Preparation of molecularly imprinted polymers on the surface of magnetic carbon nanotubes with a pseudo template for rapid simultaneous extraction of four fluoroquinolones in egg samples

Abstract

Fluoroquinolones (FQs) have emerged as one of the most important class of antibiotics. Due to their low concentration in bio-matrix samples which contain a lot of interfering substances, the efficient solid phase extraction and accurate determination of FQs remain a challenge. In this paper, a new strategy for the isolation and enrichment of FQs from egg samples was obtained by molecularly imprinted polymers on the surface of magnetic carbon nanotubes (MCNTs@MIP), which not only can be collected and separated rapidly by an external magnetic field, but also have a high specific surface area, outstanding mechanical properties and specific recognition for FQs. MCNTs@MIP were prepared using ofloxacin as a pseudo template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The characteristics of the MCNTs@MIP were assessed by transmission electron microscopy (TEM), multipoint Brunauer-Emmett-Teller (BET) analysis, vibrating sample magnetometry (VSM), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. The results of the adsorption experiments not only demonstrated rapid dynamic adsorption but also showed a high selectivity toward FQs. An extraction method using MCNTs@MIP coupled with high performance liquid chromatography (HPLC) was developed for the determination of four FQs in egg samples. The recovery of four FQs ranged from 95.2% ± 3.2% to 100.7% ± 3.1% and the detection limits ranged from 0.25-0.40 ng g(-1). The results demonstrate that the proposed method based on pseudo template MCNTs@MIP is a promising approach for the preconcentration, purification, and simultaneous analysis of four FQs in bio-matrix samples.

Applications of magnetic surface imprinted materials for solid phase extraction of levofloxacin in serum samples

“…The preparation of MCNTs@MIPs was carried out according to our previous work (Xiao et al, 2013a(Xiao et al, , 2013b with minor change. LOFX (1.0 mmol) was dissolved in 10 mL DMSO, and 4.0 mmol MAA was added then followed by ultrasonication for 30 min to prepare preassembly solution.…”

Preparation of novel curcumin-imprinted polymers based on magnetic multi-walled carbon nanotubes for the rapid extraction of curcumin from ginger powder and kiwi fruit root

“…Furthermore, nanomaterials were also considered to combine with Fe 3 O 4 nanoparticles to obtain high specific surface area and improve the ductility of magnetic materials. Xiao et al listed several preparation methods for magnetic carbon nanotubes applied in synthesizing MMIPs . Tayyebeh Madrakian et al prepared an imprinted polymer based on magnetic multi‐walled carbon nanotubes (MMWNTs) using amidoamine as the functional monomers for determination of naproxen in human urine samples .…”

A selective molecularly imprinted polymer-carbon nanotube sensor for cotinine sensing

“…Other researchers have coated the MIP directly onto the walls of functionalized CNTs, and here, we cite several of the more recent reports on this technology. Fluoroquinolones were extracted from eggs using a poly(methacrylic acid) MIP on magnetic CNTs, and the magnetic properties were used to isolate the polymers (Xiao et al, 2013). Chlorpyrifos have been separated from aqueous solutions using vinyl-functionalized CNTs (Anirudhan and Alexander, 2013), and a similar separation for ribavirin from solution was shown to be effective using a polyacrylamide MIP coated onto nanotubes (Xu and Xu, 2012).…”