Preparation of methacrylate-based anion-exchange monolithic microbore column for chromatographic separation of DNA fragments and oligonucleotides

Sabarudin, Akhmad; Huang, Junfei; Shu, Shin; Sakagawa, Shinnosuke; Umemura, Tomonari

doi:10.1016/j.aca.2012.05.039

Cited by 41 publications

(38 citation statements)

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“…The poly-(GMA-co-EDMA) monolith was synthesized by in-situ copolymerization reaction inside a silicon steel tubing (1.02 mm i.d., 1/16 in o.d.) using the procedure described by Sabarudin, et al 18 A mixture of GMA, EDMA, 1-propanol, 1,4-butanediol, water, and AIBN (1% w/v of the total monomer amount) was homogenized using ultrasonication for 10 min and injected into a silanized silicon steel tubing by mean of a-5 mL syringe. Both ends column was sealed, and the polymerization proceeded in the oven at 60°C for 12 h. The resulted monolithic column was then washed with ethanol and water to remove residual reagents.…”

Section: Pretreatment Of Silicosteel Tubing and Preparation Of Ti 4+ mentioning

confidence: 99%

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"DEVELOPMENT OF Ti4+-IMMOBILIZED NANOPOROUS MONOLITHIC POLYMER FOR SELECTIVE SEPARATION AND DETECTION OF PHOSPHOPEPTIDES "

2018

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Phosphopeptides present in a large number of biological regulating mechanisms. It plays a significant role in gene regulation, eukaryotic signal transduction, and metabolic control in a cell. Abnormal phosphorylation can cause various diseases, including cancer. However, phosphoprotein abundances are very low, only 1-2%. In this work, the nanoporous monolith columns were developed for separation and detection of phosphopeptides in phosphoproteome analysis. The nanoporous monolith-based column is prepared inside a silicosteel tubing (100 x 1.02 mm i.d.) by insitu copolymerization reaction of glycidyl methacrylate (GMA) with ethylene dimethacrylate (EDMA). This monolith was further chemically modified by introducing aminomethyl phosphonic acid (AMPA) before immobilization of Ti 4+ ion (Ti 4+ -immobilized). The monolithic column properties, such as morphology, elemental analysis, surface area analysis, permeability and pore distribution were characterized in detail. Such a Ti 4+ -immobilized nanoporous monolith-based column with immobilization time 3 hours of TiCl4 without glutaraldehyde addition was further applied to separation and detection of phosphopeptides from digested proteins (β-casein and cytochrome-c), and tyrosine phosphorylated peptide samples. The result demonstrated that Ti 4+ -immobilized nanoporous monolith-based provide higher selectivity and efficiency for selective detection of phosphopeptides using liquid chromatography.

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Section: Pretreatment Of Silicosteel Tubing and Preparation Of Ti 4+ mentioning

confidence: 99%

“…Development of microbore column with inner diameter of SEPARATION 0.5 -1.5 mm has been reported. [18][19][20] This kind of column easily utilized to standard HPLC system without any modification. 19,21 In term of applicability, the benefit of microbore column can be used for analysis and also for preparative purposes.…”

Section: Introductionmentioning

confidence: 99%

"DEVELOPMENT OF Ti4+-IMMOBILIZED NANOPOROUS MONOLITHIC POLYMER FOR SELECTIVE SEPARATION AND DETECTION OF PHOSPHOPEPTIDES "

2018

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“…Sebagai media pemisahan, monolith telah banyak diaplikasikan sebagai pengganti kolom partikel konvensional yang selama ini digunakan dalam KCKT. Variasi dan aplikasi kolom monolith telah berkembang dengan pesat dalam dekade terakhir ini dan memainkan peranan yang sangat penting dalam ilmu kimia pemisahan [1,2].…”

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“…Penggunaan porositas dan ukuran pori yang dapat divariasi, monolith terbukti dapat berfungsi sebagai media pemisahan dalam kromatografi untuk pemisahan berbagai analit, mulai dari molekul berukuran kecil [3,[7][8], hingga makromolekul seperti peptida dan protein [9][10], dan juga oligodeoxythymidylic acids [2].…”

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Preparasi Kolom Microbore Monolith Berbasis Polistirena untuk Pemisahan Alkilbenzena Menggunakan Kromatografi Cair Kinerja Tinggi

