Preparation of Multifunctional Polysaccharide Microcontainers for Lipophilic Bioactive Agents

Bоrodinа, Tatyana N.; Grigoriev, D. O.; Carillo, Maria Antonietta; Hartmann, J.; Moehwald, Helmuth; Shchukin, Dmitry G.

doi:10.1021/am406039r

Cited by 32 publications

(39 citation statements)

References 30 publications

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“…Sonication has been widely used for the synthesis and/or dispersion of a wide range of organic and inorganic particles or filaments at the nano-or mesoscale. 53,54,55 It was also implemented for the functionalization of materials 56 The impact of the molecular structure of the polymer on the compatibility with the ZIF-8 NPs was also investigated by using the PIM-EA-TB polymer (see Scheme 1). Similar results were obtained with the ZIF-8/PIM-EA-TB system for a ZIF-8 content ranging from 50, 30, and 20 wt%, showing the coexistence of PIM-EA-TB particles and compact aggregates of ZIF-8 NPs with a diameter of a few hundreds of nanometers (see Figure S16).…”

Section:  Results and Discussionmentioning

confidence: 99%

Toward an Understanding of the Microstructure and Interfacial Properties of PIMs/ZIF-8 Mixed Matrix Membranes

Benzaqui

Semino

Menguy

et al. 2016

ACS Appl. Mater. Interfaces

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A study integrating advanced experimental and modeling tools was undertaken to characterize the microstructural and interfacial properties of mixed matrix membranes (MMMs) composed of the zeolitic imidazolate framework ZIF-8 nanoparticles (NPs) and two polymers of intrinsic microporosity (PIM-1 and PIM-EA-TB). Analysis probed both the initial ZIF-8/PIM-1 colloidal suspensions and the final hybrid membranes. By combination of dynamic light scattering (DLS) and transmission electron microscopy (TEM) analytical and imaging techniques with small-angle X-ray scattering (SAXS), the colloidal suspensions were shown to consist mainly of two distinct kinds of particles, namely, polymer aggregates of about 200 nm in diameter and densely packed ZIF-8-NP aggregates of a few 100 nm in diameter with a 3 nm thick polymer top-layer. Such aggregates are likely to impart the granular texture of ZIF-8/PIMs MMMs as shown by SEM-XEDS analysis. At the molecular scale, modeling studies showed that the surface coverage of ZIF-8 NPs by both polymers appears not to be optimal with the presence of microvoids at the interfaces that indicates only a moderate compatibility between the polymer and ZIF-8. This study shows that the microstructure of MMMs results from a complex interplay between the ZIF-8/PIM compatibility, solvent, surface chemistry of the ZIF-8 NPs, and the physicochemical properties of the polymers such as molecular structure and rigidity.

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Section:  Results and Discussionmentioning

confidence: 99%

Toward an Understanding of the Microstructure and Interfacial Properties of PIMs/ZIF-8 Mixed Matrix Membranes

Benzaqui

Semino

Menguy

et al. 2016

ACS Appl. Mater. Interfaces

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“…The mechanism of the microcontainers formation has been described in detail earlier. 8 As shown in Table 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 loaded MC slightly increased without distinct size variability. A modification of the MC surface by a PLL polycation adsorption resulted in surface charge changing, namely a negative charge for a positive one.…”

Section: Preparation and Characterization Of Microcontainersmentioning

confidence: 93%

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Akasov

Bоrodinа

Zaytseva

et al. 2015

ACS Appl. Mater. Interfaces

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High toxicity, poor selectivity, and severe side effects are major drawbacks of anticancer drugs. Various drug delivery systems could be proposed to overcome these limitations. The aim of this study was to fabricate polysaccharide microcontainers (MCs) loaded with thymoquinone (TQ) by a one-step ultrasonication technique and to study their cellular uptake and cytotoxicity in vitro. Two MC fractions with a mean size of 500 nm (MC-0.5) and 2 μM (MC-2) were prepared and characterized. Uptake of the MCs by mouse melanoma M-3 cells was evaluated in both 2D (monolayer culture) and 3D (multicellular tumor spheroids) models by confocal microscopy, flow cytometry, and fluorimetry. The higher cytotoxicity of the TQ-MC-0.5 sample than the TQ-MC-2 fraction was in good correlation with higher MC-0.5 accumulation in the cells. The MC-0.5 beads were more promising than the MC-2 particles because of a higher cellular uptake in both 2D and 3D models, an enhanced antitumor effect, and a lower nonspecific toxicity.

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“…To prevent complex formation, every polysaccharide solution was adjusted to pH 2 using hydrochloric acid before mixing. 20 Characterizations. To obtain scanning electron microscopy (SEM) images, HIPEs were placed onto a silicon wafer, freezedried, and sputtered with gold.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Sonochemically Synthesized Ultrastable High Internal Phase Emulsions via a Permanent Interfacial Layer

Tan

Lee

Pajoumshariati

et al. 2018

ACS Sustainable Chem. Eng.

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In this work, we demonstrate sonochemically synthesized high internal phase emulsions (HIPEs), which feature high stability due to a permanent layer at the oil−water interface that nearly any material can form as long as it can be cross-linked during acoustic cavitation. Compared to conventional HIPEs prepared by homogenization emulsification, these ultrasonically prepared HIPEs exhibit excellent performance, including high tolerance to environmental shock (e.g., pH and surfactant) and excellent processability, as well as the capacity to structure liquid oil and provide controlled delivery for bioactive compounds. By changing the acoustic intensity, we can also easily tune the morphology, rheological properties, porosity, and closed-/open-cell structure of the porous materials. Furthermore, this unique type of HIPE is potentially sustainable because it can be stabilized solely by natural materials, such as proteins and polysaccharides, even at 0.5 wt %, without introducing any additional surfactants or synthetic particles. These findings suggest this sonochemical technique holds promise as a versatile, scalable, and environmentally friendly route for synthesizing ultrastable and fully natural HIPEs. Additionally, the material performance shown herein provides a proof-of-concept for the design of multifunctional HIPEs in various applications.

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Preparation of Multifunctional Polysaccharide Microcontainers for Lipophilic Bioactive Agents

Cited by 32 publications

References 30 publications

Toward an Understanding of the Microstructure and Interfacial Properties of PIMs/ZIF-8 Mixed Matrix Membranes

Toward an Understanding of the Microstructure and Interfacial Properties of PIMs/ZIF-8 Mixed Matrix Membranes

Ultrasonically Assisted Polysaccharide Microcontainers for Delivery of Lipophilic Antitumor Drugs: Preparation and in Vitro Evaluation

Sonochemically Synthesized Ultrastable High Internal Phase Emulsions via a Permanent Interfacial Layer

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