Preparation of liposomes: A novel application of microengineered membranes–From laboratory scale to large scale

Laouini, A.; Charcosset, Catherine; Fessi, Hatem; Holdich, Richard; Vladisavljević, Goran T.

doi:10.1016/j.colsurfb.2013.07.066

Cited by 55 publications

(27 citation statements)

References 27 publications

(29 reference statements)

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“…Therefore, a larger number of nuclei will lead to smaller size of NPs. The smaller particle sizes at higher aqueous-to-organic volume ratios were also obtained by Laouini et al (2013aLaouini et al ( , 2013bLaouini et al ( , 2013c in the production of liposomes and polymeric micelles in membrane contactors and by Jahn et al (2010) in the formation of liposomes in planar flow focusing microfluidic mixers.…”

Section: Effects Of Aqueous-to-organic Flowmentioning

confidence: 94%

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Othman

Vladisavljević

Nagy

2015

Chemical Engineering Science

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The aim of this study was to develop a new microfluidic approach for the preparation of nanoparticles with tuneable sizes based on micromixing / direct nanoprecipitation in a coaxial assembly of tapered-end glass capillaries. The organic phase was 1 wt% poly(-caprolactone) (PCL) or poly(dl-lactic acid) (PLA) in tetrahydrofuran and the antisolvent was Milli-Q water.The size of nanoparticles was precisely controlled over a range of 190-650 nm by controlling phase flow rates, orifice size and flow configuration (two-phase co-flow or countercurrent flow focusing). Smaller particles were produced in a flow focusing device, because the organic phase stream was significantly narrower than the orifice and remained narrow for a longer distance downstream of the orifice. The mean size of PCL particles produced in a flow focusing device with an orifice size of 200 m, an organic phase flow rate of 1.7 mL h -1 and an aqueous-toorganic flow rate ratio of 10 was below 200 nm. The size of nanoparticles decreased with decreasing the orifice size and increasing the aqueous-to-organic phase flow rate ratio. Due to *Revised Manuscript Click here to view linked References 2 higher affinity for water and amorphous structure, PLA nanoparticles were smaller and exhibited a smoother surface and more rounded shape than PCL particles.

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Section: Effects Of Aqueous-to-organic Flowmentioning

confidence: 94%

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Othman

Vladisavljević

Nagy

2015

Chemical Engineering Science

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“…In the oscillating membrane system, the ring membrane, oscillating with 40 Hz frequency and 1.2 mm amplitude, was immersed into a beaker containing 480 ml of the aqueous phase and 107 ml of the organic phase was injected through the membrane at 8 ml min and an aqueous to organic phase volume ratio before ethanol evaporation of 4.5. The above operating parameters were chosen based on the previously done process optimisation step [20,23].…”

Section: Membrane Dispersion Devicesmentioning

confidence: 99%

Production of liposomes using microengineered membrane and co-flow microfluidic device

Vladisavljević

Laouini

Charcosset

et al. 2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…The same trend was observed for itraconazole and ZnO NPs prepared via SPG membrane, 42,43 and liposomes and micelles produced using nickel membrane. 27,44,45 . The size of NPs formed by anti-solvent precipitation is a result of the relative rates of nucleation, particle growth, and agglomeration.…”

Section: Resultsmentioning

confidence: 99%

Encapsulation and Controlled Release of Rapamycin from Polycaprolactone Nanoparticles Prepared by Membrane Micromixing Combined with Antisolvent Precipitation

et al. 2016

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Rapamycin-loaded polycaprolactone nanoparticles (RAPA-PCL NPs) with a polydispersity index of 0.006–0.073 were fabricated by antisolvent precipitation combined with micromixing using a ringed stainless steel membrane with 10 μm diameter laser-drilled pores. The organic phase composed of 6 g L–1 PCL and 0.6–3.0 g L–1 RAPA in acetone was injected through the membrane at 140 L m–2 h–1 into 0.2 wt % aqueous poly(vinyl alcohol) solution stirred at 1300 rpm, resulting in a Z-average mean of 189–218 nm, a drug encapsulation efficiency of 98.8–98.9%, and a drug loading in the NPs of 9–33%. The encapsulation of RAPA was confirmed by UV–vis spectroscopy, XRD, DSC, and ATR-FTIR. The disappearance of sharp characteristic peaks of crystalline RAPA in the XRD pattern of RAPA-PCL NPs revealed that the drug was molecularly dispersed in the polymer matrix or RAPA and PCL were present in individual amorphous domains. The rate of drug release in pure water was negligible due to low aqueous solubility of RAPA. RAPA-PCL NPs released more than 91% of their drug cargo after 2.5 h in the release medium composed of 0.78–1.5 M of the hydrotropic agent N,N-diethylnicotinamide, 10 vol % ethanol, and 2 vol % Tween 20 in phosphate buffered saline. The dissolution of RAPA was slower when the drug was embedded in the PCL matrix of the NPs than dispersed in the form of pure RAPA nanocrystals.

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Preparation of liposomes: A novel application of microengineered membranes–From laboratory scale to large scale

Cited by 55 publications

References 27 publications

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Production of liposomes using microengineered membrane and co-flow microfluidic device

Encapsulation and Controlled Release of Rapamycin from Polycaprolactone Nanoparticles Prepared by Membrane Micromixing Combined with Antisolvent Precipitation

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