Production of liposomes using microengineered membrane and co-flow microfluidic device

Vladisavljević, Goran T.; Laouini, A.; Charcosset, Catherine; Fessi, Hatem; Bandulasena, Hemaka C.H.; Holdich, Richard

doi:10.1016/j.colsurfa.2014.03.016

Cited by 41 publications

(32 citation statements)

References 28 publications

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“…In this device, the mixing occurs at the microscale level, because the membrane provides numerous microinjection points to finely disperse the injected phase into the stirred phase without intermediate macromixing stage [33]. To prepare the chitosan-TPP nanoparticles, the TPP solution was injected through the membrane pores into the chitosan solution contained in the stirred cell.…”

Section: Optimization Of Process Parametersmentioning

confidence: 99%

Preparation of chitosan–TPP nanoparticles using microengineered membranes – Effect of parameters and encapsulation of tacrine

Hassani

Laouini

Fessi

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

110

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Section: Optimization Of Process Parametersmentioning

confidence: 99%

Preparation of chitosan–TPP nanoparticles using microengineered membranes – Effect of parameters and encapsulation of tacrine

Hassani

Laouini

Fessi

et al. 2015

Colloids and Surfaces A: Physicochemical and Engineering Aspect

110

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“…The penetration of the less viscous fluid is not uniform since part of the more viscous fluid forms fjords, named "viscous fingers" (Homsy, 1987). Viscous fingering was not observed when ethanolic solution of phospholipids was injected into water in the same type of capillary device (Vladisavljević et al, 2014), because the viscosity of ethanol of 1.25 mPa s was higher than the water viscosity. At or aq Q Q / = 1.5, two symmetrical vortices were formed at the lower and upper parts of the capillary tube (Fig.…”

Section: Effects Of Aqueous-to-organic Flowmentioning

confidence: 99%

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Othman

Vladisavljević

Nagy

2015

Chemical Engineering Science

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The aim of this study was to develop a new microfluidic approach for the preparation of nanoparticles with tuneable sizes based on micromixing / direct nanoprecipitation in a coaxial assembly of tapered-end glass capillaries. The organic phase was 1 wt% poly(-caprolactone) (PCL) or poly(dl-lactic acid) (PLA) in tetrahydrofuran and the antisolvent was Milli-Q water.The size of nanoparticles was precisely controlled over a range of 190-650 nm by controlling phase flow rates, orifice size and flow configuration (two-phase co-flow or countercurrent flow focusing). Smaller particles were produced in a flow focusing device, because the organic phase stream was significantly narrower than the orifice and remained narrow for a longer distance downstream of the orifice. The mean size of PCL particles produced in a flow focusing device with an orifice size of 200 m, an organic phase flow rate of 1.7 mL h -1 and an aqueous-toorganic flow rate ratio of 10 was below 200 nm. The size of nanoparticles decreased with decreasing the orifice size and increasing the aqueous-to-organic phase flow rate ratio. Due to *Revised Manuscript Click here to view linked References 2 higher affinity for water and amorphous structure, PLA nanoparticles were smaller and exhibited a smoother surface and more rounded shape than PCL particles.

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“…The solvent-PCL distance in the "2δ d −δ p −δ h " solubility space is equal to 1.08 and 6.53 for THF and acetone, respectively, 20 which means that PCL is more soluble in THF than acetone. Thus, compared to acetone, THF must be diluted with water to a greater extent for PCL to begin to precipitate, which means that the growing NPs are less likely to agglomerate.…”

Section: Resultsmentioning

confidence: 99%

“…17 Size-tuneable NPs can be prepared by nanoprecipitation/micromixing using microporous membrane 18,19 or microfluidic channels. 20,21 In membrane micromixing, the organic phase is introduced into the aqueous phase through a microporous membrane under controlled injection rate and shear on the membrane/aqueous phase interface. Here, the role of membrane is to uniformly split the organic phase stream into numerous microstreams, allowing for more uniform mixing conditions compared to direct injection through a single injection point, e.g.…”

Section: Encapsulation and Controlled Release Of Rapamycin From Polycmentioning

confidence: 99%

Encapsulation and Controlled Release of Rapamycin from Polycaprolactone Nanoparticles Prepared by Membrane Micromixing Combined with Antisolvent Precipitation

et al. 2016

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Rapamycin-loaded polycaprolactone nanoparticles (RAPA-PCL NPs) with a polydispersity index of 0.006–0.073 were fabricated by antisolvent precipitation combined with micromixing using a ringed stainless steel membrane with 10 μm diameter laser-drilled pores. The organic phase composed of 6 g L–1 PCL and 0.6–3.0 g L–1 RAPA in acetone was injected through the membrane at 140 L m–2 h–1 into 0.2 wt % aqueous poly(vinyl alcohol) solution stirred at 1300 rpm, resulting in a Z-average mean of 189–218 nm, a drug encapsulation efficiency of 98.8–98.9%, and a drug loading in the NPs of 9–33%. The encapsulation of RAPA was confirmed by UV–vis spectroscopy, XRD, DSC, and ATR-FTIR. The disappearance of sharp characteristic peaks of crystalline RAPA in the XRD pattern of RAPA-PCL NPs revealed that the drug was molecularly dispersed in the polymer matrix or RAPA and PCL were present in individual amorphous domains. The rate of drug release in pure water was negligible due to low aqueous solubility of RAPA. RAPA-PCL NPs released more than 91% of their drug cargo after 2.5 h in the release medium composed of 0.78–1.5 M of the hydrotropic agent N,N-diethylnicotinamide, 10 vol % ethanol, and 2 vol % Tween 20 in phosphate buffered saline. The dissolution of RAPA was slower when the drug was embedded in the PCL matrix of the NPs than dispersed in the form of pure RAPA nanocrystals.

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Production of liposomes using microengineered membrane and co-flow microfluidic device

Cited by 41 publications

References 28 publications

Preparation of chitosan–TPP nanoparticles using microengineered membranes – Effect of parameters and encapsulation of tacrine

Preparation of chitosan–TPP nanoparticles using microengineered membranes – Effect of parameters and encapsulation of tacrine

Preparation of biodegradable polymeric nanoparticles for pharmaceutical applications using glass capillary microfluidics

Encapsulation and Controlled Release of Rapamycin from Polycaprolactone Nanoparticles Prepared by Membrane Micromixing Combined with Antisolvent Precipitation

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