Preparation of Marine Plasmalogen and Selective Identification of Molecular Species by LC-MS/MS

Yamashita, Shinji; Honjo, Ayako; Aruga, Mizue; Nakagawa, Kiyotaka; Miyazawa, Teruo

doi:10.5650/jos.ess13188

Cited by 32 publications

(27 citation statements)

References 27 publications

(20 reference statements)

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“…Based on the substitution present at the sn-1 position of the glycerol structure [ 32 ], GPL are divided into three subclasses: acyl, alkyl and alkenyl [ 33 ]. The alkenyls formed are called plasmalogens or plasmenyls or 1-0(1Z-alkenyl)-2-acyl- glycerophospholipids [ 7 ].…”

Section: Characteristics Of Plasmalogensmentioning

confidence: 99%

“…Among the chromatographic methods used for plasmalogen analysis are Thin-layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) [ 33 ]. These techniques, based on relative or absolute quantification and the use of internal standards, help in the identification of different plasmalogen subspecies as well as new plasmalogens [ 7 ].…”

Section: Characteristics Of Plasmalogensmentioning

confidence: 99%

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Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

et al. 2018

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The plasmalogens are a class of glycerophospholipids which contain a vinyl-ether and an ester bond at the sn-1 and sn-2 positions, respectively, in the glycerol backbone. They constitute 10 mol% of the total mass of phospholipids in humans, mainly as membrane structure components. Plasmalogens are important for the organization and stability of lipid raft microdomains and cholesterol-rich membrane regions involved in cellular signaling. In addition to their structural roles, a subset of ether lipids are thought to function as endogenous antioxidants and emerging studies suggest that they are involved in cell differentiation and signaling pathways. Although the clinical significance of plasmalogens is linked to peroxisomal disorders, the pathophysiological roles and their possible metabolic pathways are not fully understood since they present unique structural attributes for the different tissue types. Studies suggest that changes in plasmalogen metabolism may contribute to the development of various types of cancer. Here, we review the molecular characteristics of plasmalogens in order to significantly increase our understanding of the plasmalogen molecule and its involvement in gastrointestinal cancers as well as other types of cancers.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0685-9) contains supplementary material, which is available to authorized users.

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Section: Characteristics Of Plasmalogensmentioning

confidence: 99%

Section: Characteristics Of Plasmalogensmentioning

confidence: 99%

Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

et al. 2018

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“…Recently, new methods with mass spectrometry (MS) were reported for determination of plasmalogen, such as GC-MS [41] and LC-MS/MS [42,43], and were shown to be applicable to measurement of plasmalogen with high sensitivity. Although these methods enable analysis of molecular species and fatty acids, it is difficult to obtain total plasmalogen because of the requirement for standards Although plasmalogen levels of EtnGpl in some marine invertebrates were higher in the present study than those reported previously [10,12,44], the differences were thought to be due to the instability of derivatives and sensitivity as described above.…”

Section: Discussionmentioning

confidence: 99%

Separation and Detection of Plasmalogen in Marine Invertebrates by High‐Performance Liquid Chromatography with Evaporative Light‐Scattering Detection

Yamashita

Abe

Nakagawa

et al. 2014

Lipids

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We have developed a new method for determining ethanolamine plasmalogen contents in marine invertebrates. This quantification method involves derivatization of ethanolamine glycerophospholipid (EtnGpl) subclasses, alkenylacyl (plasmalogen), diacyl, and alkylacyl subclasses, by enzyme treatment and acetylation, followed by separation and detection by high-performance liquid chromatography (HPLC) with evaporative light-scattering detection (ELSD). This method enabled complete separation of the subclasses, and the limit of detection for plasmalogen was 200 ng (260 pmol). The peak area of plasmalogen by ELSD was unaffected by the degree of unsaturated fatty acids in EtnGpl, in contrast to ultraviolet (UV) detection. Thus, this method enables accurate determination of plasmalogen contents in various species containing marine products possessing abundant polyunsaturated fatty acids (PUFA). The method developed here was applied to marine invertebrates available in Japan. The examined marine invertebrates showed a wide range of plasmalogen contents ranging from 19 to 504 μmol/100 g wet wt. The plasmalogen levels in samples except those of class Cephalopoda and Crustacea were more than 60 mol% of EtnGpl.

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“…These chemical properties have been recently leveraged for the characterization of marine plasmalogens . Thus, crushed freeze‐dried viscera samples from ascidian Halocynthia roretzi (i.e.…”

Section: Selective Isolation Of Plsmentioning

confidence: 99%

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

et al. 2018

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Lipids are gaining relevance over the last 20 years, as our knowledge about their role has changed from merely energy/structural molecules to compounds also involved in several biological processes. This led to the creation in 2003 of a new emerging research field: lipidomics. In particular the phospholipids have pharmacological/food applications, participate in cell signalling/homeostatic pathways while their analysis faces some challenges. Their fractionation/purification is, in fact, especially difficult, as they are amphiphilic compounds. Moreover, it usually involves SPE or TLC procedures requiring specific materials hampering their suitableness for routine analysis. Finally, they can interfere with the ionization of other molecules during mass spectrometry analysis. Thus, simple high-throughput reliable methods to selectively isolate these compounds based on the difference between chemical characteristics of lipids would represent valuable tools for their study besides that of other compounds. The current review work aims to describe the state-of-the-art related to the extraction of phospholipids using liquid-liquid methods for their targeted isolation. The technological and biological importance of these compounds and ion suppression phenomena are also reviewed. Methods by precipitation with acetone or isolation using methanol seem to be suitable for selective isolation of phospholipids in both biological and food samples.

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Preparation of Marine Plasmalogen and Selective Identification of Molecular Species by LC-MS/MS

Cited by 32 publications

References 27 publications

Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

Separation and Detection of Plasmalogen in Marine Invertebrates by High‐Performance Liquid Chromatography with Evaporative Light‐Scattering Detection

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

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