investigated and novel methods are developed. Existing methods of fabricating fenestrated, metal reinforced specimen supports (microgrids) are evaluated for their potential to reduce beam induced movement of monolamellar crystals of C44H90 paraffin supported on thin carbon films. Improved methods of producing hydrophobic Ill CHAPTER 4-RESULTS-MICROGRIDS 90 Ease of Production 90 Quality-Imaging Results 91 Carbon Films 95 Bonding Future Prospects 98 CHAPTER 5-INTRODUCTION-LABELING Site Specific Labeling of Bacteriorhodopsin CHAPTER6-BIOCHEMICALLABELING METHODS 107

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Section: Microgrid Mediods Developed For This Worksupporting

confidence: 60%

Improved methods for high resolution electron microscopy

Taylor¹

1987

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“…The particulate matter filtered from water samples was treated with n-hexane solution of rubber cement for fixing all particles in the 0.4-200 μm diameter range to the substrate. Some sediment samples were prepared on Formvar film [7]. 10 mg of sediment material was suspended in 15 mL of chloroform solution containing 1.5% Formvar (Polaron, Watford, UK).…”

Section: Samples and Sample Preparationmentioning

confidence: 99%

Comparison of sediment pollution in the rivers of the Hungarian Upper Tisza Region using non-destructive analytical techniques

Osán

Török

Alföldy

et al. 2007

Spectrochimica Acta Part B: Atomic Spectroscopy

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The rivers in the Hungarian Upper Tisza Region are frequently polluted mainly due to mining activities in the catchment area. At the beginning of 2000, two major mining accidents occurred in the Romanian part of the catchment area due to the failure of a tailings dam releasing huge amounts of cyanide and heavy metals to the rivers. Surface sediment as well as water samples were collected at six sites in the years 2000-2003, from the northeast-Hungarian section of the Tisza, Szamos and Túr rivers. The sediment pollution of the rivers was compared based on measurements of bulk material and selected single particles, in order to relate the observed compositions and chemical states of metals to the possible sources and weathering of pollution. Non-destructive X-ray analytical methods were applied in order to obtain different kinds of information from the same samples or particles. In order to identify the pollution sources, their magnitude and fate, complementary analyses were carried out. Heterogeneous particulate samples were analyzed from a large geographical territory and a 4-year time period. Individual particles were analyzed only from the "hot" samples that showed elevated concentrations of heavy metals. Particles that were classified as anthropogenic were finally analyzed to identify trace concentrations and chemical states of heavy metals.Although the Tisza river was affected by water pollution due to the two major mining accidents at the beginning of 2000, the concentration of heavy metals in sediments decreased to the mineral background level 1 year after the pollution event. In the tributaries Szamos and Túr, however, no significant decrease of the heavy metal concentrations was observed in the recent years, indicating a continuous pollution. Among the water suspended particles collected from river Túr, fibers of unknown origin were observed by electron microscopy; these particles were aluminosilicates enriched in Zn and Mn. Cd was also concentrated in this particle type. Zn in the sulfide form was observed by microbeam X-ray absorption near-edge structure analysis only in the samples collected from river Tisza in 2000, while this metal was connected to carbonates and silicates in the other samples. The dissolved Zn content of the river water and the chemical state of Zn in the fibers collected from river Túr indicate that the heavy metal pollution arrived to the Hungarian section originally mostly in the dissolved phase. The accidental heavy metal pollution of River Tisza, however, was in the form of microscopic metal sulfide particles.

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“…The type of film depends on the foreseen application. [6] A two-layer film made of a carbon-coated plastic polymer is often employed for negative stain EM (Figure 1A), while grids covered by a perforated lacey [7] or holey film (Figure 1b), usually made of carbon or gold, [8][9][10] have become commonplace in cryo-EM. These perforated grids can hold an additional thin layer of continuous carbon or graphene if required.…”

Section: Principles and Conventional Methods Of Em Grid Preparationmentioning

confidence: 99%

Miniaturizing EM Sample Preparation: Opportunities, Challenges, and “Visual Proteomics”

et al. 2018

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This review compares and discusses conventional versus miniaturized specimen preparation methods for transmission electron microscopy (TEM). The progress brought by direct electron detector cameras, software developments and automation have transformed transmission cryo-electron microscopy (cryo-EM) and made it an invaluable high-resolution structural analysis tool. In contrast, EM specimen preparation has seen very little progress in the last decades and is now one of the main bottlenecks in cryo-EM. Here, we discuss the challenges faced by specimen preparation for single particle EM, highlight current developments, and show the opportunities resulting from the advanced miniaturized and microfluidic sample grid preparation methods described, such as visual proteomics and time-resolved cryo-EM studies.

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Preparation of performed films with predeterminable hole size distributions

Cited by 12 publications

References 6 publications

Improved methods for high resolution electron microscopy

Improved methods for high resolution electron microscopy

Comparison of sediment pollution in the rivers of the Hungarian Upper Tisza Region using non-destructive analytical techniques

Miniaturizing EM Sample Preparation: Opportunities, Challenges, and “Visual Proteomics”

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