Preparation of LL-37-Grafted Titanium Surfaces with Bactericidal Activity

Gabriel, Matthias; Nazmi, Kamran; Veerman, E.C.I.; Amerongen, and Arie V. Nieuw; Zentner, Andrej

doi:10.1021/bc050091v

Cited by 187 publications

(231 citation statements)

References 15 publications

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“…Humblot et al [68] reported that the immobilized AMPs had a bacteriostatic rather that bactericidal effect, perhaps due to the low peptide concentration or short contact time. Other research has performed the immobilization through long linkers in an attempt to permit sufficient flexibility to penetrate target cell membranes [1,69,70], as the directly attached AMPs lost their antimicrobial activity. Hilpert et al [71], in their screening and characterization of surface-tethered cationic peptides studies, reported that the immobilization of peptides to a surface should result in constraints on the peptide mobility and on the capacity of peptides to enter or even transpose the cellular membranes.…”

Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

“…Other important activity-modulating parameters include the length, flexibility and kind of spacer between the active sequences and the solid matrices [67,69,70], the AMP surface density [1,68,69], and orientation after immobilization [1,11,[67][68][69][70][76][77][78]. These parameters are discussed below.…”

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…silanized titanium) [70], glass coverslips [76], model surfaces (e.g. self-assembled monolayers) [68], microtitre plates [71] and even commercial contact lenses [11].…”

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

“…Although some studies demonstrated that immobilized AMPs have antimicrobial activity without incorporation of a spacer [11,68,71,76], most protocols presented a spacer attachment step [1,56,67,69,70,77,78], particularly with a PEG spacer with a MW ranging from 3000 to 5400 [56,69,70,77,78]. The utilization of PEG as a spacer presents several advantages.…”

Section: Influence Of the Spacermentioning

confidence: 99%

“…This polymer can create non-adhesive surfaces due to its non-fouling characteristics, thus preventing non-specific peptide binding to the surface and shielding the peptides from the hydrophobic nature of a particular biomaterial [56,77]. Studies comparing AMP immobilization with and without PEGylated spacers demonstrated that some immobilized AMPs are only bactericidal when a PEGylated spacer was used [56,69,70,77]. For example, Gabriel et al [70] demonstrated that the LL37 peptide bound to titanium through a PEGylated spacer was capable of killing Escherichia coli on contact.…”

Section: Influence Of the Spacermentioning

confidence: 99%

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Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

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Section: Mode Of Antibacterial Actionmentioning

confidence: 99%

Section: Covalent Immobilization Of An Antimicrobial Peptidementioning

confidence: 99%

“…silanized titanium) [70], glass coverslips [76], model surfaces (e.g. self-assembled monolayers) [68], microtitre plates [71] and even commercial contact lenses [11].…”

Section: Solid Supports and Chemical Coupling Strategiesmentioning

confidence: 99%

Section: Influence Of the Spacermentioning

confidence: 99%

Section: Influence Of the Spacermentioning

confidence: 99%

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Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

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Cationic Antimicrobial Peptides

Phoenix

Dennison

Harris

2013

Antimicrobial Peptides

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Antimicrobial Potential of Copper‐Containing Titanium Surfaces Generated by Ion Implantation and Dual High Power Impulse Magnetron Sputtering

et al. 2011

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The application of antimicrobial surfaces to titanium alloy (Ti) implants would be beneficial to prevent implant‐associated infections of joint endoprostheses and osteosyntheses. Copper (Cu) could be advantageously applied for this purpose, since it exhibits a well‐known antimicrobial activity and is a trace element in the human body, i.e., it is non‐toxic in small concentrations. This approach was evaluated with two plasma‐based surface modification procedures: Implantation of Cu ions into Ti by means of plasma immersion ion implantation (PIII) and Coating of Ti surfaces with CuTi films by means of dual high power impulse magnetron sputtering (dual HiPIMS). In this manner, the surfaces could be equipped with various amounts of Cu, as it was analyzed by X‐ray photoelectron spectroscopy (XPS). The surfaces released up to 8 mmol · L−1 of Cu within 24 h, measured with atomic absorption spectroscopy (AAS). Hence, the surfaces possessed an antimicrobial potential against typical infect‐associated bacteria (Staphylococcus aureus). Surfaces with a higher Cu release prepared by HiPIMS technique revealed a higher antimicrobial effect, while surfaces implanted by PIII were less cytotoxic to osteoblasts (MG‐63 cells). These results show that Cu doped and coated implants could be useful for prevention and therapy of implant‐associated infections.

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Preparation of LL-37-Grafted Titanium Surfaces with Bactericidal Activity

Cited by 187 publications

References 15 publications

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Cationic Antimicrobial Peptides

Antimicrobial Potential of Copper‐Containing Titanium Surfaces Generated by Ion Implantation and Dual High Power Impulse Magnetron Sputtering

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