Preparation of monoclonal antibody and identification of two novel B cell epitopes to VP1 protein of porcine sapelovirus

Hao, Chenlin; Ren, Haojie; Wu, Xueping; Shu, Xiangli; Li, Zhaoyang; Hu, Yating; Zhang, Yucan; Zu, Shaopo; Yuan, Junhua; Zhang, Honglei; Hu, Hui

doi:10.1016/j.vetmic.2022.109593

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…Identifying B-cell epitopes in viral proteins holds significant meaning for vaccine development, clinical diagnosis and antibody production. [ 27 , 28 , 29 ]. Clearly defining epitopes targeted by monoclonal antibodies will greatly contribute to understanding antigen structure and advancing antiviral therapies [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can impair immunological function, stunt growth and decrease egg production in avian flocks. The capsid protein (P27) is an attractive candidate for ALV diagnostics. In the present study, a new hybridoma cell (1F8) stably secreting an anti-P27 monoclonal antibody (mAb) was developed. The mAb exhibited a high affinity constant (Ka) of 8.65 × 106.0 L/mol, and it could be used for the detection of ALV-A/B/J/K strains. Moreover, a total of eight truncated recombinant proteins and five synthetic polypeptides were utilized for the identification of the B-cell epitopes present on P27. The results revealed that 218IIKYVLDRQK227 was the minimal epitope recognized by 1F8, which had never been reported before. Additionally, the epitopes could strongly react with different ALV subgroup’s specific positive serum and had a complete homology among all the ALV subgroups strains. Finally, a new sandwich ELISA method was created for the detection of ALV antigens, demonstrating increased sensitivity compared to a commercially available ELISA kit. These results offer essential knowledge for further characterizing the antigenic composition of ALV P27 and will facilitate the development of diagnostic reagents for ALV.

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Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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“…To confrm the replication of PSV on LLC-PK and ST cells, IFA was conducted using PSV VP1 specifc monoclonal antibody (prepared in our laboratory) [27]. Te results indicated that the infectious-cells showed large numbers of IF-stained cells (Figures 1(f ) and 1(h)).…”

Section: Isolation and Propagation Of Psv In St And Llc-pk Cellsmentioning

confidence: 97%

Isolation and Characterization of Porcine Sapelovirus from the PDCoV-Positive Sample and Its Molecular Epidemiology in Henan Province, China

Zhang

et al. 2023

Transboundary and Emerging Diseases

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Porcine sapelovirus (PSV) is an emerging swine enteric virus that can cause various disorders including acute diarrhea, respiratory distress, reproductive failure, and polioencephalomyelitis in pigs. In this study, we isolated a PSV strain HNHB-01 from a clinical porcine deltacoronavirus- (PDCoV-) positive intestinal content of a diarrheic piglet. PSV was first identified using the small RNA deep sequencing and assembly, and further identified by the electron microscopic observation and the immunofluorescence assay. Subsequently, this virus was serially passaged in swine testis (ST) cells, and the complete genomics of PSV HNHB-01 passage 5 (P5), P30, P60, and P100 were sequenced and analyzed. 9 nucleotide mutations and 7 amino acid changes occurred in the PSV HNHB-01 P100 strain when compared with the PSV HNHB-01 P5. Pathogenicity investigation showed that orally inoculation of PSV HNHB-01 P30 could cause obvious clinical symptoms and had broad tissue tropism in 5-day-old piglets. Epidemiological investigation revealed that PSV infections and the coinfections of diarrhea coronaviruses were highly prevalent in swine herds. The complete genomes of 8 representative PSV epidemic strains were sequenced and analyzed. Phylogenetic analysis revealed that the PSV epidemic strains were closely related to other PSV reference strains that located in the Chinese clade. Furthermore, recombination analysis revealed that the recombination events were occurred in downstream of the 2C region in our sequenced PSV HNNY-02/CHN/2018 strain. Our results provided theoretical basis for future research studies of the pathogenic mechanism, evolutionary characteristics, and the development of vaccines against PSV.

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Preparation of monoclonal antibody and identification of two novel B cell epitopes to VP1 protein of porcine sapelovirus

Cited by 2 publications

References 33 publications

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Isolation and Characterization of Porcine Sapelovirus from the PDCoV-Positive Sample and Its Molecular Epidemiology in Henan Province, China

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