Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

Messias, Márcia Cristina Fernandes; Mecatti, Giovana Colozza; Priolli, Denise Gonçalves; Carvalho, Patrícia de Oliveira

doi:10.1186/s12944-018-0685-9

Cited by 141 publications

(150 citation statements)

References 103 publications

(121 reference statements)

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“…Plasmalogens play many crucial roles in cellular functions, including reservoirs for second messengers, working as endogenous antioxidants, as well as the structural components of cellular membranes [34,35]. The aberrant metabolism of plasmalogens had been found in Alzheimer's disease [36], Parkinson's disease [37], Niemann-Pick disease [38], and cancers [39]. The role of plasmalogens in mitochondrial disorders remains largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Improved Dried Blood Spot-Based Metabolomics: A Targeted, Broad-Spectrum, Single-Injection Method

Naviaux

Monk

et al. 2020

Metabolites

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Dried blood spots (DBS) have proven to be a powerful sampling and storage method for newborn screening and many other applications. However, DBS methods have not yet been optimized for broad-spectrum targeted metabolomic analysis. In this study, we developed a robust, DBS-based, broad-spectrum, targeted metabolomic method that was able to measure over 400 metabolites from a 6.3 mm punch from standard Whatman 903TM filter paper cards. The effects of blood spot volumes, hematocrit, vacutainer chemistry, extraction methods, carryover, and comparability with plasma and fingerstick capillary blood samples were analyzed. The stability of over 400 metabolites stored under varying conditions over one year was also tested. No significant impacts of blood volume and hematocrit variations were observed when the spotted blood volume was over 60 µL and the hematocrit was between 31% and 50%. The median area under the curve (AUC) of metabolites in the DBS metabolome declined by 40% in the first 3 months and then did not decline further for at least 1 year. All originally detectable metabolites remained within detectable limits. The optimal storage conditions for metabolomic analysis were −80 °C with desiccants and without an O2 scavenger. The method was clinically validated for its potential utility in the diagnosis of the mitochondrial disease mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS). Our method provides a convenient alternative to freezing, storing, and shipping liquid blood samples for comparative metabolomic studies.

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Section: Discussionmentioning

confidence: 99%

Improved Dried Blood Spot-Based Metabolomics: A Targeted, Broad-Spectrum, Single-Injection Method

Naviaux

Monk

et al. 2020

Metabolites

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“…Although ether lipids have been estimated to represent 18% of total phospholipids in humans [33], they have so far not obtained much attention, and they are still hardly mentioned in text books. Ether lipids have been reported to be involved in membrane trafficking, cell signaling, to possibly function as cellular antioxidants and to be enriched in cancer cells; we refer to the following review articles regarding ether lipids [33][34][35][36]. Importantly, the ether lipids are required for generation of the alkyl-containing glycosylphosphatidylinositol (GPI)-anchored proteins, which can be present in lipid rafts [26,37].…”

Section: Ether Lipidsmentioning

confidence: 99%

The role of lipid species in membranes and cancer-related changes

Skotland

Kavaliauskiene

Sandvig

2020

Cancer Metastasis Rev

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Several studies have demonstrated interactions between the two leaflets in membrane bilayers and the importance of specific lipid species for such interaction and membrane function. We here discuss these investigations with a focus on the sphingolipid and cholesterol-rich lipid membrane domains called lipid rafts, including the small flask-shaped invaginations called caveolae, and the importance of such membrane structures in cell biology and cancer. We discuss the possible interactions between the very long-chain sphingolipids in the outer leaflet of the plasma membrane and the phosphatidylserine species PS 18:0/18:1 in the inner leaflet and the importance of cholesterol for such interactions. We challenge the view that lipid rafts contain a large fraction of lipids with two saturated fatty acyl groups and argue that it is important in future studies of membrane models to use asymmetric membrane bilayers with lipid species commonly found in cellular membranes. We also discuss the need for more quantitative lipidomic studies in order to understand membrane function and structure in general, and the importance of lipid rafts in biological systems. Finally, we discuss cancer-related changes in lipid rafts and lipid composition, with a special focus on changes in glycosphingolipids and the possibility of using lipid therapy for cancer treatment.

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“…Interestingly, it was proposed that some specific lipids in the plasma could be used as specific biomarkers 12 of prostate cancer, such as alkyl-glycerophosphocholine 10 . Similar studies have been obtained for breast cancer (132).…”

Section: Ether Lipidsmentioning

confidence: 99%

“…At that time, these lipids had been found to regulate cell proliferation (10). This was only a few decades ago and thanks to advanced research technologies more accurate EL compositions (identification of molecular species of ELs) have been described in tumor tissues (11,12). These observations went with the discovery of new functions of ELs in cancer biology, such as carcinogenesis, progression, migration and invasion (13,14).…”

Section: Introductionmentioning

confidence: 99%

Roles of endogenous ether lipids and associated PUFAs in the regulation of ion channels and their relevance for disease

Fontaine

Figiel

Félix

et al. 2020

Journal of Lipid Research

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Ether lipids (ELs) are lipids characterized by the presence of either an ether linkage (alkyl lipids) or a vinyl ether linkage [i.e., plasmalogens (Pls)] at the sn1 position of the glycerol backbone, and they are enriched in PUFAs at the sn2 position. In this review, we highlight that ELs have various biological functions, act as a reservoir for second messengers (such as PUFAs) and have roles in many diseases. Some of the biological effects of ELs may be associated with their ability to regulate ion channels that control excitation-contraction/secretion/mobility coupling and therefore cell physiology. These channels are embedded in lipid membranes, and lipids can regulate their activities directly or indirectly as second messengers or by incorporating into membranes. Interestingly, ELs and EL-derived PUFAs have been reported to play a key role in several pathologies, including neurological disorders, cardiovascular diseases, and cancers. Investigations leading to a better understanding of their mechanisms of action in pathologies have opened a new field in cancer research. In summary, newly identified lipid regulators of ion channels, such as ELs and PUFAs, may represent valuable targets to improve disease diagnosis and advance the development of new therapeutic strategies for managing a range of diseases and conditions.

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Plasmalogen lipids: functional mechanism and their involvement in gastrointestinal cancer

Cited by 141 publications

References 103 publications

Improved Dried Blood Spot-Based Metabolomics: A Targeted, Broad-Spectrum, Single-Injection Method

Improved Dried Blood Spot-Based Metabolomics: A Targeted, Broad-Spectrum, Single-Injection Method

The role of lipid species in membranes and cancer-related changes

Roles of endogenous ether lipids and associated PUFAs in the regulation of ion channels and their relevance for disease

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