Preparation of multicolor emitting carbon dots for HeLa cell imaging

Mehta, Vaibhavkumar N.; Jha, Sanjay Kumar; Singhal, R. K.; Kailasa, Suresh Kumar

doi:10.1039/c4nj00840e

Cited by 218 publications

(98 citation statements)

References 47 publications

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“…Furthermore, the C-dots show the maximum emission peak centered at 461 nm, when excited at 383 nm (Fig. 3a), with a full width at half maximum (FWHM) of 102 nm, which is similar to that of the carbon particles obtained from potato (Solanum tuberosum) β-cyclodextrin and alloyed quantum dots [30][31][32]. The FWHM of the C-dots is due to the narrower size distribution of the C-dots and the limited processes of radiative recombination of photo-generated carriers.…”

Section: Resultssupporting

confidence: 56%

Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice

et al. 2015

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In this paper, we have described a simple hydrothermal method for preparation of fluorescent carbon dots (C-dots) using Carica papaya juice as a precursor. The synthesized C-dots show emission peak at 461 nm with a quantum yield of 7.0 %. The biocompatible nature of C-dots was confirmed by a cytotoxicity assay on E. coli. The C-dots were used as fluorescent probes for imaging of bacterial (Bacillus subtilis) and fungal (Aspergillus aculeatus) cells and emitted green and red colors under different excitation wavelengths, which indicates that the C-dots can be used as a promising material for cell imaging.

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Section: Resultssupporting

confidence: 56%

Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice

et al. 2015

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“…Sachdev et al . reported a facile treatment of coriander leaves for preparing BCDs . Honey, black tea, egg white, papaya, goose feathers, and other diverse biomass are widely used to prepare carbon dots for iron ion detection .…”

Section: Applications Of Bcdsmentioning

confidence: 99%

Biomass‐Derived Carbon Dots and Their Applications

Meng

Bai

Wang

et al. 2019

Energy & Environ Materials

321

172

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Carbon dots (CDs) have received much attention due to their superior properties including water solubility, low toxicity, biocompatibility, small size, fluorescence, and ease of modification. The use of a more environmentally friendly method to prepare high‐quality CDs is still an urgent question waiting for solve. The use of renewable, inexpensive, and green biomass resources not only meets the urgent need for large‐scale synthesis biomass CDs (BCDs), but also promotes the development of sustainable applications. In this article, we summarize the representative methods for synthesizing BCDs in green and simple ways using biomass as a carbon source, including hydrothermal carbonization, and microwave, pyrolysis. The prepared BCDs have a uniform particle size distribution and a relatively high throughput, which provide a method to scale up industrial production. Moreover, the integration of specific optical properties, that is, tunable photoluminescence and up‐photoluminescence, has led to remarkable use in bioimaging, sensing, and drug delivery. But the current review is not particularly comprehensive for BCDs. Therefore, we now provide a review focusing on the synthesis, properties, and recent advances in BCDs in biosensing, bioimaging, optoelectronics, and catalytic applications.

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“…is the only thermal technique that has been applied to characterize C-dots [27,32,56,69,88,93,158,174,175]. In TGA measurement, the instrument continuously weighs a C-dots sample by coupling with FTIR or MS for gas analysis.…”

Section: Thermal Techniques Thermogravimetric Analysis (Tga)mentioning

confidence: 99%

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

Gong

Liu

et al. 2017

Journal of Nanomaterials

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Carbon nanodots (C-dots) are recently discovered fluorescent carbon nanoparticles with typical sizes of <10 nm. The C-dots have been reported to have excellent photophysical and chemical characteristics. In recent years, the advances in the development and improvement in C-dots synthesis, characterization, and applications are burgeoning. In this review, we introduce the most commonly used techniques for the characterization of C-dots. The characterization techniques for C-dots are briefly classified, described, and illustrated with applied examples. In addition, the analytical separation methods for C-dots (including electrophoresis, chromatography, density gradient centrifugation, differential centrifugation, solvent extraction, and dialysis) are included and discussed according to their analytical characteristics. The review concludes with an outlook towards the future developments in the characterization and the analytical separation of C-dots. The comprehensive overview of the characterization and the analytical separation techniques will safeguard people to use each technique more wisely.

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Preparation of multicolor emitting carbon dots for HeLa cell imaging

Abstract: Confocal laser microscopic images of HeLa cells using CDs as fluorescent probes.

Cited by 218 publications

References 47 publications

Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice

Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice

Biomass‐Derived Carbon Dots and Their Applications

Characterization and Analytical Separation of Fluorescent Carbon Nanodots

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