Preparation of Nano Zinc Particles and Evaluation of Its Application in Mouse Myocardial Infarction Model

Abstract

Nanometer zinc particles were synthesized by orthogonal test with manganese chloride, iron chloride and zinc sulfate as raw materials and NaOH as coprecipitating agent. The optimum synthesis conditions of coprecipitation method were obtained and the samples were characterized by various means. In this experiment, the SV, EF, FS, lvaws, lvawd, lvpws and lvpwd of left ventricle in mice with myocardial infarction were decreased, while the LVEDd, lveds and lvevs were increased in the environment exposed to ultrafine zinc nanoparticles, which proved that exposure to ultrafine zinc nanoparticles could lead to the enlargement of left ventricle, the thinning of ventricular wall, and the decrease of cardiac systolic and diastolic function. Further study on the heart tissue sections showed that the normal left ventricular myocardium of mice exposed to ultrafine zinc nanoparticles decreased, apoptotic cells increased, collagen content increased significantly, and myocardial fibrosis intensified. At the same time, WGA staining results of myocardial cell membrane showed that inhalation of ultra-fine nano zinc particles increased the size of myocardial infarction cells and disordered cell arrangement, which further proved that inhalation of ultra-fine nano zinc particles accelerated left ventricular pathological remodeling. The results of this study prove that the ultra-fine zinc nanoparticles in the air play an important role in the structural remodeling of myocardial infarction heart, and provide a theoretical basis for formulating targeted policies to control air pollution.