Tasfiyati

Solandjari

Iftitah

et al. 2015

Nat-B

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Kata kunci : kolom monolith, microbore, polistirena, kromatografi cair fasa terbalik, alkilbenzena ABSTRACTIn this study, polystyrene-based monolith was developed as reversed phase column in high performance liquid chromatography (HPLC) for separation of small molecule sample (alkylbenzene). Polystyrene-based monolithic column was prepared in microbore silicosteel column (100 x 1.02 mm i.d.) by in-situ polymerization reaction using styrene as monomer, divinylbenzene as crosslinker, porogenic solvents, with the presence of radical initiator 2,2'-azobis(isobutyronitrile). The morphology of the monolithic column was studied by SEM. The properties of the monolithic column, such as permeability, mechanical stability, binding capacity and pore size distribution, were characterized in detail. From the characterization results of 9 monolith variation, monolith PS-DVB with %T 30, %C 50, using toluene:1-decanol (3:7 v/v) as porogenic solvent was found to be the ideal composition and gave the best separation of alkylbenzene. It has theoretical plate value (N) of 17260/m column, also has relatively equal proportion of flow-through pore and mesopores (47% and 44% respectively). The performance of monolithic column was also compared with commercially available monolithic column Proswift RP-4H. PS-DVB monolith prepared in this study shown its superiority over commercial one.Keywords : monolithic column, microbore, polystyrene, reversed phase liquid chromatography, alkylbenzene PENDAHULUANMonolith adalah material berpori kontinyu dengan struktur pori yang terdiri dari 3 jenis ukuran, yaitu micropore (<2 nm), mesopore (2-

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“…Microbore column can be easily connected to commercially available standard HPLC system with minor modification. Although requiring more attention to the uneven heating along the diameter of the column during the polymerization process, microbore columns combine the advantages of both capillary and conventional-size columns, that is low sample and reagent consumption, reduced waste, shorten analysis time, and enhanced sensitivity from the small amount of sample [13,28,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Malis

Tasfiyati

Iftitah

et al. 2015

J. Pure App. Chem. Res.

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A polymer monolithic column, poly-(methacrylatoethyltrimethylammonium chloride-covinylbenzyl chloride-co-ethylene dimethacrylate) or poly-(MATE-co-VBC-co-EDMA) was successfully prepared in the current study by one-step thermally initiated in situ polymerization, confined in a steel tubing of 0.5 mm i.d. and 1/16" o.d. The monoliths were prepared from methacrylatoethyltrimethylammonium chloride (MATE) and vinylbenzyl chloride (VBC) as monomer and ethylene dimethacrylate (EDMA) as crosslinker using a binary porogen system of 1-propanol and 1,4-butanediol. The inner wall of steel tubing was pretreated with 3-methacryloxypropyl-trimethoxysilane (MAPS). In order to obtain monolith with adequate column efficiency and low flow resistance, some parameters such as total monomer concentration (%T) and crosslinker concentration (%C) were optimized. The morphology of this monolith was assessed by scanning electron microscopy (SEM). The properties of the monolithic column, such as permeability, binding capacity, and pore size distribution were also characterized in detail. From the results of the characterization of all monolith variation, monolith with %T 30 %C 50 and %T 35 %C 50 give the best characteristic. These monoliths have high permeability, adequate molecular recognition sites (represented with binding capacity value of over 20 mg/mL), and have over 80% flow through pores in their pore structure contribute to low flow resistance. The resulted monolithic columns have promising potential for dual mode liquid chromatography. MATE may contribute for anion-exchange while VBC may responsible for reversed-phase liquid chromatography.

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Preparation of methacrylate-based anion-exchange monolithic microbore column for chromatographic separation of DNA fragments and oligonucleotides

Cited by 41 publications

References 50 publications

"DEVELOPMENT OF Ti4+-IMMOBILIZED NANOPOROUS MONOLITHIC POLYMER FOR SELECTIVE SEPARATION AND DETECTION OF PHOSPHOPEPTIDES "

"DEVELOPMENT OF Ti4+-IMMOBILIZED NANOPOROUS MONOLITHIC POLYMER FOR SELECTIVE SEPARATION AND DETECTION OF PHOSPHOPEPTIDES "

Preparasi Kolom Microbore Monolith Berbasis Polistirena untuk Pemisahan Alkilbenzena Menggunakan Kromatografi Cair Kinerja Tinggi

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

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